First Report of Epimeletic and Acoustic Behavior in Mediterranean Common Bottlenose Dolphins (Tursiops truncatus) Carrying Dead Calves

SIMPLE SUMMARY: Death-related behaviors have been often observed in cetaceans, frequently with a female caregiver (presumed mother) supporting a dead calf by carrying, lifting, or sinking it, generally accompanied by other escorting individuals. However, how cetaceans experience death and if their behavior could be compared to human grieving is still largely unknown, as well as their acoustic production in such contexts. This report describes two cases of an adult common bottlenose dolphin (Tursiops truncatus) supporting a dead newborn and associated acoustic behavior in the Tiber River estuary area (Rome, Mediterranean Sea, Italy). In both cases, a main supporter (putative mother) was observed interacting with the carcass of a newborn by lifting (case 1) and sinking it (case 2), always escorted by another adult individual. Several vocalizations were recorded, including a signature whistle (a tonal sound with a repeated, stereotyped, individual-specific frequency modulation pattern) in the first case, likely emitted by the putative mother to seek aid from other individuals. This result appears reasonable since bottlenose dolphins are a highly vocal species, that communicates mainly through acoustic signals. These observations confirm the occurrence of death-related supportive behavior in bottlenose dolphins and report a preliminary description of associated vocalizations, providing additional information on this largely unknown topic. ABSTRACT: Epimeletic behavior toward dead calves has been frequently reported in cetaceans, mostly with females (presumed mothers) showing supportive behaviors such as carrying, lifting, or sinking, often assisted by “escort” individuals. However, information on acoustic production in such contexts is scarce. This report describes two observations of epimeletic behavior toward dead newborns in bottlenose dolphins and associated acoustic production. Data were collected at the Tiber River estuary (Rome, Mediterranean Sea, Italy) with one hydrophone for passive acoustic monitoring and two digital cameras. In both cases, an individual (presumed mother) acting as the main supporter and directly interacting with the carcass by lifting it (case 1) or sinking it (case 2) was observed. Another adult individual (escort) was present in both encounters showing standing-by and excitement behaviors (case 1) and supportive behavior (case 2). In both encounters, whistles, pulsed sounds, and bray-call elements were recorded. The consistent vocal activity observed likely conveyed context-specific information. A signature whistle in the first encounter was also recorded, likely emitted by the putative mother as a distress call. This report confirms the occurrence of epimeletic behavior in bottlenose dolphins and reports a preliminary description of the acoustic production when a dead calf is involved, providing additional information on this largely unknown topic.