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MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway
Preeclampsia (PE), a pregnancy disorder that affects 5–7% of pregnant women, is among the primary causes for maternal and perinatal mortality. PE is believed to be associated with insufficient invasion of villous and extravillous trophoblasts (EVTs), which hampers uterine spiral artery remodeling an...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867866/ https://www.ncbi.nlm.nih.gov/pubmed/35209928 http://dx.doi.org/10.1186/s12958-022-00909-2 |
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author | Zang, Junjie Yan, Min Zhang, Yan Peng, Wei Zuo, Jianxin Zhou, Huansheng Gao, Guoqiang Li, Min Chu, Yijing Ye, Yuanhua |
author_facet | Zang, Junjie Yan, Min Zhang, Yan Peng, Wei Zuo, Jianxin Zhou, Huansheng Gao, Guoqiang Li, Min Chu, Yijing Ye, Yuanhua |
author_sort | Zang, Junjie |
collection | PubMed |
description | Preeclampsia (PE), a pregnancy disorder that affects 5–7% of pregnant women, is among the primary causes for maternal and perinatal mortality. PE is believed to be associated with insufficient invasion of villous and extravillous trophoblasts (EVTs), which hampers uterine spiral artery remodeling and finally induces PE. But the mechanism responsible for reduction of trophoblast invasion remains unclear. In this study, placental tissues taken from healthy donors and PE patients were used to evaluate the miR-326 expression; CCK8 and colony formation assays were used to confirm the effect of miR-326 on cell proliferation; transwell assay was used to demonstrate the effect of miR-326 on cell invasion capability; western blot was used to investigate the underlying mechanism; and luciferase assay was used to detect the effect of miR-326 on YAP/TAZ-mediated transcription activity. It was revealed the miR-326 expression was higher in placentas from PE patients than from healthy donors. After transfection of miR-326 mimics, trophoblast proliferation and invasion were impaired. Using TargetScan, we speculated that PAX8 was a target of miR-326, which was later confirmed by western blot. The YAP/TAZ expression was also downregulated after transfection with miR-326. Luciferase assay demonstrated that overexpression of miR-326 suppressed YAP/TAZ-mediated transcription activity by targeting PAX8. Overexpression of PAX8 could partly rescue miR-326-induced suppression of trophoblast proliferation and invasion. Taken together, our result indicated that miR-326 suppresses trophoblast growth, invasion, and migration by means of targeting PAX8 via the Hippo pathway. |
format | Online Article Text |
id | pubmed-8867866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-88678662022-02-25 MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway Zang, Junjie Yan, Min Zhang, Yan Peng, Wei Zuo, Jianxin Zhou, Huansheng Gao, Guoqiang Li, Min Chu, Yijing Ye, Yuanhua Reprod Biol Endocrinol Research Preeclampsia (PE), a pregnancy disorder that affects 5–7% of pregnant women, is among the primary causes for maternal and perinatal mortality. PE is believed to be associated with insufficient invasion of villous and extravillous trophoblasts (EVTs), which hampers uterine spiral artery remodeling and finally induces PE. But the mechanism responsible for reduction of trophoblast invasion remains unclear. In this study, placental tissues taken from healthy donors and PE patients were used to evaluate the miR-326 expression; CCK8 and colony formation assays were used to confirm the effect of miR-326 on cell proliferation; transwell assay was used to demonstrate the effect of miR-326 on cell invasion capability; western blot was used to investigate the underlying mechanism; and luciferase assay was used to detect the effect of miR-326 on YAP/TAZ-mediated transcription activity. It was revealed the miR-326 expression was higher in placentas from PE patients than from healthy donors. After transfection of miR-326 mimics, trophoblast proliferation and invasion were impaired. Using TargetScan, we speculated that PAX8 was a target of miR-326, which was later confirmed by western blot. The YAP/TAZ expression was also downregulated after transfection with miR-326. Luciferase assay demonstrated that overexpression of miR-326 suppressed YAP/TAZ-mediated transcription activity by targeting PAX8. Overexpression of PAX8 could partly rescue miR-326-induced suppression of trophoblast proliferation and invasion. Taken together, our result indicated that miR-326 suppresses trophoblast growth, invasion, and migration by means of targeting PAX8 via the Hippo pathway. BioMed Central 2022-02-24 /pmc/articles/PMC8867866/ /pubmed/35209928 http://dx.doi.org/10.1186/s12958-022-00909-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zang, Junjie Yan, Min Zhang, Yan Peng, Wei Zuo, Jianxin Zhou, Huansheng Gao, Guoqiang Li, Min Chu, Yijing Ye, Yuanhua MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway |
title | MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway |
title_full | MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway |
title_fullStr | MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway |
title_full_unstemmed | MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway |
title_short | MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway |
title_sort | mir-326 inhibits trophoblast growth, migration, and invasion by targeting pax8 via hippo pathway |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867866/ https://www.ncbi.nlm.nih.gov/pubmed/35209928 http://dx.doi.org/10.1186/s12958-022-00909-2 |
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