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Genome-wide identification and characterization of laccase family members in eggplant (Solanum melongena L.)

Laccase, as a copper-containing polyphenol oxidase, primarily functions in the process of lignin, anthocyanin biosynthesis, and various abiotic/biotic stresses. In this study, forty-eight laccase members were identified in the eggplant genome. Only forty-two laccase genes from eggplant (SmLACs) were...

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Autores principales: Wan, Faxiang, Zhang, Linqing, Tan, Mengying, Wang, Xiaohua, Wang, Guang-Long, Qi, Mengru, Liu, Bingxin, Gao, Jun, Pan, Yu, Wang, Yongqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868016/
https://www.ncbi.nlm.nih.gov/pubmed/35223206
http://dx.doi.org/10.7717/peerj.12922
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author Wan, Faxiang
Zhang, Linqing
Tan, Mengying
Wang, Xiaohua
Wang, Guang-Long
Qi, Mengru
Liu, Bingxin
Gao, Jun
Pan, Yu
Wang, Yongqing
author_facet Wan, Faxiang
Zhang, Linqing
Tan, Mengying
Wang, Xiaohua
Wang, Guang-Long
Qi, Mengru
Liu, Bingxin
Gao, Jun
Pan, Yu
Wang, Yongqing
author_sort Wan, Faxiang
collection PubMed
description Laccase, as a copper-containing polyphenol oxidase, primarily functions in the process of lignin, anthocyanin biosynthesis, and various abiotic/biotic stresses. In this study, forty-eight laccase members were identified in the eggplant genome. Only forty-two laccase genes from eggplant (SmLACs) were anchored unevenly in 12 chromosomes, the other six SmLACs were mapped on unanchored scaffolds. Phylogenetic analysis indicated that only twenty-five SmLACs were divided into six different groups on the basis of groups reported in Arabidopsis. Gene structure analysis revealed that the number of exons ranged from one to 13. Motif analysis revealed that SmLACs included six conserved motifs. In aspects of gene duplication analysis, twenty-one SmLACs were collinear with LAC genes from Arabidopsis, tomato or rice. Cis-regulatory elements analysis indicated many SmLACs may be involved in eggplant morphogenesis, flavonoid biosynthesis, diverse stresses and growth/development processes. Expression analysis further confirmed that a few SmLACs may function in vegetative and reproductive organs at different developmental stages and also in response to one or multiple stresses. This study would help to further understand and enrich the physiological function of the SmLAC gene family in eggplant, and may provide high-quality genetic resources for eggplant genetics and breeding.
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spelling pubmed-88680162022-02-25 Genome-wide identification and characterization of laccase family members in eggplant (Solanum melongena L.) Wan, Faxiang Zhang, Linqing Tan, Mengying Wang, Xiaohua Wang, Guang-Long Qi, Mengru Liu, Bingxin Gao, Jun Pan, Yu Wang, Yongqing PeerJ Agricultural Science Laccase, as a copper-containing polyphenol oxidase, primarily functions in the process of lignin, anthocyanin biosynthesis, and various abiotic/biotic stresses. In this study, forty-eight laccase members were identified in the eggplant genome. Only forty-two laccase genes from eggplant (SmLACs) were anchored unevenly in 12 chromosomes, the other six SmLACs were mapped on unanchored scaffolds. Phylogenetic analysis indicated that only twenty-five SmLACs were divided into six different groups on the basis of groups reported in Arabidopsis. Gene structure analysis revealed that the number of exons ranged from one to 13. Motif analysis revealed that SmLACs included six conserved motifs. In aspects of gene duplication analysis, twenty-one SmLACs were collinear with LAC genes from Arabidopsis, tomato or rice. Cis-regulatory elements analysis indicated many SmLACs may be involved in eggplant morphogenesis, flavonoid biosynthesis, diverse stresses and growth/development processes. Expression analysis further confirmed that a few SmLACs may function in vegetative and reproductive organs at different developmental stages and also in response to one or multiple stresses. This study would help to further understand and enrich the physiological function of the SmLAC gene family in eggplant, and may provide high-quality genetic resources for eggplant genetics and breeding. PeerJ Inc. 2022-02-21 /pmc/articles/PMC8868016/ /pubmed/35223206 http://dx.doi.org/10.7717/peerj.12922 Text en © 2022 Wan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Wan, Faxiang
Zhang, Linqing
Tan, Mengying
Wang, Xiaohua
Wang, Guang-Long
Qi, Mengru
Liu, Bingxin
Gao, Jun
Pan, Yu
Wang, Yongqing
Genome-wide identification and characterization of laccase family members in eggplant (Solanum melongena L.)
title Genome-wide identification and characterization of laccase family members in eggplant (Solanum melongena L.)
title_full Genome-wide identification and characterization of laccase family members in eggplant (Solanum melongena L.)
title_fullStr Genome-wide identification and characterization of laccase family members in eggplant (Solanum melongena L.)
title_full_unstemmed Genome-wide identification and characterization of laccase family members in eggplant (Solanum melongena L.)
title_short Genome-wide identification and characterization of laccase family members in eggplant (Solanum melongena L.)
title_sort genome-wide identification and characterization of laccase family members in eggplant (solanum melongena l.)
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868016/
https://www.ncbi.nlm.nih.gov/pubmed/35223206
http://dx.doi.org/10.7717/peerj.12922
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