Cargando…

Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice

The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model...

Descripción completa

Detalles Bibliográficos
Autores principales: Xue, Ruizhi, Lin, Wenfeng, Fujita, Hirofumi, Sun, Jingkai, Kinoshita, Rie, Ochiai, Kazuhiko, Futami, Junichiro, Watanabe, Masami, Ohuchi, Hideyo, Sakaguchi, Masakiyo, Tang, Zhengyan, Huang, Peng, Nasu, Yasutomo, Kumon, Hiromi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872165/
https://www.ncbi.nlm.nih.gov/pubmed/35205329
http://dx.doi.org/10.3390/genes13020285
_version_ 1784657169459707904
author Xue, Ruizhi
Lin, Wenfeng
Fujita, Hirofumi
Sun, Jingkai
Kinoshita, Rie
Ochiai, Kazuhiko
Futami, Junichiro
Watanabe, Masami
Ohuchi, Hideyo
Sakaguchi, Masakiyo
Tang, Zhengyan
Huang, Peng
Nasu, Yasutomo
Kumon, Hiromi
author_facet Xue, Ruizhi
Lin, Wenfeng
Fujita, Hirofumi
Sun, Jingkai
Kinoshita, Rie
Ochiai, Kazuhiko
Futami, Junichiro
Watanabe, Masami
Ohuchi, Hideyo
Sakaguchi, Masakiyo
Tang, Zhengyan
Huang, Peng
Nasu, Yasutomo
Kumon, Hiromi
author_sort Xue, Ruizhi
collection PubMed
description The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model. By examining testicular sections and investigating the sperm characteristics (count, vitality and motility) and ultrastructure, we compared the reproductive features between Dkk3/REIC-KO and wild-type (WT) male mice. To further explore the underlying molecular mechanism, we performed RNA sequencing (RNA-seq) analysis of testicular tissues. Our results showed that spermiation failure existed in seminiferous tubules of Dkk3/REIC-KO mice, and sperm from Dkk3/REIC-KO mice exhibited inferior motility (44.09 ± 8.12% vs. 23.26 ± 10.02%, p < 0.01). The Ultrastructure examination revealed defects in the sperm fibrous sheath of KO mice. Although the average count of Dkk3/REIC-KO epididymal sperm was less than that of the wild-types (9.30 ± 0.69 vs. 8.27 ± 0.87, ×10(6)), neither the gap (p > 0.05) nor the difference in the sperm vitality rate (72.83 ± 1.55% vs. 72.50 ± 0.71%, p > 0.05) were statistically significant. The RNA-seq and GO (Gene Oncology) enrichment results indicated that the differential genes were significantly enriched in the GO terms of cytoskeleton function, cAMP signaling and calcium ion binding. Collectively, our research demonstrates that Dkk3/REIC is involved in the process of spermiation, fibrous sheath integrity maintenance and sperm motility of mice.
format Online
Article
Text
id pubmed-8872165
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88721652022-02-25 Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice Xue, Ruizhi Lin, Wenfeng Fujita, Hirofumi Sun, Jingkai Kinoshita, Rie Ochiai, Kazuhiko Futami, Junichiro Watanabe, Masami Ohuchi, Hideyo Sakaguchi, Masakiyo Tang, Zhengyan Huang, Peng Nasu, Yasutomo Kumon, Hiromi Genes (Basel) Article The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model. By examining testicular sections and investigating the sperm characteristics (count, vitality and motility) and ultrastructure, we compared the reproductive features between Dkk3/REIC-KO and wild-type (WT) male mice. To further explore the underlying molecular mechanism, we performed RNA sequencing (RNA-seq) analysis of testicular tissues. Our results showed that spermiation failure existed in seminiferous tubules of Dkk3/REIC-KO mice, and sperm from Dkk3/REIC-KO mice exhibited inferior motility (44.09 ± 8.12% vs. 23.26 ± 10.02%, p < 0.01). The Ultrastructure examination revealed defects in the sperm fibrous sheath of KO mice. Although the average count of Dkk3/REIC-KO epididymal sperm was less than that of the wild-types (9.30 ± 0.69 vs. 8.27 ± 0.87, ×10(6)), neither the gap (p > 0.05) nor the difference in the sperm vitality rate (72.83 ± 1.55% vs. 72.50 ± 0.71%, p > 0.05) were statistically significant. The RNA-seq and GO (Gene Oncology) enrichment results indicated that the differential genes were significantly enriched in the GO terms of cytoskeleton function, cAMP signaling and calcium ion binding. Collectively, our research demonstrates that Dkk3/REIC is involved in the process of spermiation, fibrous sheath integrity maintenance and sperm motility of mice. MDPI 2022-01-31 /pmc/articles/PMC8872165/ /pubmed/35205329 http://dx.doi.org/10.3390/genes13020285 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xue, Ruizhi
Lin, Wenfeng
Fujita, Hirofumi
Sun, Jingkai
Kinoshita, Rie
Ochiai, Kazuhiko
Futami, Junichiro
Watanabe, Masami
Ohuchi, Hideyo
Sakaguchi, Masakiyo
Tang, Zhengyan
Huang, Peng
Nasu, Yasutomo
Kumon, Hiromi
Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice
title Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice
title_full Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice
title_fullStr Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice
title_full_unstemmed Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice
title_short Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice
title_sort dkk3/reic deficiency impairs spermiation, sperm fibrous sheath integrity and the sperm motility of mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872165/
https://www.ncbi.nlm.nih.gov/pubmed/35205329
http://dx.doi.org/10.3390/genes13020285
work_keys_str_mv AT xueruizhi dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT linwenfeng dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT fujitahirofumi dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT sunjingkai dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT kinoshitarie dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT ochiaikazuhiko dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT futamijunichiro dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT watanabemasami dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT ohuchihideyo dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT sakaguchimasakiyo dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT tangzhengyan dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT huangpeng dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT nasuyasutomo dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice
AT kumonhiromi dkk3reicdeficiencyimpairsspermiationspermfibroussheathintegrityandthespermmotilityofmice