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A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869234/ https://www.ncbi.nlm.nih.gov/pubmed/35200320 http://dx.doi.org/10.3390/bios12020059 |
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author | Tang, Xiaoqian Zhang, Qi Isabel Pividori, Maria Zhang, Zhaowei Marty, Jean-Louis Catanante, Gaëlle |
author_facet | Tang, Xiaoqian Zhang, Qi Isabel Pividori, Maria Zhang, Zhaowei Marty, Jean-Louis Catanante, Gaëlle |
author_sort | Tang, Xiaoqian |
collection | PubMed |
description | Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B(1) (AFB(1)), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice. |
format | Online Article Text |
id | pubmed-8869234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88692342022-02-25 A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice Tang, Xiaoqian Zhang, Qi Isabel Pividori, Maria Zhang, Zhaowei Marty, Jean-Louis Catanante, Gaëlle Biosensors (Basel) Article Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B(1) (AFB(1)), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice. MDPI 2022-01-23 /pmc/articles/PMC8869234/ /pubmed/35200320 http://dx.doi.org/10.3390/bios12020059 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tang, Xiaoqian Zhang, Qi Isabel Pividori, Maria Zhang, Zhaowei Marty, Jean-Louis Catanante, Gaëlle A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice |
title | A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice |
title_full | A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice |
title_fullStr | A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice |
title_full_unstemmed | A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice |
title_short | A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice |
title_sort | sensitive aptasensor using biotin-streptavidin system for patulin detection in apple juice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869234/ https://www.ncbi.nlm.nih.gov/pubmed/35200320 http://dx.doi.org/10.3390/bios12020059 |
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