Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

Macrophages (M(Φ)) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M(Φ) can exist in two extreme phenotypes: pro-inflammatory (M1) M(Φ) and anti-inflammatory (M2) M(Φ). These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFNγ and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M(Φ) have gained major attention, as they are functionally similar to human monocyte-derived M(Φ) and are receptive to genome editing. In this study, we polarised iPSC-derived M(Φ) to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised M(Φ).