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Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading
The emergence of the Omicron SARS-CoV-2 variant caused public health concerns worldwide, raising the need for the improvement of rapid monitoring strategies. The present manuscript aimed at providing evidence of the utility of a diagnostic kit for the routine testing of SARS-CoV-2 infection as a cos...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870788/ https://www.ncbi.nlm.nih.gov/pubmed/35204558 http://dx.doi.org/10.3390/diagnostics12020467 |
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author | Caputo, Valerio Calvino, Giulia Strafella, Claudia Termine, Andrea Fabrizio, Carlo Trastulli, Giulia Ingrascì, Arcangela Peconi, Cristina Bardini, Silvia Rossini, Angelo Salvia, Antonino Borsellino, Giovanna Battistini, Luca Caltagirone, Carlo Cascella, Raffaella Giardina, Emiliano |
author_facet | Caputo, Valerio Calvino, Giulia Strafella, Claudia Termine, Andrea Fabrizio, Carlo Trastulli, Giulia Ingrascì, Arcangela Peconi, Cristina Bardini, Silvia Rossini, Angelo Salvia, Antonino Borsellino, Giovanna Battistini, Luca Caltagirone, Carlo Cascella, Raffaella Giardina, Emiliano |
author_sort | Caputo, Valerio |
collection | PubMed |
description | The emergence of the Omicron SARS-CoV-2 variant caused public health concerns worldwide, raising the need for the improvement of rapid monitoring strategies. The present manuscript aimed at providing evidence of the utility of a diagnostic kit for the routine testing of SARS-CoV-2 infection as a cost-effective method for tracking the Omicron variant in Italy. The study was conducted on patients’ naso-oropharyngeal-swab-derived RNA samples. These samples were subjected to RT-PCR using the TaqPath COVID-19 RT PCR CE IVD kit. Nonparametric testing and polynomial models fitting were used to compare the spreading of Alpha, Delta and Omicron variants. The samples of interest were correctly amplified and displayed the presence of S gene-target failure, suggesting that these patients carry the Omicron variant. The trend of diffusion was found to be significantly different and more rapid compared with that of the Alpha and Delta variants in our cohorts. Overall, these results highlight that the S gene target failure was a very useful tool for the immediate and inexpensive tracking of Omicron variant in the three weeks from the first detection. Thus, our approach could be used as a first-line screening to reduce the time and costs of monitoring strategies, facilitating the management of preventive and counteracting measures against COVID-19. |
format | Online Article Text |
id | pubmed-8870788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88707882022-02-25 Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading Caputo, Valerio Calvino, Giulia Strafella, Claudia Termine, Andrea Fabrizio, Carlo Trastulli, Giulia Ingrascì, Arcangela Peconi, Cristina Bardini, Silvia Rossini, Angelo Salvia, Antonino Borsellino, Giovanna Battistini, Luca Caltagirone, Carlo Cascella, Raffaella Giardina, Emiliano Diagnostics (Basel) Communication The emergence of the Omicron SARS-CoV-2 variant caused public health concerns worldwide, raising the need for the improvement of rapid monitoring strategies. The present manuscript aimed at providing evidence of the utility of a diagnostic kit for the routine testing of SARS-CoV-2 infection as a cost-effective method for tracking the Omicron variant in Italy. The study was conducted on patients’ naso-oropharyngeal-swab-derived RNA samples. These samples were subjected to RT-PCR using the TaqPath COVID-19 RT PCR CE IVD kit. Nonparametric testing and polynomial models fitting were used to compare the spreading of Alpha, Delta and Omicron variants. The samples of interest were correctly amplified and displayed the presence of S gene-target failure, suggesting that these patients carry the Omicron variant. The trend of diffusion was found to be significantly different and more rapid compared with that of the Alpha and Delta variants in our cohorts. Overall, these results highlight that the S gene target failure was a very useful tool for the immediate and inexpensive tracking of Omicron variant in the three weeks from the first detection. Thus, our approach could be used as a first-line screening to reduce the time and costs of monitoring strategies, facilitating the management of preventive and counteracting measures against COVID-19. MDPI 2022-02-11 /pmc/articles/PMC8870788/ /pubmed/35204558 http://dx.doi.org/10.3390/diagnostics12020467 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Caputo, Valerio Calvino, Giulia Strafella, Claudia Termine, Andrea Fabrizio, Carlo Trastulli, Giulia Ingrascì, Arcangela Peconi, Cristina Bardini, Silvia Rossini, Angelo Salvia, Antonino Borsellino, Giovanna Battistini, Luca Caltagirone, Carlo Cascella, Raffaella Giardina, Emiliano Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading |
title | Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading |
title_full | Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading |
title_fullStr | Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading |
title_full_unstemmed | Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading |
title_short | Tracking the Initial Diffusion of SARS-CoV-2 Omicron Variant in Italy by RT-PCR and Comparison with Alpha and Delta Variants Spreading |
title_sort | tracking the initial diffusion of sars-cov-2 omicron variant in italy by rt-pcr and comparison with alpha and delta variants spreading |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870788/ https://www.ncbi.nlm.nih.gov/pubmed/35204558 http://dx.doi.org/10.3390/diagnostics12020467 |
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