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Microbiological Characteristics of Some Stations of Moscow Subway

SIMPLE SUMMARY: Public transport facilities, including subway systems, provide the most suitable conditions for the transfer of microorganisms between people and the environment, contributing to the pathogenic potential of the urban habitat. Investigation of microbiome diversity and description of i...

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Autores principales: Pochtovyi, Andrei A., Vasina, Daria V., Verdiev, Bakhtiyar I., Shchetinin, Alexey M., Yuzhakov, Anton G., Ovchinnikov, Roman S., Tkachuk, Artem P., Gushchin, Vladimir A., Gintsburg, Alexander L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869165/
https://www.ncbi.nlm.nih.gov/pubmed/35205037
http://dx.doi.org/10.3390/biology11020170
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author Pochtovyi, Andrei A.
Vasina, Daria V.
Verdiev, Bakhtiyar I.
Shchetinin, Alexey M.
Yuzhakov, Anton G.
Ovchinnikov, Roman S.
Tkachuk, Artem P.
Gushchin, Vladimir A.
Gintsburg, Alexander L.
author_facet Pochtovyi, Andrei A.
Vasina, Daria V.
Verdiev, Bakhtiyar I.
Shchetinin, Alexey M.
Yuzhakov, Anton G.
Ovchinnikov, Roman S.
Tkachuk, Artem P.
Gushchin, Vladimir A.
Gintsburg, Alexander L.
author_sort Pochtovyi, Andrei A.
collection PubMed
description SIMPLE SUMMARY: Public transport facilities, including subway systems, provide the most suitable conditions for the transfer of microorganisms between people and the environment, contributing to the pathogenic potential of the urban habitat. Investigation of microbiome diversity and description of its characteristic properties, e.g., antibiotic-resistance profiles, leads to understanding of these interactions. In this study, we aimed to conduct an extended analysis of the bioaerosol and surface microbiome of the Moscow subway, using 16S rRNA gene sample sequencing and classical microbiology methods. The microbiomes of two subway stations (Novokosino and Cherkizovskaya) were reconstructed which differ in terms of passenger traffic and duration of exploitation. It was shown that most bacterial genera were ubiquitous; however, the unique genera were presented in aerosol samples. The relatively older Cherkizovskaya station possessed greater diversity in antibiotic resistance among the identified microorganisms compared to Novokosino station. We also provided a comparative analysis of these results with the previously published data, which allowed us to identify the distribution of microorganisms associated with the human microbiome and the environment regardless of the seasonal fluctuations. The obtained results provide valuable information on the diversity of bacterial communities in the Moscow subway, one of the most socially important facilities in metropolitan areas. ABSTRACT: The subway is one of the most actively used means of transport in the traffic infrastructure of large metropolitan areas. More than seven million passengers use the Moscow subway every day, which promotes the exchange of microorganisms between people and the surrounding subway environment. In this research, a study of the bacterial communities of two Moscow subway stations was conducted and the common subway microbiome was determined. However, there were differences in microbiological and antibiotic-resistance profiles, depending on the station. The station’s operational period since opening correlated with the taxonomic diversity and resistance of the identified bacteria. Moreover, differences between aerosol and surface bacterial communities were found at the two subway stations, indicating the importance of diversified sampling during the microbiome profiling of public areas. In this study, we also compared our data with previously published results obtained for the Moscow subway. Despite sample collection at different stations and seasons, we showed the presence of 15 common genera forming the core microbiome of the Moscow subway, which represents human commensal species, as well as widespread microorganisms from the surrounding environment.
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spelling pubmed-88691652022-02-25 Microbiological Characteristics of Some Stations of Moscow Subway Pochtovyi, Andrei A. Vasina, Daria V. Verdiev, Bakhtiyar I. Shchetinin, Alexey M. Yuzhakov, Anton G. Ovchinnikov, Roman S. Tkachuk, Artem P. Gushchin, Vladimir A. Gintsburg, Alexander L. Biology (Basel) Article SIMPLE SUMMARY: Public transport facilities, including subway systems, provide the most suitable conditions for the transfer of microorganisms between people and the environment, contributing to the pathogenic potential of the urban habitat. Investigation of microbiome diversity and description of its characteristic properties, e.g., antibiotic-resistance profiles, leads to understanding of these interactions. In this study, we aimed to conduct an extended analysis of the bioaerosol and surface microbiome of the Moscow subway, using 16S rRNA gene sample sequencing and classical microbiology methods. The microbiomes of two subway stations (Novokosino and Cherkizovskaya) were reconstructed which differ in terms of passenger traffic and duration of exploitation. It was shown that most bacterial genera were ubiquitous; however, the unique genera were presented in aerosol samples. The relatively older Cherkizovskaya station possessed greater diversity in antibiotic resistance among the identified microorganisms compared to Novokosino station. We also provided a comparative analysis of these results with the previously published data, which allowed us to identify the distribution of microorganisms associated with the human microbiome and the environment regardless of the seasonal fluctuations. The obtained results provide valuable information on the diversity of bacterial communities in the Moscow subway, one of the most socially important facilities in metropolitan areas. ABSTRACT: The subway is one of the most actively used means of transport in the traffic infrastructure of large metropolitan areas. More than seven million passengers use the Moscow subway every day, which promotes the exchange of microorganisms between people and the surrounding subway environment. In this research, a study of the bacterial communities of two Moscow subway stations was conducted and the common subway microbiome was determined. However, there were differences in microbiological and antibiotic-resistance profiles, depending on the station. The station’s operational period since opening correlated with the taxonomic diversity and resistance of the identified bacteria. Moreover, differences between aerosol and surface bacterial communities were found at the two subway stations, indicating the importance of diversified sampling during the microbiome profiling of public areas. In this study, we also compared our data with previously published results obtained for the Moscow subway. Despite sample collection at different stations and seasons, we showed the presence of 15 common genera forming the core microbiome of the Moscow subway, which represents human commensal species, as well as widespread microorganisms from the surrounding environment. MDPI 2022-01-21 /pmc/articles/PMC8869165/ /pubmed/35205037 http://dx.doi.org/10.3390/biology11020170 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pochtovyi, Andrei A.
Vasina, Daria V.
Verdiev, Bakhtiyar I.
Shchetinin, Alexey M.
Yuzhakov, Anton G.
Ovchinnikov, Roman S.
Tkachuk, Artem P.
Gushchin, Vladimir A.
Gintsburg, Alexander L.
Microbiological Characteristics of Some Stations of Moscow Subway
title Microbiological Characteristics of Some Stations of Moscow Subway
title_full Microbiological Characteristics of Some Stations of Moscow Subway
title_fullStr Microbiological Characteristics of Some Stations of Moscow Subway
title_full_unstemmed Microbiological Characteristics of Some Stations of Moscow Subway
title_short Microbiological Characteristics of Some Stations of Moscow Subway
title_sort microbiological characteristics of some stations of moscow subway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869165/
https://www.ncbi.nlm.nih.gov/pubmed/35205037
http://dx.doi.org/10.3390/biology11020170
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