Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay

Early detection of expanded‐spectrum cephalosporin (ESC) resistance is essential not only for an effective therapy but also for the prompt implementation of infection control measures to prevent dissemination in the hospital. We have developed and validated a lateral flow immunoassay (LFIA), called...

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Autores principales: Moguet, Christian, Gonzalez, Camille, Sallustrau, Antoine, Gelhaye, Stéphanie, Naas, Thierry, Simon, Stéphanie, Volland, Hervé
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867991/
https://www.ncbi.nlm.nih.gov/pubmed/34342151
http://dx.doi.org/10.1111/1751-7915.13892
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author Moguet, Christian
Gonzalez, Camille
Sallustrau, Antoine
Gelhaye, Stéphanie
Naas, Thierry
Simon, Stéphanie
Volland, Hervé
author_facet Moguet, Christian
Gonzalez, Camille
Sallustrau, Antoine
Gelhaye, Stéphanie
Naas, Thierry
Simon, Stéphanie
Volland, Hervé
author_sort Moguet, Christian
collection PubMed
description Early detection of expanded‐spectrum cephalosporin (ESC) resistance is essential not only for an effective therapy but also for the prompt implementation of infection control measures to prevent dissemination in the hospital. We have developed and validated a lateral flow immunoassay (LFIA), called LFIA‐CTX test, for the detection of ESC (cefotaxime) hydrolytic activity based on structural discrimination between the intact antibiotic and its hydrolysed product. A single bacterial colony was suspended in an extraction buffer containing cefotaxime. After a 30‐min incubation, the solution is loaded on the LFIA for reading within 10 min. A total of 348 well‐characterized Gram‐negative isolates were tested. Among them, the 38 isolates that did not express any cefotaxime‐hydrolysing β‐lactamase gave negative results. Of the 310 isolates expressing at least one cefotaxime‐hydrolysing β‐lactamase, all were tested positive, except three OXA‐48‐like producers, which were repeatedly detected negative. Therefore, the sensitivity was 99.1% and the specificity was 100%. The LFIA‐CTX test is efficient, fast, low‐cost and easy to implement in the workflow of a routine microbiology laboratory.
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spelling pubmed-88679912022-02-28 Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay Moguet, Christian Gonzalez, Camille Sallustrau, Antoine Gelhaye, Stéphanie Naas, Thierry Simon, Stéphanie Volland, Hervé Microb Biotechnol Research Articles Early detection of expanded‐spectrum cephalosporin (ESC) resistance is essential not only for an effective therapy but also for the prompt implementation of infection control measures to prevent dissemination in the hospital. We have developed and validated a lateral flow immunoassay (LFIA), called LFIA‐CTX test, for the detection of ESC (cefotaxime) hydrolytic activity based on structural discrimination between the intact antibiotic and its hydrolysed product. A single bacterial colony was suspended in an extraction buffer containing cefotaxime. After a 30‐min incubation, the solution is loaded on the LFIA for reading within 10 min. A total of 348 well‐characterized Gram‐negative isolates were tested. Among them, the 38 isolates that did not express any cefotaxime‐hydrolysing β‐lactamase gave negative results. Of the 310 isolates expressing at least one cefotaxime‐hydrolysing β‐lactamase, all were tested positive, except three OXA‐48‐like producers, which were repeatedly detected negative. Therefore, the sensitivity was 99.1% and the specificity was 100%. The LFIA‐CTX test is efficient, fast, low‐cost and easy to implement in the workflow of a routine microbiology laboratory. John Wiley and Sons Inc. 2021-08-02 /pmc/articles/PMC8867991/ /pubmed/34342151 http://dx.doi.org/10.1111/1751-7915.13892 Text en © 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Moguet, Christian
Gonzalez, Camille
Sallustrau, Antoine
Gelhaye, Stéphanie
Naas, Thierry
Simon, Stéphanie
Volland, Hervé
Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay
title Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay
title_full Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay
title_fullStr Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay
title_full_unstemmed Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay
title_short Detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay
title_sort detection of expanded‐spectrum cephalosporin hydrolysis by lateral flow immunoassay
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8867991/
https://www.ncbi.nlm.nih.gov/pubmed/34342151
http://dx.doi.org/10.1111/1751-7915.13892
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