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Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid

2,4-Dichlorophenoxyacetic acid (2,4-D) is a systemic conductive herbicide widely used across the world. With the large-scale and continuous use of 2,4-D, its possible harm to the environment and non-target organisms has attracted increasing attention, and the construction of a stable rapid on-site d...

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Autores principales: Zhou, Hui, He, Cong, Li, Zhenfeng, Huo, Jingqian, Xue, Yu, Xu, Xiaotong, Qi, Meng, Chen, Lai, Hammock, Bruce D., Zhang, Jinlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869386/
https://www.ncbi.nlm.nih.gov/pubmed/35200344
http://dx.doi.org/10.3390/bios12020084
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author Zhou, Hui
He, Cong
Li, Zhenfeng
Huo, Jingqian
Xue, Yu
Xu, Xiaotong
Qi, Meng
Chen, Lai
Hammock, Bruce D.
Zhang, Jinlin
author_facet Zhou, Hui
He, Cong
Li, Zhenfeng
Huo, Jingqian
Xue, Yu
Xu, Xiaotong
Qi, Meng
Chen, Lai
Hammock, Bruce D.
Zhang, Jinlin
author_sort Zhou, Hui
collection PubMed
description 2,4-Dichlorophenoxyacetic acid (2,4-D) is a systemic conductive herbicide widely used across the world. With the large-scale and continuous use of 2,4-D, its possible harm to the environment and non-target organisms has attracted increasing attention, and the construction of a stable rapid on-site detection method is particularly important. In order to achieve on-site rapid detection of 2,4-D, we developed a gold nanoparticle immunochromatographic strip method with the visual elimination value was 50 ng/mL, and a quantitative detection limit of 11 ng/mL based on a nanobody. By combing with the color snap, the immunochromatographic strip could quantitatively analyze the amounts of 2,4-D. Meanwhile, a colorimetric card based on the true color of the test strips was developed for the qualitative analysis of 2,4-D on-site. The samples (water, fruits and vegetables) with and without 2,4-D were detected by the immunochromatographic strips, and the results showed the accuracy and reliability. Thus, this assay is a rapid and simple on-site analytical tool to detect and quantify 2,4-D levels in environmental samples, and the analytical results can be obtained in about ten minutes. In addition, the nanobody technology used in this study provides an inexhaustible supply of a relatively stable antibodies that can be archived as a nanobody, plasmid or even its sequence.
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spelling pubmed-88693862022-02-25 Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid Zhou, Hui He, Cong Li, Zhenfeng Huo, Jingqian Xue, Yu Xu, Xiaotong Qi, Meng Chen, Lai Hammock, Bruce D. Zhang, Jinlin Biosensors (Basel) Article 2,4-Dichlorophenoxyacetic acid (2,4-D) is a systemic conductive herbicide widely used across the world. With the large-scale and continuous use of 2,4-D, its possible harm to the environment and non-target organisms has attracted increasing attention, and the construction of a stable rapid on-site detection method is particularly important. In order to achieve on-site rapid detection of 2,4-D, we developed a gold nanoparticle immunochromatographic strip method with the visual elimination value was 50 ng/mL, and a quantitative detection limit of 11 ng/mL based on a nanobody. By combing with the color snap, the immunochromatographic strip could quantitatively analyze the amounts of 2,4-D. Meanwhile, a colorimetric card based on the true color of the test strips was developed for the qualitative analysis of 2,4-D on-site. The samples (water, fruits and vegetables) with and without 2,4-D were detected by the immunochromatographic strips, and the results showed the accuracy and reliability. Thus, this assay is a rapid and simple on-site analytical tool to detect and quantify 2,4-D levels in environmental samples, and the analytical results can be obtained in about ten minutes. In addition, the nanobody technology used in this study provides an inexhaustible supply of a relatively stable antibodies that can be archived as a nanobody, plasmid or even its sequence. MDPI 2022-01-30 /pmc/articles/PMC8869386/ /pubmed/35200344 http://dx.doi.org/10.3390/bios12020084 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Hui
He, Cong
Li, Zhenfeng
Huo, Jingqian
Xue, Yu
Xu, Xiaotong
Qi, Meng
Chen, Lai
Hammock, Bruce D.
Zhang, Jinlin
Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid
title Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid
title_full Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid
title_fullStr Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid
title_full_unstemmed Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid
title_short Development of a Rapid Gold Nanoparticle Immunochromatographic Strip Based on the Nanobody for Detecting 2,4-DichloRophenoxyacetic Acid
title_sort development of a rapid gold nanoparticle immunochromatographic strip based on the nanobody for detecting 2,4-dichlorophenoxyacetic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869386/
https://www.ncbi.nlm.nih.gov/pubmed/35200344
http://dx.doi.org/10.3390/bios12020084
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