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Microfluidic Compartmentalization Platforms for Single Cell Analysis

Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technolo...

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Autores principales: Luo, Xuhao, Chen, Jui-Yi, Ataei, Marzieh, Lee, Abraham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869497/
https://www.ncbi.nlm.nih.gov/pubmed/35200319
http://dx.doi.org/10.3390/bios12020058
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author Luo, Xuhao
Chen, Jui-Yi
Ataei, Marzieh
Lee, Abraham
author_facet Luo, Xuhao
Chen, Jui-Yi
Ataei, Marzieh
Lee, Abraham
author_sort Luo, Xuhao
collection PubMed
description Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technology has revolutionized single-cell analysis through precise manipulation of liquid and compartmentalizing single cells in small volumes (pico- to nano-liter). Due to its advantages from miniaturization, microfluidic systems offer an array of capabilities to study genomics, transcriptomics, and proteomics of a large number of individual cells. In this regard, microfluidic systems have emerged as a powerful technology to uncover cellular heterogeneity and expand the depth and breadth of single-cell analysis. This review will focus on recent developments of three microfluidic compartmentalization platforms (microvalve, microwell, and microdroplets) that target single-cell analysis spanning from proteomics to genomics. We also compare and contrast these three microfluidic platforms and discuss their respective advantages and disadvantages in single-cell analysis.
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spelling pubmed-88694972022-02-25 Microfluidic Compartmentalization Platforms for Single Cell Analysis Luo, Xuhao Chen, Jui-Yi Ataei, Marzieh Lee, Abraham Biosensors (Basel) Review Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technology has revolutionized single-cell analysis through precise manipulation of liquid and compartmentalizing single cells in small volumes (pico- to nano-liter). Due to its advantages from miniaturization, microfluidic systems offer an array of capabilities to study genomics, transcriptomics, and proteomics of a large number of individual cells. In this regard, microfluidic systems have emerged as a powerful technology to uncover cellular heterogeneity and expand the depth and breadth of single-cell analysis. This review will focus on recent developments of three microfluidic compartmentalization platforms (microvalve, microwell, and microdroplets) that target single-cell analysis spanning from proteomics to genomics. We also compare and contrast these three microfluidic platforms and discuss their respective advantages and disadvantages in single-cell analysis. MDPI 2022-01-21 /pmc/articles/PMC8869497/ /pubmed/35200319 http://dx.doi.org/10.3390/bios12020058 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Luo, Xuhao
Chen, Jui-Yi
Ataei, Marzieh
Lee, Abraham
Microfluidic Compartmentalization Platforms for Single Cell Analysis
title Microfluidic Compartmentalization Platforms for Single Cell Analysis
title_full Microfluidic Compartmentalization Platforms for Single Cell Analysis
title_fullStr Microfluidic Compartmentalization Platforms for Single Cell Analysis
title_full_unstemmed Microfluidic Compartmentalization Platforms for Single Cell Analysis
title_short Microfluidic Compartmentalization Platforms for Single Cell Analysis
title_sort microfluidic compartmentalization platforms for single cell analysis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869497/
https://www.ncbi.nlm.nih.gov/pubmed/35200319
http://dx.doi.org/10.3390/bios12020058
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