Evaluation of Stress Accompanying Immunocontraceptive Vaccination in Donkeys

SIMPLE SUMMARY: The overpopulation of donkeys is of concern worldwide. Some of the concerns associated with uncontrolled donkey populations are the destruction of habitat, competition for feed resources, and negative human–animal interactions. One of the most effective and humane solutions is the use of immunocontraception. Immunocontraception is the use of an animal’s immune system to prevent it from fertilizing offspring by the administration of a vaccine that targets a specific portion of the reproductive system. This study sought to measure and compare the amount of stress that is imposed when two different immunocontraceptives were administered to female donkeys. The results showed that physical exam parameters (temperature, pulse, and respiration) were not affected significantly but the measurement of stress hormones suggests an acute stress response after the first vaccination that was followed by a chronic stress response after a booster vaccination 35 days later, especially in donkeys that had reactions at the injection site. Further studies would be required to identify and reduce the stress associated with the immunocontraception vaccines that were used. ABSTRACT: The overpopulation of donkeys is recognized as a problem in many parts of the world. The main concerns with uncontrolled donkey populations are habitat degradation and competition for feed resources between donkeys and other species. One of the most effective and humane solutions is the use of immunocontraception. Therefore, this study sought to evaluate the stress imposed by the use of two formulations of a zona pellucida (ZP) vaccine, a recombinant (reZP) and a native porcine (pZP) vaccine, both formulated with a Freund’s adjuvant. The stress was objectively measured using fecal cortisol concentrations and physical examination parameters at fixed points before and after vaccination. We hypothesized that fewer changes in physical exam parameters and lower fecal cortisol concentrations would be stimulated in jennies treated with the reZP vaccine due to the selection of specific proteins. Twenty-five reproductively sound jennies were randomly assigned to reZP (n = 9), pZP (n = 8) or control (n = 8) groups. The vaccines were administered at five-week intervals. Physical exam parameters and body wall thickness of injection sites were recorded for each jenny for four days post-injections. Fecal samples were obtained every other day from day 0 (first vaccination) through day 6 and on days 35 to 41 after booster. Injection site reactions were common in all groups with the reZP and pZP groups being overrepresented. Lameness was observed in the pZP and reZP groups that were affected by injection site reactions and open abscesses. The present study showed an increase in fecal cortisol concentrations within 4 days after the first vaccination with ZP vaccines and, thereafter, a decrease in cortisol 35 days later after the second vaccination, especially in donkeys with open abscesses. Our results suggest that acute stress (increased cortisol) was induced after the first vaccination, and chronic stress (decreased cortisol) occurred thereafter in association with open abscesses. In conclusion, reZP and pZP formulated with Freund’s adjuvant induced local inflammatory reactions with a differential degree of acute and chronic stress in donkeys.