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Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology
Background: Intraocular lymphoma (IOL) presents a real challenge in daily diagnostics. Cyto- and/or histopathology of vitreous body represent the diagnostic cornerstones. Yet, false negative results remain common. Therefore, we analyzed the diagnostic significance of flow cytometry (FC) within the w...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870741/ https://www.ncbi.nlm.nih.gov/pubmed/35200564 http://dx.doi.org/10.3390/curroncol29020065 |
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author | Shumilov, Evgenii Mazzeo, Paolo Zinkernagel, Martin S. Legros, Myriam Porret, Naomi Romagna, Lorenz Haase, Detlef Lenz, Georg Novak, Urban Banz, Yara Pabst, Thomas Bacher, Ulrike |
author_facet | Shumilov, Evgenii Mazzeo, Paolo Zinkernagel, Martin S. Legros, Myriam Porret, Naomi Romagna, Lorenz Haase, Detlef Lenz, Georg Novak, Urban Banz, Yara Pabst, Thomas Bacher, Ulrike |
author_sort | Shumilov, Evgenii |
collection | PubMed |
description | Background: Intraocular lymphoma (IOL) presents a real challenge in daily diagnostics. Cyto- and/or histopathology of vitreous body represent the diagnostic cornerstones. Yet, false negative results remain common. Therefore, we analyzed the diagnostic significance of flow cytometry (FC) within the workup algorithm of IOL and compared its sensitivity with the results obtained from routine cytopathology and molecular genetics; Methods: Seven patients undergoing vitrectomy due to suspected IOL were investigated by FC and parallel cytopathology and, if available, digital droplet PCR (ddPCR) for MYD88 L265P; Results: Four out of seven patients were finally diagnosed with IOL. Among the IOL patients, cytopathology confirmed the presence of lymphoma cells in only two cases. In contrast, FC was positive for IOL in all four cases, and FC additionally confirmed the lack of IOL in the remaining patients. In IOL patients diagnosed by FC and with available ddPCR, the diagnosis of IOL was confirmed by the presence of the MYD88 L265P mutation in all three patients; Conclusions: The combination with FC was superior to cytopathology alone in the diagnostic work-up of IOL, and it showed an excellent correlation with ddPCR results. A comprehensive diagnostic panel consisting of cytopathology, FC and molecular genetics should be considered for the work-up of suspected IOL. |
format | Online Article Text |
id | pubmed-8870741 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88707412022-02-25 Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology Shumilov, Evgenii Mazzeo, Paolo Zinkernagel, Martin S. Legros, Myriam Porret, Naomi Romagna, Lorenz Haase, Detlef Lenz, Georg Novak, Urban Banz, Yara Pabst, Thomas Bacher, Ulrike Curr Oncol Brief Report Background: Intraocular lymphoma (IOL) presents a real challenge in daily diagnostics. Cyto- and/or histopathology of vitreous body represent the diagnostic cornerstones. Yet, false negative results remain common. Therefore, we analyzed the diagnostic significance of flow cytometry (FC) within the workup algorithm of IOL and compared its sensitivity with the results obtained from routine cytopathology and molecular genetics; Methods: Seven patients undergoing vitrectomy due to suspected IOL were investigated by FC and parallel cytopathology and, if available, digital droplet PCR (ddPCR) for MYD88 L265P; Results: Four out of seven patients were finally diagnosed with IOL. Among the IOL patients, cytopathology confirmed the presence of lymphoma cells in only two cases. In contrast, FC was positive for IOL in all four cases, and FC additionally confirmed the lack of IOL in the remaining patients. In IOL patients diagnosed by FC and with available ddPCR, the diagnosis of IOL was confirmed by the presence of the MYD88 L265P mutation in all three patients; Conclusions: The combination with FC was superior to cytopathology alone in the diagnostic work-up of IOL, and it showed an excellent correlation with ddPCR results. A comprehensive diagnostic panel consisting of cytopathology, FC and molecular genetics should be considered for the work-up of suspected IOL. MDPI 2022-01-31 /pmc/articles/PMC8870741/ /pubmed/35200564 http://dx.doi.org/10.3390/curroncol29020065 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Shumilov, Evgenii Mazzeo, Paolo Zinkernagel, Martin S. Legros, Myriam Porret, Naomi Romagna, Lorenz Haase, Detlef Lenz, Georg Novak, Urban Banz, Yara Pabst, Thomas Bacher, Ulrike Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology |
title | Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology |
title_full | Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology |
title_fullStr | Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology |
title_full_unstemmed | Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology |
title_short | Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology |
title_sort | comprehensive laboratory diagnostic workup for patients with suspected intraocular lymphoma including flow cytometry, molecular genetics and cytopathology |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870741/ https://www.ncbi.nlm.nih.gov/pubmed/35200564 http://dx.doi.org/10.3390/curroncol29020065 |
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