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Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life

The association between prolonged antibiotic (AB) use in neonates and increased incidence of later life diseases is not yet fully understood. AB treatment in early life alters intestinal epithelial cell composition, functioning, and maturation, which could be the basis for later life health effects....

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Autores principales: Martins Garcia, Tânia, van Roest, Manon, Vermeulen, Jacqueline L. M., Meisner, Sander, Koster, Jan, Wildenberg, Manon E., van Elburg, Ruurd M., Muncan, Vanesa, Renes, Ingrid B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868095/
https://www.ncbi.nlm.nih.gov/pubmed/35203869
http://dx.doi.org/10.3390/antibiotics11020267
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author Martins Garcia, Tânia
van Roest, Manon
Vermeulen, Jacqueline L. M.
Meisner, Sander
Koster, Jan
Wildenberg, Manon E.
van Elburg, Ruurd M.
Muncan, Vanesa
Renes, Ingrid B.
author_facet Martins Garcia, Tânia
van Roest, Manon
Vermeulen, Jacqueline L. M.
Meisner, Sander
Koster, Jan
Wildenberg, Manon E.
van Elburg, Ruurd M.
Muncan, Vanesa
Renes, Ingrid B.
author_sort Martins Garcia, Tânia
collection PubMed
description The association between prolonged antibiotic (AB) use in neonates and increased incidence of later life diseases is not yet fully understood. AB treatment in early life alters intestinal epithelial cell composition, functioning, and maturation, which could be the basis for later life health effects. Here, we investigated whether AB-induced changes in the neonatal gut persisted up to adulthood and whether early life AB had additional long-term consequences for gut functioning. Mice received AB orally from postnatal day 10 to 20. Intestinal morphology, permeability, and gene and protein expression at 8 weeks were analyzed. Our data showed that the majority of the early life AB-induced gut effects did not persist into adulthood, yet early life AB did impact later life gut functioning. Specifically, the proximal small intestine (SI) of adult mice treated with AB in early life was characterized by hyperproliferative crypts, increased number of Paneth cells, and alterations in enteroendocrine cell-specific gene expression profiles. The distal SI of adult mice displayed a reduced expression of antibacterial defense markers. Together, our results suggest that early life AB leads to structural and physiological changes in the adult gut, which may contribute to disease development when homeostatic conditions are under challenge.
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spelling pubmed-88680952022-02-25 Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life Martins Garcia, Tânia van Roest, Manon Vermeulen, Jacqueline L. M. Meisner, Sander Koster, Jan Wildenberg, Manon E. van Elburg, Ruurd M. Muncan, Vanesa Renes, Ingrid B. Antibiotics (Basel) Article The association between prolonged antibiotic (AB) use in neonates and increased incidence of later life diseases is not yet fully understood. AB treatment in early life alters intestinal epithelial cell composition, functioning, and maturation, which could be the basis for later life health effects. Here, we investigated whether AB-induced changes in the neonatal gut persisted up to adulthood and whether early life AB had additional long-term consequences for gut functioning. Mice received AB orally from postnatal day 10 to 20. Intestinal morphology, permeability, and gene and protein expression at 8 weeks were analyzed. Our data showed that the majority of the early life AB-induced gut effects did not persist into adulthood, yet early life AB did impact later life gut functioning. Specifically, the proximal small intestine (SI) of adult mice treated with AB in early life was characterized by hyperproliferative crypts, increased number of Paneth cells, and alterations in enteroendocrine cell-specific gene expression profiles. The distal SI of adult mice displayed a reduced expression of antibacterial defense markers. Together, our results suggest that early life AB leads to structural and physiological changes in the adult gut, which may contribute to disease development when homeostatic conditions are under challenge. MDPI 2022-02-18 /pmc/articles/PMC8868095/ /pubmed/35203869 http://dx.doi.org/10.3390/antibiotics11020267 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martins Garcia, Tânia
van Roest, Manon
Vermeulen, Jacqueline L. M.
Meisner, Sander
Koster, Jan
Wildenberg, Manon E.
van Elburg, Ruurd M.
Muncan, Vanesa
Renes, Ingrid B.
Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life
title Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life
title_full Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life
title_fullStr Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life
title_full_unstemmed Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life
title_short Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life
title_sort altered gut structure and anti-bacterial defense in adult mice treated with antibiotics during early life
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868095/
https://www.ncbi.nlm.nih.gov/pubmed/35203869
http://dx.doi.org/10.3390/antibiotics11020267
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