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Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae

There are real concerns that Neisseria gonorrhoeae may become untreatable in the near future due to the rapid emergence of antimicrobial resistance. Alternative therapies are thus urgently required. Bacteriophages active against N. gonorrhoeae could play an important role as an antibiotic-sparing th...

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Autores principales: Laumen, Jolein Gyonne Elise, Abdellati, Saïd, Manoharan-Basil, Sheeba Santhini, Van Dijck, Christophe, Van den Bossche, Dorien, De Baetselier, Irith, de Block, Tessa, Malhotra-Kumar, Surbhi, Soentjes, Patrick, Pirnay, Jean-Paul, Kenyon, Chris, Merabishvili, Maia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868155/
https://www.ncbi.nlm.nih.gov/pubmed/35203870
http://dx.doi.org/10.3390/antibiotics11020268
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author Laumen, Jolein Gyonne Elise
Abdellati, Saïd
Manoharan-Basil, Sheeba Santhini
Van Dijck, Christophe
Van den Bossche, Dorien
De Baetselier, Irith
de Block, Tessa
Malhotra-Kumar, Surbhi
Soentjes, Patrick
Pirnay, Jean-Paul
Kenyon, Chris
Merabishvili, Maia
author_facet Laumen, Jolein Gyonne Elise
Abdellati, Saïd
Manoharan-Basil, Sheeba Santhini
Van Dijck, Christophe
Van den Bossche, Dorien
De Baetselier, Irith
de Block, Tessa
Malhotra-Kumar, Surbhi
Soentjes, Patrick
Pirnay, Jean-Paul
Kenyon, Chris
Merabishvili, Maia
author_sort Laumen, Jolein Gyonne Elise
collection PubMed
description There are real concerns that Neisseria gonorrhoeae may become untreatable in the near future due to the rapid emergence of antimicrobial resistance. Alternative therapies are thus urgently required. Bacteriophages active against N. gonorrhoeae could play an important role as an antibiotic-sparing therapy. To the best of our knowledge, no bacteriophages active against N. gonorrhoeae have ever been found. The aim of this study was to screen for bacteriophages able to lyse N. gonorrhoeae in oropharyngeal and anorectal swabs of 74 men who have sex with men attending a sexual health clinic in Antwerp, Belgium. We screened 210 swabs but were unable to identify an anti-gonococcal bacteriophage. This is the first report of a pilot screening that systematically searched for anti-gonococcal phages directly from clinical swabs. Further studies may consider screening for phages at other anatomical sites (e.g., stool samples, urine) or in environmental settings (e.g., toilet sewage water of sex clubs or sexually transmitted infection clinics) where N. gonorrhoeae can be found.
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spelling pubmed-88681552022-02-25 Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae Laumen, Jolein Gyonne Elise Abdellati, Saïd Manoharan-Basil, Sheeba Santhini Van Dijck, Christophe Van den Bossche, Dorien De Baetselier, Irith de Block, Tessa Malhotra-Kumar, Surbhi Soentjes, Patrick Pirnay, Jean-Paul Kenyon, Chris Merabishvili, Maia Antibiotics (Basel) Article There are real concerns that Neisseria gonorrhoeae may become untreatable in the near future due to the rapid emergence of antimicrobial resistance. Alternative therapies are thus urgently required. Bacteriophages active against N. gonorrhoeae could play an important role as an antibiotic-sparing therapy. To the best of our knowledge, no bacteriophages active against N. gonorrhoeae have ever been found. The aim of this study was to screen for bacteriophages able to lyse N. gonorrhoeae in oropharyngeal and anorectal swabs of 74 men who have sex with men attending a sexual health clinic in Antwerp, Belgium. We screened 210 swabs but were unable to identify an anti-gonococcal bacteriophage. This is the first report of a pilot screening that systematically searched for anti-gonococcal phages directly from clinical swabs. Further studies may consider screening for phages at other anatomical sites (e.g., stool samples, urine) or in environmental settings (e.g., toilet sewage water of sex clubs or sexually transmitted infection clinics) where N. gonorrhoeae can be found. MDPI 2022-02-18 /pmc/articles/PMC8868155/ /pubmed/35203870 http://dx.doi.org/10.3390/antibiotics11020268 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Laumen, Jolein Gyonne Elise
Abdellati, Saïd
Manoharan-Basil, Sheeba Santhini
Van Dijck, Christophe
Van den Bossche, Dorien
De Baetselier, Irith
de Block, Tessa
Malhotra-Kumar, Surbhi
Soentjes, Patrick
Pirnay, Jean-Paul
Kenyon, Chris
Merabishvili, Maia
Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae
title Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae
title_full Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae
title_fullStr Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae
title_full_unstemmed Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae
title_short Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae
title_sort screening of anorectal and oropharyngeal samples fails to detect bacteriophages infecting neisseria gonorrhoeae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868155/
https://www.ncbi.nlm.nih.gov/pubmed/35203870
http://dx.doi.org/10.3390/antibiotics11020268
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