A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples

In this study, forchlorfenuron (CPPU) was coupled with succinic anhydride to yield a CPPU hapten (CPPU-COOH), and a high-affinity monoclonal antibody (mAb) that can specifically recognize CPPU was produced. Using this mAb as a recognition reagent, a sensitive indirect competitive enzyme-linked immun...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xinmei, Xie, Bo, Cheng, Yongjian, Luo, Lin, Liang, Yifan, Xiao, Zhili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869720/
https://www.ncbi.nlm.nih.gov/pubmed/35200339
http://dx.doi.org/10.3390/bios12020078
_version_ 1784656563861979136
author Liu, Xinmei
Xie, Bo
Cheng, Yongjian
Luo, Lin
Liang, Yifan
Xiao, Zhili
author_facet Liu, Xinmei
Xie, Bo
Cheng, Yongjian
Luo, Lin
Liang, Yifan
Xiao, Zhili
author_sort Liu, Xinmei
collection PubMed
description In this study, forchlorfenuron (CPPU) was coupled with succinic anhydride to yield a CPPU hapten (CPPU-COOH), and a high-affinity monoclonal antibody (mAb) that can specifically recognize CPPU was produced. Using this mAb as a recognition reagent, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for CPPU was optimized, which exhibits an IC(50) of 1.04 ng/mL, a limit of detection of 0.16 ng/mL, and a linear range of 0.31–3.43 ng/mL for CPPU. Cross-reactivity percentages with six analogues were all below 6%. The average recovery rates for cucumber and orange samples were from 85.23% to 119.14%. The analysis results of this icELISA showed good consistency with those from liquid chromatography mass spectrometry. These results suggest that the proposed icELISA provides a sensitive, specific, and reliable strategy for CPPU detection in food samples.
format Online
Article
Text
id pubmed-8869720
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88697202022-02-25 A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples Liu, Xinmei Xie, Bo Cheng, Yongjian Luo, Lin Liang, Yifan Xiao, Zhili Biosensors (Basel) Article In this study, forchlorfenuron (CPPU) was coupled with succinic anhydride to yield a CPPU hapten (CPPU-COOH), and a high-affinity monoclonal antibody (mAb) that can specifically recognize CPPU was produced. Using this mAb as a recognition reagent, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for CPPU was optimized, which exhibits an IC(50) of 1.04 ng/mL, a limit of detection of 0.16 ng/mL, and a linear range of 0.31–3.43 ng/mL for CPPU. Cross-reactivity percentages with six analogues were all below 6%. The average recovery rates for cucumber and orange samples were from 85.23% to 119.14%. The analysis results of this icELISA showed good consistency with those from liquid chromatography mass spectrometry. These results suggest that the proposed icELISA provides a sensitive, specific, and reliable strategy for CPPU detection in food samples. MDPI 2022-01-28 /pmc/articles/PMC8869720/ /pubmed/35200339 http://dx.doi.org/10.3390/bios12020078 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xinmei
Xie, Bo
Cheng, Yongjian
Luo, Lin
Liang, Yifan
Xiao, Zhili
A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples
title A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples
title_full A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples
title_fullStr A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples
title_full_unstemmed A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples
title_short A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples
title_sort sensitive monoclonal-antibody-based elisa for forchlorfenuron residue analysis in food samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8869720/
https://www.ncbi.nlm.nih.gov/pubmed/35200339
http://dx.doi.org/10.3390/bios12020078
work_keys_str_mv AT liuxinmei asensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT xiebo asensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT chengyongjian asensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT luolin asensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT liangyifan asensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT xiaozhili asensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT liuxinmei sensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT xiebo sensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT chengyongjian sensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT luolin sensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT liangyifan sensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples
AT xiaozhili sensitivemonoclonalantibodybasedelisaforforchlorfenuronresidueanalysisinfoodsamples

Ejemplares similares