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Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis

Patients with rheumatoid arthritis (RA) show autoantibodies against post-translational protein modifications (PTMs), such as anti-citrullinated protein antibodies. However, the range of recognized PTMs is unknown. Here, we addressed four PTMs: chlorination, non-enzymatic glycation, nitration, and ho...

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Autores principales: Rodríguez-Martínez, Lorena, Regueiro, Cristina, Amhaz-Escanlar, Sámer, Pena, Carmen, Herbello-Hermelo, Paloma, Moreda-Piñeiro, Antonio, Rodriguez-Garcia, Javier, Mera-Varela, Antonio, Pérez-Pampín, Eva, González, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870974/
https://www.ncbi.nlm.nih.gov/pubmed/35204444
http://dx.doi.org/10.3390/diagnostics12020352
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author Rodríguez-Martínez, Lorena
Regueiro, Cristina
Amhaz-Escanlar, Sámer
Pena, Carmen
Herbello-Hermelo, Paloma
Moreda-Piñeiro, Antonio
Rodriguez-Garcia, Javier
Mera-Varela, Antonio
Pérez-Pampín, Eva
González, Antonio
author_facet Rodríguez-Martínez, Lorena
Regueiro, Cristina
Amhaz-Escanlar, Sámer
Pena, Carmen
Herbello-Hermelo, Paloma
Moreda-Piñeiro, Antonio
Rodriguez-Garcia, Javier
Mera-Varela, Antonio
Pérez-Pampín, Eva
González, Antonio
author_sort Rodríguez-Martínez, Lorena
collection PubMed
description Patients with rheumatoid arthritis (RA) show autoantibodies against post-translational protein modifications (PTMs), such as anti-citrullinated protein antibodies. However, the range of recognized PTMs is unknown. Here, we addressed four PTMs: chlorination, non-enzymatic glycation, nitration, and homocysteinylation, identified as targets of atypical RA autoantibodies in studies whose protocols we have followed. The modified antigens included collagen type II, an extract of synovial proteins and a selection of peptides. We interpreted the results according to the optical density (OD) obtained in an enzyme-linked immunosorbent assay ( ELISA) with the modified antigen and the corrected OD obtained after subtracting the reactivity against the unmodified antigen. The results showed evidence of specific antibodies against glycated collagen type II, as the corrected ODs were higher in the 182 patients with RA than in the 164 healthy controls (p = 0.0003). However, the relevance of these antibodies was doubtful because the magnitude of the specific signal was small (median OD = 0.072 vs. 0.027, respectively). There were no specific antibodies against any of the other three PTMs. Therefore, our results showed that the four PTMs are not inducing a significant autoantibody response in patients with RA. These results indicated that the repertoire of PTM autoantigens in RA is restricted.
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spelling pubmed-88709742022-02-25 Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis Rodríguez-Martínez, Lorena Regueiro, Cristina Amhaz-Escanlar, Sámer Pena, Carmen Herbello-Hermelo, Paloma Moreda-Piñeiro, Antonio Rodriguez-Garcia, Javier Mera-Varela, Antonio Pérez-Pampín, Eva González, Antonio Diagnostics (Basel) Article Patients with rheumatoid arthritis (RA) show autoantibodies against post-translational protein modifications (PTMs), such as anti-citrullinated protein antibodies. However, the range of recognized PTMs is unknown. Here, we addressed four PTMs: chlorination, non-enzymatic glycation, nitration, and homocysteinylation, identified as targets of atypical RA autoantibodies in studies whose protocols we have followed. The modified antigens included collagen type II, an extract of synovial proteins and a selection of peptides. We interpreted the results according to the optical density (OD) obtained in an enzyme-linked immunosorbent assay ( ELISA) with the modified antigen and the corrected OD obtained after subtracting the reactivity against the unmodified antigen. The results showed evidence of specific antibodies against glycated collagen type II, as the corrected ODs were higher in the 182 patients with RA than in the 164 healthy controls (p = 0.0003). However, the relevance of these antibodies was doubtful because the magnitude of the specific signal was small (median OD = 0.072 vs. 0.027, respectively). There were no specific antibodies against any of the other three PTMs. Therefore, our results showed that the four PTMs are not inducing a significant autoantibody response in patients with RA. These results indicated that the repertoire of PTM autoantigens in RA is restricted. MDPI 2022-01-29 /pmc/articles/PMC8870974/ /pubmed/35204444 http://dx.doi.org/10.3390/diagnostics12020352 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez-Martínez, Lorena
Regueiro, Cristina
Amhaz-Escanlar, Sámer
Pena, Carmen
Herbello-Hermelo, Paloma
Moreda-Piñeiro, Antonio
Rodriguez-Garcia, Javier
Mera-Varela, Antonio
Pérez-Pampín, Eva
González, Antonio
Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis
title Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis
title_full Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis
title_fullStr Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis
title_full_unstemmed Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis
title_short Antibodies against 4 Atypical Post-Translational Protein Modifications in Patients with Rheumatoid Arthritis
title_sort antibodies against 4 atypical post-translational protein modifications in patients with rheumatoid arthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8870974/
https://www.ncbi.nlm.nih.gov/pubmed/35204444
http://dx.doi.org/10.3390/diagnostics12020352
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