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Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis

Early diagnosis increases the treatment success rate for active tuberculosis (ATB) and decreases mortality. MicroRNAs (miRNAs) have been studied as blood-based markers of several infectious diseases. We performed miRNA profiling to identify differentially expressed (DE) miRNAs using whole blood samp...

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Autores principales: Kim, Junseong, Park, Heechul, Park, Sung-Bae, Lee, Eun Ju, Je, Min-A, Ahn, Eunsol, Sim, Bora, Lee, Jiyoung, Jin, Hyunwoo, Lee, Kyung Eun, Cho, Sang-Nae, Kang, Young Ae, Lee, Hyejon, Kim, Sunghyun, Kim, Jungho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871062/
https://www.ncbi.nlm.nih.gov/pubmed/35204460
http://dx.doi.org/10.3390/diagnostics12020369
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author Kim, Junseong
Park, Heechul
Park, Sung-Bae
Lee, Eun Ju
Je, Min-A
Ahn, Eunsol
Sim, Bora
Lee, Jiyoung
Jin, Hyunwoo
Lee, Kyung Eun
Cho, Sang-Nae
Kang, Young Ae
Lee, Hyejon
Kim, Sunghyun
Kim, Jungho
author_facet Kim, Junseong
Park, Heechul
Park, Sung-Bae
Lee, Eun Ju
Je, Min-A
Ahn, Eunsol
Sim, Bora
Lee, Jiyoung
Jin, Hyunwoo
Lee, Kyung Eun
Cho, Sang-Nae
Kang, Young Ae
Lee, Hyejon
Kim, Sunghyun
Kim, Jungho
author_sort Kim, Junseong
collection PubMed
description Early diagnosis increases the treatment success rate for active tuberculosis (ATB) and decreases mortality. MicroRNAs (miRNAs) have been studied as blood-based markers of several infectious diseases. We performed miRNA profiling to identify differentially expressed (DE) miRNAs using whole blood samples from 10 healthy controls (HCs), 15 subjects with latent tuberculosis infection (LTBI), and 12 patients with ATB, and investigated the expression of the top six miRNAs at diagnosis and over the treatment period in addition to performing miRNA-target gene network and gene ontology analyses. miRNA profiling identified 84 DE miRNAs in patients with ATB, including 80 upregulated and four downregulated miRNAs. Receiver operating characteristic curves of the top six miRNAs exhibited excellent distinguishing efficiency with an area under curve (AUC) value > 0.85. Among them, miR-199a-3p and miR-6886-3p can differentiate between ATB and LTBI. Anti-TB treatment restored the levels of miR-199b-3p, miR-199a-3p, miR-16-5p, and miR-374c-5p to HC levels. Furthermore, 108 predicted target genes were related to the regulation of cellular amide metabolism, intrinsic apoptotic signaling, translation, transforming growth factor beta receptor signaling, and cysteine-type endopeptidase activity. The DE miRNAs identified herein are potential biomarkers for diagnosis and therapeutic monitoring in ATB.
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spelling pubmed-88710622022-02-25 Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis Kim, Junseong Park, Heechul Park, Sung-Bae Lee, Eun Ju Je, Min-A Ahn, Eunsol Sim, Bora Lee, Jiyoung Jin, Hyunwoo Lee, Kyung Eun Cho, Sang-Nae Kang, Young Ae Lee, Hyejon Kim, Sunghyun Kim, Jungho Diagnostics (Basel) Article Early diagnosis increases the treatment success rate for active tuberculosis (ATB) and decreases mortality. MicroRNAs (miRNAs) have been studied as blood-based markers of several infectious diseases. We performed miRNA profiling to identify differentially expressed (DE) miRNAs using whole blood samples from 10 healthy controls (HCs), 15 subjects with latent tuberculosis infection (LTBI), and 12 patients with ATB, and investigated the expression of the top six miRNAs at diagnosis and over the treatment period in addition to performing miRNA-target gene network and gene ontology analyses. miRNA profiling identified 84 DE miRNAs in patients with ATB, including 80 upregulated and four downregulated miRNAs. Receiver operating characteristic curves of the top six miRNAs exhibited excellent distinguishing efficiency with an area under curve (AUC) value > 0.85. Among them, miR-199a-3p and miR-6886-3p can differentiate between ATB and LTBI. Anti-TB treatment restored the levels of miR-199b-3p, miR-199a-3p, miR-16-5p, and miR-374c-5p to HC levels. Furthermore, 108 predicted target genes were related to the regulation of cellular amide metabolism, intrinsic apoptotic signaling, translation, transforming growth factor beta receptor signaling, and cysteine-type endopeptidase activity. The DE miRNAs identified herein are potential biomarkers for diagnosis and therapeutic monitoring in ATB. MDPI 2022-02-01 /pmc/articles/PMC8871062/ /pubmed/35204460 http://dx.doi.org/10.3390/diagnostics12020369 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Junseong
Park, Heechul
Park, Sung-Bae
Lee, Eun Ju
Je, Min-A
Ahn, Eunsol
Sim, Bora
Lee, Jiyoung
Jin, Hyunwoo
Lee, Kyung Eun
Cho, Sang-Nae
Kang, Young Ae
Lee, Hyejon
Kim, Sunghyun
Kim, Jungho
Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis
title Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis
title_full Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis
title_fullStr Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis
title_full_unstemmed Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis
title_short Identification of MicroRNAs as Potential Blood-Based Biomarkers for Diagnosis and Therapeutic Monitoring of Active Tuberculosis
title_sort identification of micrornas as potential blood-based biomarkers for diagnosis and therapeutic monitoring of active tuberculosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871062/
https://www.ncbi.nlm.nih.gov/pubmed/35204460
http://dx.doi.org/10.3390/diagnostics12020369
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