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Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin

The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. While some effector proteins specifi...

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Autores principales: Amaya, Fernando A., Blondel, Carlos J., Barros-Infante, María F., Rivera, Dácil, Moreno-Switt, Andrea I., Santiviago, Carlos A., Pezoa, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867033/
https://www.ncbi.nlm.nih.gov/pubmed/35222335
http://dx.doi.org/10.3389/fmicb.2022.811932
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author Amaya, Fernando A.
Blondel, Carlos J.
Barros-Infante, María F.
Rivera, Dácil
Moreno-Switt, Andrea I.
Santiviago, Carlos A.
Pezoa, David
author_facet Amaya, Fernando A.
Blondel, Carlos J.
Barros-Infante, María F.
Rivera, Dácil
Moreno-Switt, Andrea I.
Santiviago, Carlos A.
Pezoa, David
author_sort Amaya, Fernando A.
collection PubMed
description The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). In Salmonella, five T6SS gene clusters have been identified within pathogenicity islands SPI-6, SPI-19, SPI-20, SPI-21, and SPI-22, which are differentially distributed among serotypes. Salmonella enterica serotype Dublin (S. Dublin) is a cattle-adapted pathogen that harbors both T6SS(SPI-6) and T6SS(SPI-19). Interestingly, while both systems have been linked to virulence and host colonization in S. Dublin, an antibacterial activity has not been detected for T6SS(SPI-6) in this serotype. In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS(SPI-6) and T6SS(SPI-19) gene clusters in S. Dublin. In the present study, we demonstrate that T6SS(SPI-6) and T6SS(SPI-19) of S. Dublin CT_02021853 contribute to interbacterial competition. Bioinformatic and comparative genomic analyses allowed us to identify genes encoding three candidate antibacterial effectors located within SPI-6 and two candidate effectors located within SPI-19. Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. Finally, we demonstrate that E/I modules SED_RS01930/SED_RS01935 (encoded in SPI-6), SED_RS06235/SED_RS06230, and SED_RS06335/SED_RS06340 (both encoded in SPI-19) contribute to interbacterial competition in S. Dublin CT_02021853.
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spelling pubmed-88670332022-02-25 Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin Amaya, Fernando A. Blondel, Carlos J. Barros-Infante, María F. Rivera, Dácil Moreno-Switt, Andrea I. Santiviago, Carlos A. Pezoa, David Front Microbiol Microbiology The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). In Salmonella, five T6SS gene clusters have been identified within pathogenicity islands SPI-6, SPI-19, SPI-20, SPI-21, and SPI-22, which are differentially distributed among serotypes. Salmonella enterica serotype Dublin (S. Dublin) is a cattle-adapted pathogen that harbors both T6SS(SPI-6) and T6SS(SPI-19). Interestingly, while both systems have been linked to virulence and host colonization in S. Dublin, an antibacterial activity has not been detected for T6SS(SPI-6) in this serotype. In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS(SPI-6) and T6SS(SPI-19) gene clusters in S. Dublin. In the present study, we demonstrate that T6SS(SPI-6) and T6SS(SPI-19) of S. Dublin CT_02021853 contribute to interbacterial competition. Bioinformatic and comparative genomic analyses allowed us to identify genes encoding three candidate antibacterial effectors located within SPI-6 and two candidate effectors located within SPI-19. Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. Finally, we demonstrate that E/I modules SED_RS01930/SED_RS01935 (encoded in SPI-6), SED_RS06235/SED_RS06230, and SED_RS06335/SED_RS06340 (both encoded in SPI-19) contribute to interbacterial competition in S. Dublin CT_02021853. Frontiers Media S.A. 2022-02-10 /pmc/articles/PMC8867033/ /pubmed/35222335 http://dx.doi.org/10.3389/fmicb.2022.811932 Text en Copyright © 2022 Amaya, Blondel, Barros-Infante, Rivera, Moreno-Switt, Santiviago and Pezoa. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Amaya, Fernando A.
Blondel, Carlos J.
Barros-Infante, María F.
Rivera, Dácil
Moreno-Switt, Andrea I.
Santiviago, Carlos A.
Pezoa, David
Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin
title Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin
title_full Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin
title_fullStr Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin
title_full_unstemmed Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin
title_short Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin
title_sort identification of type vi secretion systems effector proteins that contribute to interbacterial competition in salmonella dublin
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867033/
https://www.ncbi.nlm.nih.gov/pubmed/35222335
http://dx.doi.org/10.3389/fmicb.2022.811932
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