Effect of the In Ovo Injection Site of Electrolytes on Some Biochemical Blood Parameters and Quality of Layer Chicks

SIMPLE SUMMARY: In ovo technology is a unique method, the primary goal of which is to administer bioactive substances to eggs during the embryonic development of the bird. Therefore, it is necessary to develop optimal techniques for the implementation of in ovo feeding technology in practical poultry production. This preliminary study may open a window for future research on the site/location of manipulation and solutions and carriers of nutrients used for in ovo injection of laying hen embryos. ABSTRACT: The effects of the in ovo injection site of electrolytes on selected biochemical blood parameters and the quality of layer chicks were investigated. A total of 120 fertile eggs from Rhode Island Red breeders were randomly distributed into 4 groups, with each group including 30 birds. The groups were as follows: untreated control and groups with different injection sites/locations of 500 µL of 0.9% saline (NaCl) on day 18 of incubation, i.e., into the air cell (AC), through the air cell into the amniotic fluid (AFA), and directly into the amniotic fluid (AF). Measurement at 1 day of age showed that regardless of the injection site, embryos injected with 500 µL of saline had significantly higher Tona score (95/100 points) compared to the control group (90/100 points). Chick length was similar among the injected groups (mean 14.7 cm) and shorter in the control group (13.9 cm). There was no significant effect of in ovo injection on the biochemical blood parameters: total protein, cholesterol, high-density lipoprotein, low-density lipoprotein, glucose, urea, and uric acid. The highest concentration of sodium was noted in the control group (141.59 mmol/L). Regardless of the injection site/location, chicks treated with 500 µL of NaCl were characterized by a significantly lower blood sodium concentration (by 7.45% (AC), 7.90% (AFA), and 4.84% (AF) compared with birds from the control group (p ≤ 0.01)). The influence of saline solution administration in ovo on the blood potassium content of chicks was demonstrated. The concentration of potassium in the control group was significantly higher (by 11.36%) than in the AC group (p ≤ 0.01). In conclusion, the injection of 500 µL of saline solution into the developing chick embryo during the last days of incubation may have a positive effect on the quality of day-old chicks.