Using Pen-Side Measurable Blood Parameters to Predict or Identify Dystocic Lambing Events

SIMPLE SUMMARY: Prolonged or non-progressive labour is the greatest risk factor for loss of newborn lambs in Australia and poses significant welfare and economic concerns worldwide. In this study, we set out to investigate whether pen-side technology could be used to predict which ewes would be at risk of prolonged labour. In our pilot trial, we found potentially useful markers. We next developed a sampling protocol by looking at changes in candidate markers over time in normal lambing events. Finally, we searched for blood markers that could distinguish between normal and difficult lambing events, sampling pre-birth (estimated one week before birth), at birth (within 3 h) and post-birth (16–26 h). Possible predictors of lambing difficulty were chloride, haematocrit and haemoglobin, sampled one week before birth; creatinine, sampled at birth; and acid–base related parameters after birth. In conclusion, we found that pen-side analysis of blood markers showed promise in identifying dystocic lambing events. More information is required to decide whether pen-side diagnostics could be useful to identify and predict dystocic lambing in the future. ABSTRACT: Dystocia is the greatest contributor to neonatal lamb mortality in Australia and poses significant welfare and economic concerns worldwide. In this study, we set out to investigate whether pen-side analysis technology could be employed to detect blood parameters predictive of dystocic labour events in sheep. In a pilot trial, we collected and analysed blood samples in pen-side assays for glucose, lactate, pH, pCO(2), pO(2), base excess, HCO(3), TCO(2), sO(2), lactate, sodium, potassium, chloride, calcium, urea nitrogen, creatinine, haematocrit, haemoglobin and anion gap. From the pilot data, we identified creatinine, TCO(2), chloride and calcium as potentially useful markers. To develop a time course and to establish variability of the selected blood parameters, a time series of samples was collected from 12 ewes, from mid-gestation to 48 h after birth. For the main trial, blood samples were collected at mid- and late gestation for glucose determination and for the full set of blood parameters at three time points before, at and after birth. Possible predictors of lambing difficulty were chloride, haematocrit and haemoglobin, sampled one week before birth; creatinine, sampled at birth; and blood pH and base excess after birth. In conclusion, we found that pen-side analysis of blood markers showed promise in identifying dystocic lambing events.