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Microfluidic Compartmentalization Platforms for Single Cell Analysis
Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technolo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869497/ https://www.ncbi.nlm.nih.gov/pubmed/35200319 http://dx.doi.org/10.3390/bios12020058 |
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author | Luo, Xuhao Chen, Jui-Yi Ataei, Marzieh Lee, Abraham |
author_facet | Luo, Xuhao Chen, Jui-Yi Ataei, Marzieh Lee, Abraham |
author_sort | Luo, Xuhao |
collection | PubMed |
description | Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technology has revolutionized single-cell analysis through precise manipulation of liquid and compartmentalizing single cells in small volumes (pico- to nano-liter). Due to its advantages from miniaturization, microfluidic systems offer an array of capabilities to study genomics, transcriptomics, and proteomics of a large number of individual cells. In this regard, microfluidic systems have emerged as a powerful technology to uncover cellular heterogeneity and expand the depth and breadth of single-cell analysis. This review will focus on recent developments of three microfluidic compartmentalization platforms (microvalve, microwell, and microdroplets) that target single-cell analysis spanning from proteomics to genomics. We also compare and contrast these three microfluidic platforms and discuss their respective advantages and disadvantages in single-cell analysis. |
format | Online Article Text |
id | pubmed-8869497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88694972022-02-25 Microfluidic Compartmentalization Platforms for Single Cell Analysis Luo, Xuhao Chen, Jui-Yi Ataei, Marzieh Lee, Abraham Biosensors (Basel) Review Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technology has revolutionized single-cell analysis through precise manipulation of liquid and compartmentalizing single cells in small volumes (pico- to nano-liter). Due to its advantages from miniaturization, microfluidic systems offer an array of capabilities to study genomics, transcriptomics, and proteomics of a large number of individual cells. In this regard, microfluidic systems have emerged as a powerful technology to uncover cellular heterogeneity and expand the depth and breadth of single-cell analysis. This review will focus on recent developments of three microfluidic compartmentalization platforms (microvalve, microwell, and microdroplets) that target single-cell analysis spanning from proteomics to genomics. We also compare and contrast these three microfluidic platforms and discuss their respective advantages and disadvantages in single-cell analysis. MDPI 2022-01-21 /pmc/articles/PMC8869497/ /pubmed/35200319 http://dx.doi.org/10.3390/bios12020058 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Luo, Xuhao Chen, Jui-Yi Ataei, Marzieh Lee, Abraham Microfluidic Compartmentalization Platforms for Single Cell Analysis |
title | Microfluidic Compartmentalization Platforms for Single Cell Analysis |
title_full | Microfluidic Compartmentalization Platforms for Single Cell Analysis |
title_fullStr | Microfluidic Compartmentalization Platforms for Single Cell Analysis |
title_full_unstemmed | Microfluidic Compartmentalization Platforms for Single Cell Analysis |
title_short | Microfluidic Compartmentalization Platforms for Single Cell Analysis |
title_sort | microfluidic compartmentalization platforms for single cell analysis |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869497/ https://www.ncbi.nlm.nih.gov/pubmed/35200319 http://dx.doi.org/10.3390/bios12020058 |
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