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Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model

CONTEXT: Cryptotanshinone (CT), a lipophilic compound extracted from roots of Salvia miltiorrhiza Bunge (Lamiaceae) (Danshen), has multiple properties in diseases, such as pulmonary fibrosis, lung cancer, and osteoarthritis. Our previous findings suggest that CT plays a protective role in cerebral s...

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Autores principales: Zhu, Feihong, Chen, Hehe, Xu, Meifei, Zhang, Xiajun, Yu, Jing, Pan, Yali, Zhu, Weixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871624/
https://www.ncbi.nlm.nih.gov/pubmed/33915069
http://dx.doi.org/10.1080/13880209.2021.1914672
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author Zhu, Feihong
Chen, Hehe
Xu, Meifei
Zhang, Xiajun
Yu, Jing
Pan, Yali
Zhu, Weixin
author_facet Zhu, Feihong
Chen, Hehe
Xu, Meifei
Zhang, Xiajun
Yu, Jing
Pan, Yali
Zhu, Weixin
author_sort Zhu, Feihong
collection PubMed
description CONTEXT: Cryptotanshinone (CT), a lipophilic compound extracted from roots of Salvia miltiorrhiza Bunge (Lamiaceae) (Danshen), has multiple properties in diseases, such as pulmonary fibrosis, lung cancer, and osteoarthritis. Our previous findings suggest that CT plays a protective role in cerebral stroke. However, the molecular mechanisms underlying CT protection in ischaemic stroke remain unclear. OBJECTIVE: This study examines the effect of CT on ischaemic stroke. MATERIALS AND METHODS: We used the middle cerebral artery occlusion (MCAO) rat (Sprague-Dawley rats, 200 ± 20 g, n = 5) model with a sham operation group was treated as negative control. MCAO rats were treated with 15 mg/kg CT using intragastric administration. Moreover, TGF-β (5 ng/mL) was used to treat MCAO rats as a positive control group. RESULTS: The 50% inhibitory concentration (IC(50)) of CT on CD4(+) cell damage was 485.1 μg/mL, and median effective concentration (EC(50)) was 485.1 μg/mL. CT attenuates the infarct region in the MCAO model. The percentage of CD4(+)CD25(+)FOXP3(+) Treg cells in the peripheral blood of the MCAO group was increased with CT treatment. The protein level of FOXP3 and the phosphorylation of STAT5 were recovered in the CD4(+)CD25(+) Treg cells of model group after treated with CT. Importantly, the effects of CT treatment were blocked by treatment with the inhibitor STAT5-IN-1 in CD4(+) T cells of the MCAO model. DISCUSSION AND CONCLUSION: Our findings not only enhance the understanding of the mechanisms underlying CT treatment, but also indicate its potential value as a promising agent in the treatment of ischaemic stroke. Further study will be valuable to examine the effects of CT on patients with ischaemic stroke.
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spelling pubmed-88716242022-02-25 Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model Zhu, Feihong Chen, Hehe Xu, Meifei Zhang, Xiajun Yu, Jing Pan, Yali Zhu, Weixin Pharm Biol Research Article CONTEXT: Cryptotanshinone (CT), a lipophilic compound extracted from roots of Salvia miltiorrhiza Bunge (Lamiaceae) (Danshen), has multiple properties in diseases, such as pulmonary fibrosis, lung cancer, and osteoarthritis. Our previous findings suggest that CT plays a protective role in cerebral stroke. However, the molecular mechanisms underlying CT protection in ischaemic stroke remain unclear. OBJECTIVE: This study examines the effect of CT on ischaemic stroke. MATERIALS AND METHODS: We used the middle cerebral artery occlusion (MCAO) rat (Sprague-Dawley rats, 200 ± 20 g, n = 5) model with a sham operation group was treated as negative control. MCAO rats were treated with 15 mg/kg CT using intragastric administration. Moreover, TGF-β (5 ng/mL) was used to treat MCAO rats as a positive control group. RESULTS: The 50% inhibitory concentration (IC(50)) of CT on CD4(+) cell damage was 485.1 μg/mL, and median effective concentration (EC(50)) was 485.1 μg/mL. CT attenuates the infarct region in the MCAO model. The percentage of CD4(+)CD25(+)FOXP3(+) Treg cells in the peripheral blood of the MCAO group was increased with CT treatment. The protein level of FOXP3 and the phosphorylation of STAT5 were recovered in the CD4(+)CD25(+) Treg cells of model group after treated with CT. Importantly, the effects of CT treatment were blocked by treatment with the inhibitor STAT5-IN-1 in CD4(+) T cells of the MCAO model. DISCUSSION AND CONCLUSION: Our findings not only enhance the understanding of the mechanisms underlying CT treatment, but also indicate its potential value as a promising agent in the treatment of ischaemic stroke. Further study will be valuable to examine the effects of CT on patients with ischaemic stroke. Taylor & Francis 2021-04-29 /pmc/articles/PMC8871624/ /pubmed/33915069 http://dx.doi.org/10.1080/13880209.2021.1914672 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhu, Feihong
Chen, Hehe
Xu, Meifei
Zhang, Xiajun
Yu, Jing
Pan, Yali
Zhu, Weixin
Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model
title Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model
title_full Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model
title_fullStr Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model
title_full_unstemmed Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model
title_short Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model
title_sort cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating stat5 in a rat model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871624/
https://www.ncbi.nlm.nih.gov/pubmed/33915069
http://dx.doi.org/10.1080/13880209.2021.1914672
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