Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L.

The cellulose synthase genes control the biosynthesis of cellulose in plants. Nonetheless, the gene family members of CesA have not been identified in the newly assembled genome of Gossypium hirsutum (AD1, HEBAU_NDM8). We identified 38 CesA genes in G. hirsutum (NDM8) and found that the protein sequ...

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Autores principales: Zhao, Ruolin, Cheng, Hailiang, Wang, Qiaolian, Lv, Limin, Zhang, Youping, Song, Guoli, Zuo, Dongyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871739/
https://www.ncbi.nlm.nih.gov/pubmed/35205337
http://dx.doi.org/10.3390/genes13020292
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author Zhao, Ruolin
Cheng, Hailiang
Wang, Qiaolian
Lv, Limin
Zhang, Youping
Song, Guoli
Zuo, Dongyun
author_facet Zhao, Ruolin
Cheng, Hailiang
Wang, Qiaolian
Lv, Limin
Zhang, Youping
Song, Guoli
Zuo, Dongyun
author_sort Zhao, Ruolin
collection PubMed
description The cellulose synthase genes control the biosynthesis of cellulose in plants. Nonetheless, the gene family members of CesA have not been identified in the newly assembled genome of Gossypium hirsutum (AD1, HEBAU_NDM8). We identified 38 CesA genes in G. hirsutum (NDM8) and found that the protein sequence of GhMCesA35 is 100% identical to CelA1 in a previous study. It is already known that CelA1 is involved in cellulose biosynthesis in vitro. However, the function of this gene in vivo has not been validated. In this study, we verified the function of GhMCesA35 in vivo based on overexpressed Arabidopsis thaliana. In addition, we found that it interacted with GhCesA7 through the yeast two-hybrid assay. This study provides new insights for studying the biological functions of CesA genes in G. hirsutum, thereby improving cotton fiber quality and yield.
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spelling pubmed-88717392022-02-25 Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L. Zhao, Ruolin Cheng, Hailiang Wang, Qiaolian Lv, Limin Zhang, Youping Song, Guoli Zuo, Dongyun Genes (Basel) Article The cellulose synthase genes control the biosynthesis of cellulose in plants. Nonetheless, the gene family members of CesA have not been identified in the newly assembled genome of Gossypium hirsutum (AD1, HEBAU_NDM8). We identified 38 CesA genes in G. hirsutum (NDM8) and found that the protein sequence of GhMCesA35 is 100% identical to CelA1 in a previous study. It is already known that CelA1 is involved in cellulose biosynthesis in vitro. However, the function of this gene in vivo has not been validated. In this study, we verified the function of GhMCesA35 in vivo based on overexpressed Arabidopsis thaliana. In addition, we found that it interacted with GhCesA7 through the yeast two-hybrid assay. This study provides new insights for studying the biological functions of CesA genes in G. hirsutum, thereby improving cotton fiber quality and yield. MDPI 2022-02-01 /pmc/articles/PMC8871739/ /pubmed/35205337 http://dx.doi.org/10.3390/genes13020292 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Ruolin
Cheng, Hailiang
Wang, Qiaolian
Lv, Limin
Zhang, Youping
Song, Guoli
Zuo, Dongyun
Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L.
title Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L.
title_full Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L.
title_fullStr Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L.
title_full_unstemmed Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L.
title_short Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L.
title_sort identification of the cesa subfamily and functional analysis of ghmcesa35 in gossypium hirsutum l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871739/
https://www.ncbi.nlm.nih.gov/pubmed/35205337
http://dx.doi.org/10.3390/genes13020292
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