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Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation

Neisseria gonorrhoeae infection of the female lower genital tract can present with a spectrum of phenotypes ranging from asymptomatic carriage to symptomatic cervical inflammation, or cervicitis. The factors that contribute to the development of asymptomatic or symptomatic infections are largely unc...

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Autores principales: Lovett, Angela, Seña, Arlene C., Macintyre, Andrew N., Sempowski, Gregory D., Duncan, Joseph A., Waltmann, Andreea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867028/
https://www.ncbi.nlm.nih.gov/pubmed/35222300
http://dx.doi.org/10.3389/fmicb.2021.790531
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author Lovett, Angela
Seña, Arlene C.
Macintyre, Andrew N.
Sempowski, Gregory D.
Duncan, Joseph A.
Waltmann, Andreea
author_facet Lovett, Angela
Seña, Arlene C.
Macintyre, Andrew N.
Sempowski, Gregory D.
Duncan, Joseph A.
Waltmann, Andreea
author_sort Lovett, Angela
collection PubMed
description Neisseria gonorrhoeae infection of the female lower genital tract can present with a spectrum of phenotypes ranging from asymptomatic carriage to symptomatic cervical inflammation, or cervicitis. The factors that contribute to the development of asymptomatic or symptomatic infections are largely uncharacterized. We conducted a pilot study to assess differences in the cervicovaginal microbial community of patients presenting with symptomatic vs. asymptomatic N. gonorrhoeae infections to a sexually transmitted infections (STI) clinic. DNA was isolated from cervicovaginal swab specimens from women who tested positive for N. gonorrhoeae infection using a clinical diagnostic nucleic acid amplification test. We performed deep sequencing of 16S ribosomal RNA gene amplicons, followed by microbiome analyses with QIIME, and species-specific real-time PCR to assess the composition of microbial communities cohabitating the lower genital tract with the infecting N. gonorrhoeae. Specimens collected from asymptomatic individuals with N. gonorrhoeae infection and no co-infection with Chlamydia trachomatis and/or Trichomonas vaginalis carried Lactobacillus-dominant microbial communities more frequently than symptomatic patients without co-infection. When compared to asymptomatic individuals, symptomatic women had microbial communities characterized by more diverse and heterogenous bacterial taxa, typically associated with bacterial vaginosis (BV) [Prevotella, Sneathia, Mycoplasma hominis, and Bacterial Vaginosis-Associated Bacterium-1 (BVAB1)/“Candidatus Lachnocurva vaginae”]. Both symptomatic and asymptomatic N. gonorrhoeae patients with additional STI co-infection displayed a BV-like microbial community. These findings suggest that Lactobacillus-dominant vaginal microbial community may protect individuals from developing symptoms during lower genital tract infection with N. gonorrhoeae.
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spelling pubmed-88670282022-02-25 Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation Lovett, Angela Seña, Arlene C. Macintyre, Andrew N. Sempowski, Gregory D. Duncan, Joseph A. Waltmann, Andreea Front Microbiol Microbiology Neisseria gonorrhoeae infection of the female lower genital tract can present with a spectrum of phenotypes ranging from asymptomatic carriage to symptomatic cervical inflammation, or cervicitis. The factors that contribute to the development of asymptomatic or symptomatic infections are largely uncharacterized. We conducted a pilot study to assess differences in the cervicovaginal microbial community of patients presenting with symptomatic vs. asymptomatic N. gonorrhoeae infections to a sexually transmitted infections (STI) clinic. DNA was isolated from cervicovaginal swab specimens from women who tested positive for N. gonorrhoeae infection using a clinical diagnostic nucleic acid amplification test. We performed deep sequencing of 16S ribosomal RNA gene amplicons, followed by microbiome analyses with QIIME, and species-specific real-time PCR to assess the composition of microbial communities cohabitating the lower genital tract with the infecting N. gonorrhoeae. Specimens collected from asymptomatic individuals with N. gonorrhoeae infection and no co-infection with Chlamydia trachomatis and/or Trichomonas vaginalis carried Lactobacillus-dominant microbial communities more frequently than symptomatic patients without co-infection. When compared to asymptomatic individuals, symptomatic women had microbial communities characterized by more diverse and heterogenous bacterial taxa, typically associated with bacterial vaginosis (BV) [Prevotella, Sneathia, Mycoplasma hominis, and Bacterial Vaginosis-Associated Bacterium-1 (BVAB1)/“Candidatus Lachnocurva vaginae”]. Both symptomatic and asymptomatic N. gonorrhoeae patients with additional STI co-infection displayed a BV-like microbial community. These findings suggest that Lactobacillus-dominant vaginal microbial community may protect individuals from developing symptoms during lower genital tract infection with N. gonorrhoeae. Frontiers Media S.A. 2022-02-10 /pmc/articles/PMC8867028/ /pubmed/35222300 http://dx.doi.org/10.3389/fmicb.2021.790531 Text en Copyright © 2022 Lovett, Seña, Macintyre, Sempowski, Duncan and Waltmann. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lovett, Angela
Seña, Arlene C.
Macintyre, Andrew N.
Sempowski, Gregory D.
Duncan, Joseph A.
Waltmann, Andreea
Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation
title Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation
title_full Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation
title_fullStr Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation
title_full_unstemmed Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation
title_short Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation
title_sort cervicovaginal microbiota predicts neisseria gonorrhoeae clinical presentation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867028/
https://www.ncbi.nlm.nih.gov/pubmed/35222300
http://dx.doi.org/10.3389/fmicb.2021.790531
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