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Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix
Of all biologic matrices, decellularized tissues have emerged as a promising tool in the field of regenerative medicine. Few empirical clinical studies have shown that Wharton’s jelly (WJ) of the human umbilical cord promotes wound closure and reduces wound-related infections. In this scope, we here...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869352/ https://www.ncbi.nlm.nih.gov/pubmed/35203437 http://dx.doi.org/10.3390/biomedicines10020227 |
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author | Dubus, Marie Scomazzon, Loïc Chevrier, Julie Ledouble, Charlotte Baldit, Adrien Braux, Julien Gindraux, Florelle Boulagnon, Camille Audonnet, Sandra Colin, Marius Rammal, Hassan Mauprivez, Cédric Kerdjoudj, Halima |
author_facet | Dubus, Marie Scomazzon, Loïc Chevrier, Julie Ledouble, Charlotte Baldit, Adrien Braux, Julien Gindraux, Florelle Boulagnon, Camille Audonnet, Sandra Colin, Marius Rammal, Hassan Mauprivez, Cédric Kerdjoudj, Halima |
author_sort | Dubus, Marie |
collection | PubMed |
description | Of all biologic matrices, decellularized tissues have emerged as a promising tool in the field of regenerative medicine. Few empirical clinical studies have shown that Wharton’s jelly (WJ) of the human umbilical cord promotes wound closure and reduces wound-related infections. In this scope, we herein investigated whether decellularized (DC)-WJ could be used as an engineered biomaterial. In comparison with devitalized (DV)-WJ, our results showed an inherent effect of DC-WJ on Gram positive (S. aureus and S. epidermidis) and Gram negative (E. coli and P. aeruginosa) growth and adhesion. Although DC-WJ activated the neutrophils and monocytes in a comparable magnitude to DV-WJ, macrophages modulated their phenotypes and polarization states from the resting M0 phenotype to the hybrid M1/M2 phenotype in the presence of DC-WJ. M1 phenotype was predominant in the presence of DV-WJ. Finally, the subcutaneous implantation of DC-WJ showed total resorption after three weeks of implantation without any sign of foreign body reaction. These significant data shed light on the potential regenerative application of DC-WJ in providing a suitable biomaterial for tissue regenerative medicine and an ideal strategy to prevent wound-associated infections. |
format | Online Article Text |
id | pubmed-8869352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88693522022-02-25 Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix Dubus, Marie Scomazzon, Loïc Chevrier, Julie Ledouble, Charlotte Baldit, Adrien Braux, Julien Gindraux, Florelle Boulagnon, Camille Audonnet, Sandra Colin, Marius Rammal, Hassan Mauprivez, Cédric Kerdjoudj, Halima Biomedicines Article Of all biologic matrices, decellularized tissues have emerged as a promising tool in the field of regenerative medicine. Few empirical clinical studies have shown that Wharton’s jelly (WJ) of the human umbilical cord promotes wound closure and reduces wound-related infections. In this scope, we herein investigated whether decellularized (DC)-WJ could be used as an engineered biomaterial. In comparison with devitalized (DV)-WJ, our results showed an inherent effect of DC-WJ on Gram positive (S. aureus and S. epidermidis) and Gram negative (E. coli and P. aeruginosa) growth and adhesion. Although DC-WJ activated the neutrophils and monocytes in a comparable magnitude to DV-WJ, macrophages modulated their phenotypes and polarization states from the resting M0 phenotype to the hybrid M1/M2 phenotype in the presence of DC-WJ. M1 phenotype was predominant in the presence of DV-WJ. Finally, the subcutaneous implantation of DC-WJ showed total resorption after three weeks of implantation without any sign of foreign body reaction. These significant data shed light on the potential regenerative application of DC-WJ in providing a suitable biomaterial for tissue regenerative medicine and an ideal strategy to prevent wound-associated infections. MDPI 2022-01-21 /pmc/articles/PMC8869352/ /pubmed/35203437 http://dx.doi.org/10.3390/biomedicines10020227 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dubus, Marie Scomazzon, Loïc Chevrier, Julie Ledouble, Charlotte Baldit, Adrien Braux, Julien Gindraux, Florelle Boulagnon, Camille Audonnet, Sandra Colin, Marius Rammal, Hassan Mauprivez, Cédric Kerdjoudj, Halima Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix |
title | Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix |
title_full | Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix |
title_fullStr | Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix |
title_full_unstemmed | Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix |
title_short | Antibacterial and Immunomodulatory Properties of Acellular Wharton’s Jelly Matrix |
title_sort | antibacterial and immunomodulatory properties of acellular wharton’s jelly matrix |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869352/ https://www.ncbi.nlm.nih.gov/pubmed/35203437 http://dx.doi.org/10.3390/biomedicines10020227 |
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