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Expression Profiles of miR-22-5p and miR-142-3p Indicate Hashimoto’s Disease and Are related to Thyroid Antibodies

Hashimoto’s thyroiditis (HT) is the most prevalent autoimmune disorder of the thyroid (AITD) and characterized by the presence of circulating autoantibodies evoked by a, to date, not fully understood dysregulation of the immune system. Autoreactive lymphocytes and inflammatory processes in the thyro...

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Autores principales: Trummer, Olivia, Foessl, Ines, Schweighofer, Natascha, Arifi, Edi, Haudum, Christoph W., Reintar, Sharmaine, Pilz, Stefan, Theiler-Schwetz, Verena, Trummer, Christian, Zirlik, Andreas, Schmidt, Albrecht, Colantonio, Caterina, Kolesnik, Ewald, Verheyen, Nicolas, Pieber, Thomas R., Obermayer-Pietsch, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871926/
https://www.ncbi.nlm.nih.gov/pubmed/35205216
http://dx.doi.org/10.3390/genes13020171
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author Trummer, Olivia
Foessl, Ines
Schweighofer, Natascha
Arifi, Edi
Haudum, Christoph W.
Reintar, Sharmaine
Pilz, Stefan
Theiler-Schwetz, Verena
Trummer, Christian
Zirlik, Andreas
Schmidt, Albrecht
Colantonio, Caterina
Kolesnik, Ewald
Verheyen, Nicolas
Pieber, Thomas R.
Obermayer-Pietsch, Barbara
author_facet Trummer, Olivia
Foessl, Ines
Schweighofer, Natascha
Arifi, Edi
Haudum, Christoph W.
Reintar, Sharmaine
Pilz, Stefan
Theiler-Schwetz, Verena
Trummer, Christian
Zirlik, Andreas
Schmidt, Albrecht
Colantonio, Caterina
Kolesnik, Ewald
Verheyen, Nicolas
Pieber, Thomas R.
Obermayer-Pietsch, Barbara
author_sort Trummer, Olivia
collection PubMed
description Hashimoto’s thyroiditis (HT) is the most prevalent autoimmune disorder of the thyroid (AITD) and characterized by the presence of circulating autoantibodies evoked by a, to date, not fully understood dysregulation of the immune system. Autoreactive lymphocytes and inflammatory processes in the thyroid gland can impair or enhance thyroid hormone secretion. MicroRNAs (miRNAs) are small noncoding RNAs, which can play a pivotal role in immune functions and the development of autoimmunity. The aim of the present study was to evaluate whether the expression of 9 selected miRNAs related to immunological functions differ in patients with HT compared to healthy controls. MiRNA profiles were analysed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 24 patients with HT and 17 healthy controls. Systemic expressions of miR-21-5p, miR-22-3p, miR-22-5p, miR-142-3p, miR-146a-5p, miR-301-3p and miR-451 were significantly upregulated in patients with HT (p ≤ 0.01) and were suitable to discriminate between HT and healthy controls in AUC analysis. Altered expressions of miR-22-5p and miR-142-3p were associated with higher levels of thyroid antibodies, suggesting their contribution to the pathogenesis of HT.
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spelling pubmed-88719262022-02-25 Expression Profiles of miR-22-5p and miR-142-3p Indicate Hashimoto’s Disease and Are related to Thyroid Antibodies Trummer, Olivia Foessl, Ines Schweighofer, Natascha Arifi, Edi Haudum, Christoph W. Reintar, Sharmaine Pilz, Stefan Theiler-Schwetz, Verena Trummer, Christian Zirlik, Andreas Schmidt, Albrecht Colantonio, Caterina Kolesnik, Ewald Verheyen, Nicolas Pieber, Thomas R. Obermayer-Pietsch, Barbara Genes (Basel) Article Hashimoto’s thyroiditis (HT) is the most prevalent autoimmune disorder of the thyroid (AITD) and characterized by the presence of circulating autoantibodies evoked by a, to date, not fully understood dysregulation of the immune system. Autoreactive lymphocytes and inflammatory processes in the thyroid gland can impair or enhance thyroid hormone secretion. MicroRNAs (miRNAs) are small noncoding RNAs, which can play a pivotal role in immune functions and the development of autoimmunity. The aim of the present study was to evaluate whether the expression of 9 selected miRNAs related to immunological functions differ in patients with HT compared to healthy controls. MiRNA profiles were analysed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 24 patients with HT and 17 healthy controls. Systemic expressions of miR-21-5p, miR-22-3p, miR-22-5p, miR-142-3p, miR-146a-5p, miR-301-3p and miR-451 were significantly upregulated in patients with HT (p ≤ 0.01) and were suitable to discriminate between HT and healthy controls in AUC analysis. Altered expressions of miR-22-5p and miR-142-3p were associated with higher levels of thyroid antibodies, suggesting their contribution to the pathogenesis of HT. MDPI 2022-01-19 /pmc/articles/PMC8871926/ /pubmed/35205216 http://dx.doi.org/10.3390/genes13020171 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trummer, Olivia
Foessl, Ines
Schweighofer, Natascha
Arifi, Edi
Haudum, Christoph W.
Reintar, Sharmaine
Pilz, Stefan
Theiler-Schwetz, Verena
Trummer, Christian
Zirlik, Andreas
Schmidt, Albrecht
Colantonio, Caterina
Kolesnik, Ewald
Verheyen, Nicolas
Pieber, Thomas R.
Obermayer-Pietsch, Barbara
Expression Profiles of miR-22-5p and miR-142-3p Indicate Hashimoto’s Disease and Are related to Thyroid Antibodies
title Expression Profiles of miR-22-5p and miR-142-3p Indicate Hashimoto’s Disease and Are related to Thyroid Antibodies
title_full Expression Profiles of miR-22-5p and miR-142-3p Indicate Hashimoto’s Disease and Are related to Thyroid Antibodies
title_fullStr Expression Profiles of miR-22-5p and miR-142-3p Indicate Hashimoto’s Disease and Are related to Thyroid Antibodies
title_full_unstemmed Expression Profiles of miR-22-5p and miR-142-3p Indicate Hashimoto’s Disease and Are related to Thyroid Antibodies
title_short Expression Profiles of miR-22-5p and miR-142-3p Indicate Hashimoto’s Disease and Are related to Thyroid Antibodies
title_sort expression profiles of mir-22-5p and mir-142-3p indicate hashimoto’s disease and are related to thyroid antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8871926/
https://www.ncbi.nlm.nih.gov/pubmed/35205216
http://dx.doi.org/10.3390/genes13020171
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