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Multi-Drug Resistant Escherichia coli, Biosecurity and Anti-Microbial Use in Live Bird Markets, Abeokuta, Nigeria

Live bird markets (LBM) remain a critical link from farm to fork in the poultry value chain, which oftentimes promotes indiscriminate antimicrobial use (AMU) and resistance (AMR). In this study, we assessed biosecurity practices, AMU, and associated these with multidrug resistant (MDR) E. coli in LB...

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Autores principales: Adebowale, Oluwawemimo, Makanjuola, Motunrayo, Bankole, Noah, Olasoju, Mary, Alamu, Aderonke, Kperegbeyi, Eniola, Oladejo, Oladotun, Fasanmi, Olubunmi, Adeyemo, Olanike, Fasina, Folorunso O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868421/
https://www.ncbi.nlm.nih.gov/pubmed/35203856
http://dx.doi.org/10.3390/antibiotics11020253
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author Adebowale, Oluwawemimo
Makanjuola, Motunrayo
Bankole, Noah
Olasoju, Mary
Alamu, Aderonke
Kperegbeyi, Eniola
Oladejo, Oladotun
Fasanmi, Olubunmi
Adeyemo, Olanike
Fasina, Folorunso O.
author_facet Adebowale, Oluwawemimo
Makanjuola, Motunrayo
Bankole, Noah
Olasoju, Mary
Alamu, Aderonke
Kperegbeyi, Eniola
Oladejo, Oladotun
Fasanmi, Olubunmi
Adeyemo, Olanike
Fasina, Folorunso O.
author_sort Adebowale, Oluwawemimo
collection PubMed
description Live bird markets (LBM) remain a critical link from farm to fork in the poultry value chain, which oftentimes promotes indiscriminate antimicrobial use (AMU) and resistance (AMR). In this study, we assessed biosecurity practices, AMU, and associated these with multidrug resistant (MDR) E. coli in LBMs in Abeokuta, Ogun State. A cross-sectional survey among live bird sellers (LBS) in eight LBMs was conducted using a semi-structured questionnaire. Also, cloacal samples (n = 200) were randomly collected and pooled for bacteriological detection of MDR E. coli in live chickens of consenting LBS. Susceptibility to 14 antimicrobials belonging to 6 different classes was determined using the disk diffusion method. Biosecurity level and AMU were generally low. LBS less than 46 years were 6.8- fold more likely to fall within the poor biosecurity level (Crudes odds ratio = 6.8; 95% CI; 1.20–38.56; p = 0.03) than others. An informal or primary school education increased the odds of having a poor practice of AMU by 15.1 folds (Crudes odds ratio = 15.1; 95% CI; 2.73–84.18; p = 0.002) than those with secondary or tertiary. The prevalence of E. coli and MDR E. coli at the LBM level were 80% and 56.3%, respectively. Extremely high resistance rates were observed for ceftazidime (96.9%) and imipenem (90.6%). The odds of MDR E. coli increased eight-fold in poultry kept by LBS who use AMs as prophylaxis. This current data could be useful for the development of targeted behavioral risk communication and mitigation strategies for AMR to impede the potential horizontal transfer of AMR genes to humans through animal-sourced food.
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spelling pubmed-88684212022-02-25 Multi-Drug Resistant Escherichia coli, Biosecurity and Anti-Microbial Use in Live Bird Markets, Abeokuta, Nigeria Adebowale, Oluwawemimo Makanjuola, Motunrayo Bankole, Noah Olasoju, Mary Alamu, Aderonke Kperegbeyi, Eniola Oladejo, Oladotun Fasanmi, Olubunmi Adeyemo, Olanike Fasina, Folorunso O. Antibiotics (Basel) Article Live bird markets (LBM) remain a critical link from farm to fork in the poultry value chain, which oftentimes promotes indiscriminate antimicrobial use (AMU) and resistance (AMR). In this study, we assessed biosecurity practices, AMU, and associated these with multidrug resistant (MDR) E. coli in LBMs in Abeokuta, Ogun State. A cross-sectional survey among live bird sellers (LBS) in eight LBMs was conducted using a semi-structured questionnaire. Also, cloacal samples (n = 200) were randomly collected and pooled for bacteriological detection of MDR E. coli in live chickens of consenting LBS. Susceptibility to 14 antimicrobials belonging to 6 different classes was determined using the disk diffusion method. Biosecurity level and AMU were generally low. LBS less than 46 years were 6.8- fold more likely to fall within the poor biosecurity level (Crudes odds ratio = 6.8; 95% CI; 1.20–38.56; p = 0.03) than others. An informal or primary school education increased the odds of having a poor practice of AMU by 15.1 folds (Crudes odds ratio = 15.1; 95% CI; 2.73–84.18; p = 0.002) than those with secondary or tertiary. The prevalence of E. coli and MDR E. coli at the LBM level were 80% and 56.3%, respectively. Extremely high resistance rates were observed for ceftazidime (96.9%) and imipenem (90.6%). The odds of MDR E. coli increased eight-fold in poultry kept by LBS who use AMs as prophylaxis. This current data could be useful for the development of targeted behavioral risk communication and mitigation strategies for AMR to impede the potential horizontal transfer of AMR genes to humans through animal-sourced food. MDPI 2022-02-16 /pmc/articles/PMC8868421/ /pubmed/35203856 http://dx.doi.org/10.3390/antibiotics11020253 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Adebowale, Oluwawemimo
Makanjuola, Motunrayo
Bankole, Noah
Olasoju, Mary
Alamu, Aderonke
Kperegbeyi, Eniola
Oladejo, Oladotun
Fasanmi, Olubunmi
Adeyemo, Olanike
Fasina, Folorunso O.
Multi-Drug Resistant Escherichia coli, Biosecurity and Anti-Microbial Use in Live Bird Markets, Abeokuta, Nigeria
title Multi-Drug Resistant Escherichia coli, Biosecurity and Anti-Microbial Use in Live Bird Markets, Abeokuta, Nigeria
title_full Multi-Drug Resistant Escherichia coli, Biosecurity and Anti-Microbial Use in Live Bird Markets, Abeokuta, Nigeria
title_fullStr Multi-Drug Resistant Escherichia coli, Biosecurity and Anti-Microbial Use in Live Bird Markets, Abeokuta, Nigeria
title_full_unstemmed Multi-Drug Resistant Escherichia coli, Biosecurity and Anti-Microbial Use in Live Bird Markets, Abeokuta, Nigeria
title_short Multi-Drug Resistant Escherichia coli, Biosecurity and Anti-Microbial Use in Live Bird Markets, Abeokuta, Nigeria
title_sort multi-drug resistant escherichia coli, biosecurity and anti-microbial use in live bird markets, abeokuta, nigeria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868421/
https://www.ncbi.nlm.nih.gov/pubmed/35203856
http://dx.doi.org/10.3390/antibiotics11020253
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