Cargando…

Colorimetric Biosensor Based on Magnetic Enzyme and Gold Nanorods for Visual Detection of Fish Freshness

Histamine, an important safety index for aquatic products, can also be used as a freshness indicator for red-fleshed fish. In this work, magnetic graphene oxide (Fe(3)O(4)@GO, MGO) was applied to immobilize diamine oxidase (DAO) through a method of adsorption and covalent bonding. Under the optimize...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Xia, Wu, Xiaotian, Zhuang, Shunqian, Zhang, Yucong, Ding, Yuting, Zhou, Xuxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870018/
https://www.ncbi.nlm.nih.gov/pubmed/35200395
http://dx.doi.org/10.3390/bios12020135
_version_ 1784656635672657920
author Xu, Xia
Wu, Xiaotian
Zhuang, Shunqian
Zhang, Yucong
Ding, Yuting
Zhou, Xuxia
author_facet Xu, Xia
Wu, Xiaotian
Zhuang, Shunqian
Zhang, Yucong
Ding, Yuting
Zhou, Xuxia
author_sort Xu, Xia
collection PubMed
description Histamine, an important safety index for aquatic products, can also be used as a freshness indicator for red-fleshed fish. In this work, magnetic graphene oxide (Fe(3)O(4)@GO, MGO) was applied to immobilize diamine oxidase (DAO) through a method of adsorption and covalent bonding. Under the optimized conditions, magnetic DAO prepared by adsorption immobilization had a higher enzyme activity than that of free enzyme, which was selected for the sensor construction. A colorimetric biosensor based on magnetic DAO induced etching of gold nanorods (AuNRs) was developed for the detection of histamine in fish. The developed biosensor showed an excellent response toward histamine with a low detection limit of 1.23 μM and had negligible interference from other diamines. With increasing the histamine concentration, the AuNRs after the reaction exhibited colors ranging from dark green to blue-green, blue, purple, red, and colorless. The etching induced multicolor change of AuNRs indicated the presence of different contents of histamine in mackerel during storage, and was consistent with the overall change in the content of the total volatile basic nitrogen (TVB-N). Thus, it was indicated that the proposed colorimetric biosensor with a naked-eye-detectable readout has a great potential to evaluate the freshness of red-fleshed fish high in histamine.
format Online
Article
Text
id pubmed-8870018
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88700182022-02-25 Colorimetric Biosensor Based on Magnetic Enzyme and Gold Nanorods for Visual Detection of Fish Freshness Xu, Xia Wu, Xiaotian Zhuang, Shunqian Zhang, Yucong Ding, Yuting Zhou, Xuxia Biosensors (Basel) Article Histamine, an important safety index for aquatic products, can also be used as a freshness indicator for red-fleshed fish. In this work, magnetic graphene oxide (Fe(3)O(4)@GO, MGO) was applied to immobilize diamine oxidase (DAO) through a method of adsorption and covalent bonding. Under the optimized conditions, magnetic DAO prepared by adsorption immobilization had a higher enzyme activity than that of free enzyme, which was selected for the sensor construction. A colorimetric biosensor based on magnetic DAO induced etching of gold nanorods (AuNRs) was developed for the detection of histamine in fish. The developed biosensor showed an excellent response toward histamine with a low detection limit of 1.23 μM and had negligible interference from other diamines. With increasing the histamine concentration, the AuNRs after the reaction exhibited colors ranging from dark green to blue-green, blue, purple, red, and colorless. The etching induced multicolor change of AuNRs indicated the presence of different contents of histamine in mackerel during storage, and was consistent with the overall change in the content of the total volatile basic nitrogen (TVB-N). Thus, it was indicated that the proposed colorimetric biosensor with a naked-eye-detectable readout has a great potential to evaluate the freshness of red-fleshed fish high in histamine. MDPI 2022-02-21 /pmc/articles/PMC8870018/ /pubmed/35200395 http://dx.doi.org/10.3390/bios12020135 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Xia
Wu, Xiaotian
Zhuang, Shunqian
Zhang, Yucong
Ding, Yuting
Zhou, Xuxia
Colorimetric Biosensor Based on Magnetic Enzyme and Gold Nanorods for Visual Detection of Fish Freshness
title Colorimetric Biosensor Based on Magnetic Enzyme and Gold Nanorods for Visual Detection of Fish Freshness
title_full Colorimetric Biosensor Based on Magnetic Enzyme and Gold Nanorods for Visual Detection of Fish Freshness
title_fullStr Colorimetric Biosensor Based on Magnetic Enzyme and Gold Nanorods for Visual Detection of Fish Freshness
title_full_unstemmed Colorimetric Biosensor Based on Magnetic Enzyme and Gold Nanorods for Visual Detection of Fish Freshness
title_short Colorimetric Biosensor Based on Magnetic Enzyme and Gold Nanorods for Visual Detection of Fish Freshness
title_sort colorimetric biosensor based on magnetic enzyme and gold nanorods for visual detection of fish freshness
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870018/
https://www.ncbi.nlm.nih.gov/pubmed/35200395
http://dx.doi.org/10.3390/bios12020135
work_keys_str_mv AT xuxia colorimetricbiosensorbasedonmagneticenzymeandgoldnanorodsforvisualdetectionoffishfreshness
AT wuxiaotian colorimetricbiosensorbasedonmagneticenzymeandgoldnanorodsforvisualdetectionoffishfreshness
AT zhuangshunqian colorimetricbiosensorbasedonmagneticenzymeandgoldnanorodsforvisualdetectionoffishfreshness
AT zhangyucong colorimetricbiosensorbasedonmagneticenzymeandgoldnanorodsforvisualdetectionoffishfreshness
AT dingyuting colorimetricbiosensorbasedonmagneticenzymeandgoldnanorodsforvisualdetectionoffishfreshness
AT zhouxuxia colorimetricbiosensorbasedonmagneticenzymeandgoldnanorodsforvisualdetectionoffishfreshness