Case Studies in Polar Bear (Ursus maritimus) Sperm Collection and Cryopreservation Techniques

SIMPLE SUMMARY: Polar bears are threatened by habitat loss, decreased food availability, and reduced reproductive success due to climate change. Zoo populations can support species survival through preservation of genetic diversity and maintenance of insurance populations, but in the US, the zoo polar bear population is currently not sustainable. The development of sperm collection and cryopreservation can help to support the population by providing the biomaterial needed for assisted reproductive techniques, such as artificial insemination. However, these procedures are not well described for polar bears. Data from 38 opportunistic sperm collections, that were conducted between 2011 and 2021, were assessed to establish best practices to date for collecting and preserving polar bear sperm. The information gathered demonstrates that urethral catheterization is an efficient method of sperm collection, sperm can be rescued postmortem from the vasa deferentia and epididymides, and polar bear sperm collection appears to be most effective during the breeding season. Furthermore, polar bear sperm can survive cryopreservation. Further studies will optimize these techniques, but this summary provides information that is immediately applicable to enhancing sample collection and cryopreservation success that could support the long-term genetic management of polar bears in zoos. ABSTRACT: Assisted reproductive technologies can aid conservation efforts via support of ex situ population management and preservation of genetic material. Data from 38 sperm collection attempts from 17 polar bears (1–5 procedures/bear) were evaluated. Sample collections were attempted via electroejaculation (EEJ; n = 6), urethral catheterization (UC; n = 25), or sperm rescue (SR; n = 7) during the breeding season (Jan. 1-May 21; n = 27) and nonbreeding season (May 22-Dec. 31; n = 11). Sperm retrieval was successful in 1 EEJ (16.7%), 18 UC (72.0%) and 4 SR (57.1%) collections. Initial sperm motility and viability were 50.0% and 77.0% for EEJ, 64.3 ± 7.4% and 80.9 ± 3.8% for UC, and 56.7 ± 8.8% and 80.5 ± 0.5% for SR. UC and SR were more likely to be successful during the breeding season (84.2–100%) than the nonbreeding season (25.0–33.3%). Testicular tumors were observed in four males (57%) during SR. In total, 13 samples were cryopreserved (n = 1 EEJ, 9 UC, and 3 SR) with egg-yolk-based equine extender (EQ) or OptiXcell (OP). For both extenders, post-thaw motility and viability were reduced by 20–60% and 30–65%, respectively. Further efforts to optimize procedures are warranted, but this summary provides data useful for enhancing the success of polar bear sperm collection and cryopreservation.