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Multiple evolutionary origins of glyphosate resistance in Lolium multiflorum

The multitude of herbicide resistance patterns that have evolved in different weed species is a remarkable example of the rapid adaptation to anthropogenic‐driven disturbance. Recently, resistance to glyphosate was identified in multiple populations of Lolium multiflorum in Oregon. We used phenotypi...

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Autores principales: Brunharo, Caio A. C. G., Streisfeld, Matthew A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867705/
https://www.ncbi.nlm.nih.gov/pubmed/35233250
http://dx.doi.org/10.1111/eva.13344
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author Brunharo, Caio A. C. G.
Streisfeld, Matthew A.
author_facet Brunharo, Caio A. C. G.
Streisfeld, Matthew A.
author_sort Brunharo, Caio A. C. G.
collection PubMed
description The multitude of herbicide resistance patterns that have evolved in different weed species is a remarkable example of the rapid adaptation to anthropogenic‐driven disturbance. Recently, resistance to glyphosate was identified in multiple populations of Lolium multiflorum in Oregon. We used phenotypic approaches, as well as population genomic and gene expression analyses, to determine whether known mechanisms were responsible for glyphosate resistance and whether resistance phenotypes evolved independently in different populations, and to identify potential loci contributing to resistance. We found no evidence of genetic alterations or expression changes at known target and non‐target‐site resistance mechanisms of glyphosate. Population genomic analyses indicated that resistant populations tended to have largely distinct ancestry from one another, suggesting that glyphosate resistance did not spread among populations by gene flow. Rather, resistance appears to have evolved independently on different genetic backgrounds. We also detected potential loci associated with the resistance phenotype, some of which encode proteins with potential effects on herbicide metabolism. Our results suggest that Oregon populations of L. multiflorum evolved resistance to glyphosate due to a novel mechanism. Future studies that characterize the gene or genes involved in resistance will be necessary to confirm this conclusion.
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spelling pubmed-88677052022-02-28 Multiple evolutionary origins of glyphosate resistance in Lolium multiflorum Brunharo, Caio A. C. G. Streisfeld, Matthew A. Evol Appl Original Articles The multitude of herbicide resistance patterns that have evolved in different weed species is a remarkable example of the rapid adaptation to anthropogenic‐driven disturbance. Recently, resistance to glyphosate was identified in multiple populations of Lolium multiflorum in Oregon. We used phenotypic approaches, as well as population genomic and gene expression analyses, to determine whether known mechanisms were responsible for glyphosate resistance and whether resistance phenotypes evolved independently in different populations, and to identify potential loci contributing to resistance. We found no evidence of genetic alterations or expression changes at known target and non‐target‐site resistance mechanisms of glyphosate. Population genomic analyses indicated that resistant populations tended to have largely distinct ancestry from one another, suggesting that glyphosate resistance did not spread among populations by gene flow. Rather, resistance appears to have evolved independently on different genetic backgrounds. We also detected potential loci associated with the resistance phenotype, some of which encode proteins with potential effects on herbicide metabolism. Our results suggest that Oregon populations of L. multiflorum evolved resistance to glyphosate due to a novel mechanism. Future studies that characterize the gene or genes involved in resistance will be necessary to confirm this conclusion. John Wiley and Sons Inc. 2022-02-08 /pmc/articles/PMC8867705/ /pubmed/35233250 http://dx.doi.org/10.1111/eva.13344 Text en © 2022 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Brunharo, Caio A. C. G.
Streisfeld, Matthew A.
Multiple evolutionary origins of glyphosate resistance in Lolium multiflorum
title Multiple evolutionary origins of glyphosate resistance in Lolium multiflorum
title_full Multiple evolutionary origins of glyphosate resistance in Lolium multiflorum
title_fullStr Multiple evolutionary origins of glyphosate resistance in Lolium multiflorum
title_full_unstemmed Multiple evolutionary origins of glyphosate resistance in Lolium multiflorum
title_short Multiple evolutionary origins of glyphosate resistance in Lolium multiflorum
title_sort multiple evolutionary origins of glyphosate resistance in lolium multiflorum
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867705/
https://www.ncbi.nlm.nih.gov/pubmed/35233250
http://dx.doi.org/10.1111/eva.13344
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