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Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens

Nanos is a translational regulator that is involved in germline development in a number of diverse animals and is also involved in somatic patterning in several model organisms, including insects. Neither germline development nor somatic stem cell lines/undifferentiated multipotent cells have been c...

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Autor principal: Kostyuchenko, Roman P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871563/
https://www.ncbi.nlm.nih.gov/pubmed/35205316
http://dx.doi.org/10.3390/genes13020270
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author Kostyuchenko, Roman P.
author_facet Kostyuchenko, Roman P.
author_sort Kostyuchenko, Roman P.
collection PubMed
description Nanos is a translational regulator that is involved in germline development in a number of diverse animals and is also involved in somatic patterning in several model organisms, including insects. Neither germline development nor somatic stem cell lines/undifferentiated multipotent cells have been characterized in the development of the annelid Alitta virens, nor is the mechanism of germ/stem-line specification generally well-understood in annelids. Here, I have cloned an Avi-nanos ortholog from A. virens and determined the spatial and temporal expression of Nanos. The results revealed that transcripts of nanos are expressed during differentiation of multiple tissues, including those that are derived from the 2d and 4d cells. In late embryonic stages and during larval development, these transcripts are expressed in the presumptive brain, ventral nerve cord, mesodermal bands, putative primordial germ cells (PGCs), and developing foregut and hindgut. During metamorphosis of the nectochaete larva into a juvenile worm, a posterior growth zone consisting of nanos-positive cells is established, and the PGCs begin to migrate. Later, the PGCs stop migrating and form a cluster of four nanos-expressing cells located immediately behind the jaws (segments 4–5). During posterior regeneration following caudal amputation, a robust Avi-nanos expression appears de novo at the site of injury and further accompanies all steps of regeneration. The obtained data suggest that blastemal cells are mostly derived from cells of the segment adjacent to the amputation site; this is consistent with the idea that the cluster of PGCs do not participate in regeneration.
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spelling pubmed-88715632022-02-25 Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens Kostyuchenko, Roman P. Genes (Basel) Article Nanos is a translational regulator that is involved in germline development in a number of diverse animals and is also involved in somatic patterning in several model organisms, including insects. Neither germline development nor somatic stem cell lines/undifferentiated multipotent cells have been characterized in the development of the annelid Alitta virens, nor is the mechanism of germ/stem-line specification generally well-understood in annelids. Here, I have cloned an Avi-nanos ortholog from A. virens and determined the spatial and temporal expression of Nanos. The results revealed that transcripts of nanos are expressed during differentiation of multiple tissues, including those that are derived from the 2d and 4d cells. In late embryonic stages and during larval development, these transcripts are expressed in the presumptive brain, ventral nerve cord, mesodermal bands, putative primordial germ cells (PGCs), and developing foregut and hindgut. During metamorphosis of the nectochaete larva into a juvenile worm, a posterior growth zone consisting of nanos-positive cells is established, and the PGCs begin to migrate. Later, the PGCs stop migrating and form a cluster of four nanos-expressing cells located immediately behind the jaws (segments 4–5). During posterior regeneration following caudal amputation, a robust Avi-nanos expression appears de novo at the site of injury and further accompanies all steps of regeneration. The obtained data suggest that blastemal cells are mostly derived from cells of the segment adjacent to the amputation site; this is consistent with the idea that the cluster of PGCs do not participate in regeneration. MDPI 2022-01-29 /pmc/articles/PMC8871563/ /pubmed/35205316 http://dx.doi.org/10.3390/genes13020270 Text en © 2022 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kostyuchenko, Roman P.
Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens
title Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens
title_full Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens
title_fullStr Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens
title_full_unstemmed Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens
title_short Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens
title_sort nanos is expressed in somatic and germline tissue during larval and post-larval development of the annelid alitta virens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871563/
https://www.ncbi.nlm.nih.gov/pubmed/35205316
http://dx.doi.org/10.3390/genes13020270
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