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The sustained PGE(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model

BACKGROUND: The promising therapeutic strategy for the treatment of peripheral artery disease (PAD) is to restore blood supply and promote regeneration of skeletal muscle regeneration. Increasing evidence revealed that prostaglandin E(2) (PGE(2)), a lipid signaling molecule, has significant therapeu...

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Autores principales: Huang, Haoyan, Chen, Shang, Cheng, Hui, Cao, Jiasong, Du, Wei, Zhang, Jun, Chang, Yuqiao, Shen, Xiaohong, Guo, Zhikun, Han, Zhibo, Hua, Guoqiang, Han, Zhong-Chao, Benkirane-Jessel, Nadia, Chang, Ying, Li, Zongjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867652/
https://www.ncbi.nlm.nih.gov/pubmed/35209908
http://dx.doi.org/10.1186/s12951-022-01301-3
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author Huang, Haoyan
Chen, Shang
Cheng, Hui
Cao, Jiasong
Du, Wei
Zhang, Jun
Chang, Yuqiao
Shen, Xiaohong
Guo, Zhikun
Han, Zhibo
Hua, Guoqiang
Han, Zhong-Chao
Benkirane-Jessel, Nadia
Chang, Ying
Li, Zongjin
author_facet Huang, Haoyan
Chen, Shang
Cheng, Hui
Cao, Jiasong
Du, Wei
Zhang, Jun
Chang, Yuqiao
Shen, Xiaohong
Guo, Zhikun
Han, Zhibo
Hua, Guoqiang
Han, Zhong-Chao
Benkirane-Jessel, Nadia
Chang, Ying
Li, Zongjin
author_sort Huang, Haoyan
collection PubMed
description BACKGROUND: The promising therapeutic strategy for the treatment of peripheral artery disease (PAD) is to restore blood supply and promote regeneration of skeletal muscle regeneration. Increasing evidence revealed that prostaglandin E(2) (PGE(2)), a lipid signaling molecule, has significant therapeutic potential for tissue repair and regeneration. Though PGE(2) has been well reported in tissue regeneration, the application of PGE(2) is hampered by its short half-life in vivo and the lack of a viable system for sustained release of PGE(2). RESULTS: In this study, we designed and synthesized a new PGE(2) release matrix by chemically bonding PGE(2) to collagen. Our results revealed that the PGE(2) matrix effectively extends the half-life of PGE(2) in vitro and in vivo. Moreover, the PGE(2) matrix markedly improved neovascularization by increasing angiogenesis, as confirmed by bioluminescence imaging (BLI). Furthermore, the PGE(2) matrix exhibits superior therapeutic efficacy in the hindlimb ischemia model through the activation of MyoD1-mediated muscle stem cells, which is consistent with accelerated structural recovery of skeletal muscle, as evidenced by histological analysis. CONCLUSIONS: Our findings highlight the chemical bonding strategy of chemical bonding PGE(2) to collagen for sustained release and may facilitate the development of PGE(2)-based therapies to significantly improve tissue regeneration. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01301-3.
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spelling pubmed-88676522022-02-28 The sustained PGE(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model Huang, Haoyan Chen, Shang Cheng, Hui Cao, Jiasong Du, Wei Zhang, Jun Chang, Yuqiao Shen, Xiaohong Guo, Zhikun Han, Zhibo Hua, Guoqiang Han, Zhong-Chao Benkirane-Jessel, Nadia Chang, Ying Li, Zongjin J Nanobiotechnology Research BACKGROUND: The promising therapeutic strategy for the treatment of peripheral artery disease (PAD) is to restore blood supply and promote regeneration of skeletal muscle regeneration. Increasing evidence revealed that prostaglandin E(2) (PGE(2)), a lipid signaling molecule, has significant therapeutic potential for tissue repair and regeneration. Though PGE(2) has been well reported in tissue regeneration, the application of PGE(2) is hampered by its short half-life in vivo and the lack of a viable system for sustained release of PGE(2). RESULTS: In this study, we designed and synthesized a new PGE(2) release matrix by chemically bonding PGE(2) to collagen. Our results revealed that the PGE(2) matrix effectively extends the half-life of PGE(2) in vitro and in vivo. Moreover, the PGE(2) matrix markedly improved neovascularization by increasing angiogenesis, as confirmed by bioluminescence imaging (BLI). Furthermore, the PGE(2) matrix exhibits superior therapeutic efficacy in the hindlimb ischemia model through the activation of MyoD1-mediated muscle stem cells, which is consistent with accelerated structural recovery of skeletal muscle, as evidenced by histological analysis. CONCLUSIONS: Our findings highlight the chemical bonding strategy of chemical bonding PGE(2) to collagen for sustained release and may facilitate the development of PGE(2)-based therapies to significantly improve tissue regeneration. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01301-3. BioMed Central 2022-02-24 /pmc/articles/PMC8867652/ /pubmed/35209908 http://dx.doi.org/10.1186/s12951-022-01301-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Huang, Haoyan
Chen, Shang
Cheng, Hui
Cao, Jiasong
Du, Wei
Zhang, Jun
Chang, Yuqiao
Shen, Xiaohong
Guo, Zhikun
Han, Zhibo
Hua, Guoqiang
Han, Zhong-Chao
Benkirane-Jessel, Nadia
Chang, Ying
Li, Zongjin
The sustained PGE(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model
title The sustained PGE(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model
title_full The sustained PGE(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model
title_fullStr The sustained PGE(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model
title_full_unstemmed The sustained PGE(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model
title_short The sustained PGE(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model
title_sort sustained pge(2) release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867652/
https://www.ncbi.nlm.nih.gov/pubmed/35209908
http://dx.doi.org/10.1186/s12951-022-01301-3
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