Cargando…
Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk
In this study, we investigated the species composition and diversity of psychrotrophic bacteria in raw milk from Heilongjiang, Inner Mongolia, Gansu, Henan, Anhui, Jiangsu, Chongqing, and Hunan provinces in China using traditional cultivation and PacBio Single Molecule Real-Time sequencing methods....
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866939/ https://www.ncbi.nlm.nih.gov/pubmed/35222348 http://dx.doi.org/10.3389/fmicb.2022.853263 |
_version_ | 1784655943663878144 |
---|---|
author | Du, Bingyao Meng, Lu Liu, Huimin Zheng, Nan Zhang, Yangdong Zhao, Shengguo Wang, Jiaqi |
author_facet | Du, Bingyao Meng, Lu Liu, Huimin Zheng, Nan Zhang, Yangdong Zhao, Shengguo Wang, Jiaqi |
author_sort | Du, Bingyao |
collection | PubMed |
description | In this study, we investigated the species composition and diversity of psychrotrophic bacteria in raw milk from Heilongjiang, Inner Mongolia, Gansu, Henan, Anhui, Jiangsu, Chongqing, and Hunan provinces in China using traditional cultivation and PacBio Single Molecule Real-Time sequencing methods. The isolated psychrotrophic bacteria were highly diverse, which composed of 21 genera and 59 species. Pseudomonas accounted for 58.9% of the total genera while Stenotrophomonas and Enterococcus were also highly represented (above 5.0%). In particular, P. azotoformans occurred at a level of 16.9% and P. paralactis, P. lactis, E. faecalis, and P. marginalis were present in relatively high proportions (above 4.0%). Regional differences were found significantly among the test regions except samples from Heilongjiang and Inner Mongolia were similar. Additionally, differences were observed between days in Henan, Anhui, and Jiangsu samples. Therefore, control strategies must be implemented on regional and season basis. |
format | Online Article Text |
id | pubmed-8866939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88669392022-02-25 Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk Du, Bingyao Meng, Lu Liu, Huimin Zheng, Nan Zhang, Yangdong Zhao, Shengguo Wang, Jiaqi Front Microbiol Microbiology In this study, we investigated the species composition and diversity of psychrotrophic bacteria in raw milk from Heilongjiang, Inner Mongolia, Gansu, Henan, Anhui, Jiangsu, Chongqing, and Hunan provinces in China using traditional cultivation and PacBio Single Molecule Real-Time sequencing methods. The isolated psychrotrophic bacteria were highly diverse, which composed of 21 genera and 59 species. Pseudomonas accounted for 58.9% of the total genera while Stenotrophomonas and Enterococcus were also highly represented (above 5.0%). In particular, P. azotoformans occurred at a level of 16.9% and P. paralactis, P. lactis, E. faecalis, and P. marginalis were present in relatively high proportions (above 4.0%). Regional differences were found significantly among the test regions except samples from Heilongjiang and Inner Mongolia were similar. Additionally, differences were observed between days in Henan, Anhui, and Jiangsu samples. Therefore, control strategies must be implemented on regional and season basis. Frontiers Media S.A. 2022-02-10 /pmc/articles/PMC8866939/ /pubmed/35222348 http://dx.doi.org/10.3389/fmicb.2022.853263 Text en Copyright © 2022 Du, Meng, Liu, Zheng, Zhang, Zhao and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Du, Bingyao Meng, Lu Liu, Huimin Zheng, Nan Zhang, Yangdong Zhao, Shengguo Wang, Jiaqi Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk |
title | Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk |
title_full | Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk |
title_fullStr | Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk |
title_full_unstemmed | Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk |
title_short | Single Molecule Real-Time Sequencing and Traditional Cultivation Techniques Reveal Complex Community Structures and Regional Variations of Psychrotrophic Bacteria in Raw Milk |
title_sort | single molecule real-time sequencing and traditional cultivation techniques reveal complex community structures and regional variations of psychrotrophic bacteria in raw milk |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866939/ https://www.ncbi.nlm.nih.gov/pubmed/35222348 http://dx.doi.org/10.3389/fmicb.2022.853263 |
work_keys_str_mv | AT dubingyao singlemoleculerealtimesequencingandtraditionalcultivationtechniquesrevealcomplexcommunitystructuresandregionalvariationsofpsychrotrophicbacteriainrawmilk AT menglu singlemoleculerealtimesequencingandtraditionalcultivationtechniquesrevealcomplexcommunitystructuresandregionalvariationsofpsychrotrophicbacteriainrawmilk AT liuhuimin singlemoleculerealtimesequencingandtraditionalcultivationtechniquesrevealcomplexcommunitystructuresandregionalvariationsofpsychrotrophicbacteriainrawmilk AT zhengnan singlemoleculerealtimesequencingandtraditionalcultivationtechniquesrevealcomplexcommunitystructuresandregionalvariationsofpsychrotrophicbacteriainrawmilk AT zhangyangdong singlemoleculerealtimesequencingandtraditionalcultivationtechniquesrevealcomplexcommunitystructuresandregionalvariationsofpsychrotrophicbacteriainrawmilk AT zhaoshengguo singlemoleculerealtimesequencingandtraditionalcultivationtechniquesrevealcomplexcommunitystructuresandregionalvariationsofpsychrotrophicbacteriainrawmilk AT wangjiaqi singlemoleculerealtimesequencingandtraditionalcultivationtechniquesrevealcomplexcommunitystructuresandregionalvariationsofpsychrotrophicbacteriainrawmilk |