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Paper-Based Progesterone Sensor Using an Allosteric Transcription Factor
[Image: see text] Progesterone monitoring is an essential component of in vitro fertilization treatments and reproductive management of dairy cows. Gold-standard biosensors for progesterone monitoring rely on antibodies, which are expensive and difficult to procure. We have developed an alternative...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867790/ https://www.ncbi.nlm.nih.gov/pubmed/35224340 http://dx.doi.org/10.1021/acsomega.1c05737 |
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author | Zamani, Marjon Dupaty, Josh Baer, R. C. Kuzmanovic, Uros Fan, Andy Grinstaff, Mark W. Galagan, James E. Klapperich, Catherine M. |
author_facet | Zamani, Marjon Dupaty, Josh Baer, R. C. Kuzmanovic, Uros Fan, Andy Grinstaff, Mark W. Galagan, James E. Klapperich, Catherine M. |
author_sort | Zamani, Marjon |
collection | PubMed |
description | [Image: see text] Progesterone monitoring is an essential component of in vitro fertilization treatments and reproductive management of dairy cows. Gold-standard biosensors for progesterone monitoring rely on antibodies, which are expensive and difficult to procure. We have developed an alternative transcription factor-based sensor that is superior to conventional progesterone biosensors. Here, we incorporate this transcription factor-based progesterone sensor into an affordable, portable paperfluidic format to facilitate widespread implementation of progesterone monitoring at the point of care. Oligonucleotides labeled with a fluorescent dye are immobilized onto nitrocellulose via a biotin–streptavidin interaction. In the absence of progesterone, these oligonucleotides form a complex with a transcription factor that is fluorescently labeled with tdTomato. In the presence of progesterone, the fluorescent transcription factor unbinds from the immobilized DNA, resulting in a decrease in tdTomato fluorescence. The limit of detection of our system is 27 nm, which is a clinically relevant level of progesterone. We demonstrate that transcription factor-based sensors can be incorporated into paperfluidic devices, thereby making them accessible to a broader population due to the portability and affordability of paper-based devices. |
format | Online Article Text |
id | pubmed-8867790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-88677902022-02-25 Paper-Based Progesterone Sensor Using an Allosteric Transcription Factor Zamani, Marjon Dupaty, Josh Baer, R. C. Kuzmanovic, Uros Fan, Andy Grinstaff, Mark W. Galagan, James E. Klapperich, Catherine M. ACS Omega [Image: see text] Progesterone monitoring is an essential component of in vitro fertilization treatments and reproductive management of dairy cows. Gold-standard biosensors for progesterone monitoring rely on antibodies, which are expensive and difficult to procure. We have developed an alternative transcription factor-based sensor that is superior to conventional progesterone biosensors. Here, we incorporate this transcription factor-based progesterone sensor into an affordable, portable paperfluidic format to facilitate widespread implementation of progesterone monitoring at the point of care. Oligonucleotides labeled with a fluorescent dye are immobilized onto nitrocellulose via a biotin–streptavidin interaction. In the absence of progesterone, these oligonucleotides form a complex with a transcription factor that is fluorescently labeled with tdTomato. In the presence of progesterone, the fluorescent transcription factor unbinds from the immobilized DNA, resulting in a decrease in tdTomato fluorescence. The limit of detection of our system is 27 nm, which is a clinically relevant level of progesterone. We demonstrate that transcription factor-based sensors can be incorporated into paperfluidic devices, thereby making them accessible to a broader population due to the portability and affordability of paper-based devices. American Chemical Society 2022-02-07 /pmc/articles/PMC8867790/ /pubmed/35224340 http://dx.doi.org/10.1021/acsomega.1c05737 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Zamani, Marjon Dupaty, Josh Baer, R. C. Kuzmanovic, Uros Fan, Andy Grinstaff, Mark W. Galagan, James E. Klapperich, Catherine M. Paper-Based Progesterone Sensor Using an Allosteric Transcription Factor |
title | Paper-Based Progesterone Sensor Using an Allosteric
Transcription Factor |
title_full | Paper-Based Progesterone Sensor Using an Allosteric
Transcription Factor |
title_fullStr | Paper-Based Progesterone Sensor Using an Allosteric
Transcription Factor |
title_full_unstemmed | Paper-Based Progesterone Sensor Using an Allosteric
Transcription Factor |
title_short | Paper-Based Progesterone Sensor Using an Allosteric
Transcription Factor |
title_sort | paper-based progesterone sensor using an allosteric
transcription factor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867790/ https://www.ncbi.nlm.nih.gov/pubmed/35224340 http://dx.doi.org/10.1021/acsomega.1c05737 |
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