Cargando…

Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review

SIMPLE SUMMARY: Cancer is one of the diseases with a high mortality rate worldwide. Of the current strategies to study new diagnostic and treating tools, organs-on-chip are quite promising regarding the achievement of more personalized medicine. In this work, 75 out of 820 of the most recent publish...

Descripción completa

Detalles Bibliográficos
Autores principales: Gonçalves, Inês M., Carvalho, Violeta, Rodrigues, Raquel O., Pinho, Diana, Teixeira, Senhorinha F. C. F., Moita, Ana, Hori, Takeshi, Kaji, Hirokazu, Lima, Rui, Minas, Graça
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870045/
https://www.ncbi.nlm.nih.gov/pubmed/35205683
http://dx.doi.org/10.3390/cancers14040935
_version_ 1784656642419195904
author Gonçalves, Inês M.
Carvalho, Violeta
Rodrigues, Raquel O.
Pinho, Diana
Teixeira, Senhorinha F. C. F.
Moita, Ana
Hori, Takeshi
Kaji, Hirokazu
Lima, Rui
Minas, Graça
author_facet Gonçalves, Inês M.
Carvalho, Violeta
Rodrigues, Raquel O.
Pinho, Diana
Teixeira, Senhorinha F. C. F.
Moita, Ana
Hori, Takeshi
Kaji, Hirokazu
Lima, Rui
Minas, Graça
author_sort Gonçalves, Inês M.
collection PubMed
description SIMPLE SUMMARY: Cancer is one of the diseases with a high mortality rate worldwide. Of the current strategies to study new diagnostic and treating tools, organs-on-chip are quite promising regarding the achievement of more personalized medicine. In this work, 75 out of 820 of the most recent published scientific articles were selected and analyzed through a systematic process. The selected articles present the different microfluidic platforms where cell culture was introduced and was used for the evaluation of cancer treatments efficacy and/or toxicity. ABSTRACT: The development of cancer models that rectify the simplicity of monolayer or static cell cultures physiologic microenvironment and, at the same time, replicate the human system more accurately than animal models has been a challenge in biomedical research. Organ-on-a-chip (OoC) devices are a solution that has been explored over the last decade. The combination of microfluidics and cell culture allows the design of a dynamic microenvironment suitable for the evaluation of treatments’ efficacy and effects, closer to the response observed in patients. This systematic review sums the studies from the last decade, where OoC with cancer cell cultures were used for drug screening assays. The studies were selected from three databases and analyzed following the research guidelines for systematic reviews proposed by PRISMA. In the selected studies, several types of cancer cells were evaluated, and the majority of treatments tested were standard chemotherapeutic drugs. Some studies reported higher drug resistance of the cultures on the OoC devices than on 2D cultures, which indicates the better resemblance to in vivo conditions of the former. Several studies also included the replication of the microvasculature or the combination of different cell cultures. The presence of vasculature can influence positively or negatively the drug efficacy since it contributes to a greater diffusion of the drug and also oxygen and nutrients. Co-cultures with liver cells contributed to the evaluation of the systemic toxicity of some drugs metabolites. Nevertheless, few studies used patient cells for the drug screening assays.
format Online
Article
Text
id pubmed-8870045
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88700452022-02-25 Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review Gonçalves, Inês M. Carvalho, Violeta Rodrigues, Raquel O. Pinho, Diana Teixeira, Senhorinha F. C. F. Moita, Ana Hori, Takeshi Kaji, Hirokazu Lima, Rui Minas, Graça Cancers (Basel) Review SIMPLE SUMMARY: Cancer is one of the diseases with a high mortality rate worldwide. Of the current strategies to study new diagnostic and treating tools, organs-on-chip are quite promising regarding the achievement of more personalized medicine. In this work, 75 out of 820 of the most recent published scientific articles were selected and analyzed through a systematic process. The selected articles present the different microfluidic platforms where cell culture was introduced and was used for the evaluation of cancer treatments efficacy and/or toxicity. ABSTRACT: The development of cancer models that rectify the simplicity of monolayer or static cell cultures physiologic microenvironment and, at the same time, replicate the human system more accurately than animal models has been a challenge in biomedical research. Organ-on-a-chip (OoC) devices are a solution that has been explored over the last decade. The combination of microfluidics and cell culture allows the design of a dynamic microenvironment suitable for the evaluation of treatments’ efficacy and effects, closer to the response observed in patients. This systematic review sums the studies from the last decade, where OoC with cancer cell cultures were used for drug screening assays. The studies were selected from three databases and analyzed following the research guidelines for systematic reviews proposed by PRISMA. In the selected studies, several types of cancer cells were evaluated, and the majority of treatments tested were standard chemotherapeutic drugs. Some studies reported higher drug resistance of the cultures on the OoC devices than on 2D cultures, which indicates the better resemblance to in vivo conditions of the former. Several studies also included the replication of the microvasculature or the combination of different cell cultures. The presence of vasculature can influence positively or negatively the drug efficacy since it contributes to a greater diffusion of the drug and also oxygen and nutrients. Co-cultures with liver cells contributed to the evaluation of the systemic toxicity of some drugs metabolites. Nevertheless, few studies used patient cells for the drug screening assays. MDPI 2022-02-13 /pmc/articles/PMC8870045/ /pubmed/35205683 http://dx.doi.org/10.3390/cancers14040935 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gonçalves, Inês M.
Carvalho, Violeta
Rodrigues, Raquel O.
Pinho, Diana
Teixeira, Senhorinha F. C. F.
Moita, Ana
Hori, Takeshi
Kaji, Hirokazu
Lima, Rui
Minas, Graça
Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review
title Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review
title_full Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review
title_fullStr Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review
title_full_unstemmed Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review
title_short Organ-on-a-Chip Platforms for Drug Screening and Delivery in Tumor Cells: A Systematic Review
title_sort organ-on-a-chip platforms for drug screening and delivery in tumor cells: a systematic review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870045/
https://www.ncbi.nlm.nih.gov/pubmed/35205683
http://dx.doi.org/10.3390/cancers14040935
work_keys_str_mv AT goncalvesinesm organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT carvalhovioleta organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT rodriguesraquelo organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT pinhodiana organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT teixeirasenhorinhafcf organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT moitaana organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT horitakeshi organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT kajihirokazu organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT limarui organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview
AT minasgraca organonachipplatformsfordrugscreeninganddeliveryintumorcellsasystematicreview