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Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile

Bovine embryos are typically cultured at reduced oxygen tension to lower the impact of oxidative stress on embryo development. However, oocyte in vitro maturation (IVM) is performed at atmospheric oxygen tension since low oxygen during maturation has a negative impact on oocyte developmental compete...

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Autores principales: Sidi, Shehu, Pascottini, Osvaldo Bogado, Angel-Velez, Daniel, Azari-Dolatabad, Nima, Pavani, Krishna Chaitanya, Residiwati, Gretania, Meese, Tim, Van Nieuwerburgh, Filip, Kambai Bawa, Elias, Alikidon Voh, Ambrose, Olusegun Ayo, Joseph, Van Soom, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868338/
https://www.ncbi.nlm.nih.gov/pubmed/35204226
http://dx.doi.org/10.3390/antiox11020344
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author Sidi, Shehu
Pascottini, Osvaldo Bogado
Angel-Velez, Daniel
Azari-Dolatabad, Nima
Pavani, Krishna Chaitanya
Residiwati, Gretania
Meese, Tim
Van Nieuwerburgh, Filip
Kambai Bawa, Elias
Alikidon Voh, Ambrose
Olusegun Ayo, Joseph
Van Soom, Ann
author_facet Sidi, Shehu
Pascottini, Osvaldo Bogado
Angel-Velez, Daniel
Azari-Dolatabad, Nima
Pavani, Krishna Chaitanya
Residiwati, Gretania
Meese, Tim
Van Nieuwerburgh, Filip
Kambai Bawa, Elias
Alikidon Voh, Ambrose
Olusegun Ayo, Joseph
Van Soom, Ann
author_sort Sidi, Shehu
collection PubMed
description Bovine embryos are typically cultured at reduced oxygen tension to lower the impact of oxidative stress on embryo development. However, oocyte in vitro maturation (IVM) is performed at atmospheric oxygen tension since low oxygen during maturation has a negative impact on oocyte developmental competence. Lycopene, a carotenoid, acts as a powerful antioxidant and may protect the oocyte against oxidative stress during maturation at atmospheric oxygen conditions. Here, we assessed the effect of adding 0.2 μM lycopene (antioxidant), 5 μM menadione (pro-oxidant), and their combination on the generation of reactive oxygen species (ROS) in matured oocytes and the subsequent development, quality, and transcriptome of the blastocysts in a bovine in vitro model. ROS fluorescent intensity in matured oocytes was significantly lower in the lycopene group, and the resulting embryos showed a significantly higher blastocyst rate on day 8 and a lower apoptotic cell ratio than all other groups. Transcriptomic analysis disclosed a total of 296 differentially expressed genes (Benjamini–Hochberg-adjusted p < 0.05 and ≥ 1-log2-fold change) between the lycopene and control groups, where pathways associated with cellular function, metabolism, DNA repair, and anti-apoptosis were upregulated in the lycopene group. Lycopene supplementation to serum-free maturation medium neutralized excess ROS during maturation, enhanced blastocyst development and quality, and modulated the transcriptomic landscape.
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spelling pubmed-88683382022-02-25 Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile Sidi, Shehu Pascottini, Osvaldo Bogado Angel-Velez, Daniel Azari-Dolatabad, Nima Pavani, Krishna Chaitanya Residiwati, Gretania Meese, Tim Van Nieuwerburgh, Filip Kambai Bawa, Elias Alikidon Voh, Ambrose Olusegun Ayo, Joseph Van Soom, Ann Antioxidants (Basel) Article Bovine embryos are typically cultured at reduced oxygen tension to lower the impact of oxidative stress on embryo development. However, oocyte in vitro maturation (IVM) is performed at atmospheric oxygen tension since low oxygen during maturation has a negative impact on oocyte developmental competence. Lycopene, a carotenoid, acts as a powerful antioxidant and may protect the oocyte against oxidative stress during maturation at atmospheric oxygen conditions. Here, we assessed the effect of adding 0.2 μM lycopene (antioxidant), 5 μM menadione (pro-oxidant), and their combination on the generation of reactive oxygen species (ROS) in matured oocytes and the subsequent development, quality, and transcriptome of the blastocysts in a bovine in vitro model. ROS fluorescent intensity in matured oocytes was significantly lower in the lycopene group, and the resulting embryos showed a significantly higher blastocyst rate on day 8 and a lower apoptotic cell ratio than all other groups. Transcriptomic analysis disclosed a total of 296 differentially expressed genes (Benjamini–Hochberg-adjusted p < 0.05 and ≥ 1-log2-fold change) between the lycopene and control groups, where pathways associated with cellular function, metabolism, DNA repair, and anti-apoptosis were upregulated in the lycopene group. Lycopene supplementation to serum-free maturation medium neutralized excess ROS during maturation, enhanced blastocyst development and quality, and modulated the transcriptomic landscape. MDPI 2022-02-10 /pmc/articles/PMC8868338/ /pubmed/35204226 http://dx.doi.org/10.3390/antiox11020344 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sidi, Shehu
Pascottini, Osvaldo Bogado
Angel-Velez, Daniel
Azari-Dolatabad, Nima
Pavani, Krishna Chaitanya
Residiwati, Gretania
Meese, Tim
Van Nieuwerburgh, Filip
Kambai Bawa, Elias
Alikidon Voh, Ambrose
Olusegun Ayo, Joseph
Van Soom, Ann
Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile
title Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile
title_full Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile
title_fullStr Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile
title_full_unstemmed Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile
title_short Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile
title_sort lycopene supplementation to serum-free maturation medium improves in vitro bovine embryo development and quality and modulates embryonic transcriptomic profile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868338/
https://www.ncbi.nlm.nih.gov/pubmed/35204226
http://dx.doi.org/10.3390/antiox11020344
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