Cargando…
Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice
The potential of soft-shelled turtle peptides (STP) against fatigue was evaluated. Mice orally supplemented with STP significantly increased the swimming time until tiredness by 35.4–57.1%. Although not statistically significant, STP increased muscle and thymus mass. In addition, the serum lactate,...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871340/ https://www.ncbi.nlm.nih.gov/pubmed/35206076 http://dx.doi.org/10.3390/foods11040600 |
_version_ | 1784656973379141632 |
---|---|
author | Zhong, Hao Shi, Jinyuan Zhang, Junhui Wang, Qianqian Zhang, Yipeng Yu, Peng Guan, Rongfa Feng, Fengqin |
author_facet | Zhong, Hao Shi, Jinyuan Zhang, Junhui Wang, Qianqian Zhang, Yipeng Yu, Peng Guan, Rongfa Feng, Fengqin |
author_sort | Zhong, Hao |
collection | PubMed |
description | The potential of soft-shelled turtle peptides (STP) against fatigue was evaluated. Mice orally supplemented with STP significantly increased the swimming time until tiredness by 35.4–57.1%. Although not statistically significant, STP increased muscle and thymus mass. In addition, the serum lactate, ammonia, blood urea nitrogen content and creatine kinase activity in STP-fed mice were dramatically decreased when compared to the control group. Furthermore, STP supplementation increased the reserves of liver glycogen and muscle glycogen, thus improved the energy metabolism system of mice. STP treatment contributed to increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities as well as a decrease in malondialdehyde (MDA), indicating an improvement in oxidative stress protection. The Western blot (WB) results indicated that the STP supplement effectively altered the expression of oxidative stress-related protein by modulating the NRF2/KEAP1 pathway. In summary, STP affected NRF2/KEAP1 levels in skeletal muscle, leading to antioxidant activity and a slower time to exhaustion during exercise. |
format | Online Article Text |
id | pubmed-8871340 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88713402022-02-25 Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice Zhong, Hao Shi, Jinyuan Zhang, Junhui Wang, Qianqian Zhang, Yipeng Yu, Peng Guan, Rongfa Feng, Fengqin Foods Article The potential of soft-shelled turtle peptides (STP) against fatigue was evaluated. Mice orally supplemented with STP significantly increased the swimming time until tiredness by 35.4–57.1%. Although not statistically significant, STP increased muscle and thymus mass. In addition, the serum lactate, ammonia, blood urea nitrogen content and creatine kinase activity in STP-fed mice were dramatically decreased when compared to the control group. Furthermore, STP supplementation increased the reserves of liver glycogen and muscle glycogen, thus improved the energy metabolism system of mice. STP treatment contributed to increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities as well as a decrease in malondialdehyde (MDA), indicating an improvement in oxidative stress protection. The Western blot (WB) results indicated that the STP supplement effectively altered the expression of oxidative stress-related protein by modulating the NRF2/KEAP1 pathway. In summary, STP affected NRF2/KEAP1 levels in skeletal muscle, leading to antioxidant activity and a slower time to exhaustion during exercise. MDPI 2022-02-19 /pmc/articles/PMC8871340/ /pubmed/35206076 http://dx.doi.org/10.3390/foods11040600 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhong, Hao Shi, Jinyuan Zhang, Junhui Wang, Qianqian Zhang, Yipeng Yu, Peng Guan, Rongfa Feng, Fengqin Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice |
title | Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice |
title_full | Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice |
title_fullStr | Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice |
title_full_unstemmed | Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice |
title_short | Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice |
title_sort | soft-shelled turtle peptide supplementation modifies energy metabolism and oxidative stress, enhances exercise endurance, and decreases physical fatigue in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871340/ https://www.ncbi.nlm.nih.gov/pubmed/35206076 http://dx.doi.org/10.3390/foods11040600 |
work_keys_str_mv | AT zhonghao softshelledturtlepeptidesupplementationmodifiesenergymetabolismandoxidativestressenhancesexerciseenduranceanddecreasesphysicalfatigueinmice AT shijinyuan softshelledturtlepeptidesupplementationmodifiesenergymetabolismandoxidativestressenhancesexerciseenduranceanddecreasesphysicalfatigueinmice AT zhangjunhui softshelledturtlepeptidesupplementationmodifiesenergymetabolismandoxidativestressenhancesexerciseenduranceanddecreasesphysicalfatigueinmice AT wangqianqian softshelledturtlepeptidesupplementationmodifiesenergymetabolismandoxidativestressenhancesexerciseenduranceanddecreasesphysicalfatigueinmice AT zhangyipeng softshelledturtlepeptidesupplementationmodifiesenergymetabolismandoxidativestressenhancesexerciseenduranceanddecreasesphysicalfatigueinmice AT yupeng softshelledturtlepeptidesupplementationmodifiesenergymetabolismandoxidativestressenhancesexerciseenduranceanddecreasesphysicalfatigueinmice AT guanrongfa softshelledturtlepeptidesupplementationmodifiesenergymetabolismandoxidativestressenhancesexerciseenduranceanddecreasesphysicalfatigueinmice AT fengfengqin softshelledturtlepeptidesupplementationmodifiesenergymetabolismandoxidativestressenhancesexerciseenduranceanddecreasesphysicalfatigueinmice |