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“Silicon-On-Insulator”-Based Nanosensor for the Revelation of MicroRNA Markers of Autism

MicroRNAs (miRNAs), which represent short (20 to 22 nt) non-coding RNAs, were found to play a direct role in the development of autism in children. Herein, a highly sensitive “silicon-on-insulator”-based nanosensor (SOI-NS) has been developed for the revelation of autism-associated miRNAs. This SOI-...

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Autores principales: Ivanov, Yuri D., Malsagova, Kristina A., Goldaeva, Kristina V., Pleshakova, Tatyana O., Shumov, Ivan D., Galiullin, Rafael A., Kapustina, Svetlana I., Iourov, Ivan Y., Vorsanova, Svetlana G., Ryabtsev, Stepan V., Popov, Vladimir P., Archakov, Alexander I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872218/
https://www.ncbi.nlm.nih.gov/pubmed/35205244
http://dx.doi.org/10.3390/genes13020199
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author Ivanov, Yuri D.
Malsagova, Kristina A.
Goldaeva, Kristina V.
Pleshakova, Tatyana O.
Shumov, Ivan D.
Galiullin, Rafael A.
Kapustina, Svetlana I.
Iourov, Ivan Y.
Vorsanova, Svetlana G.
Ryabtsev, Stepan V.
Popov, Vladimir P.
Archakov, Alexander I.
author_facet Ivanov, Yuri D.
Malsagova, Kristina A.
Goldaeva, Kristina V.
Pleshakova, Tatyana O.
Shumov, Ivan D.
Galiullin, Rafael A.
Kapustina, Svetlana I.
Iourov, Ivan Y.
Vorsanova, Svetlana G.
Ryabtsev, Stepan V.
Popov, Vladimir P.
Archakov, Alexander I.
author_sort Ivanov, Yuri D.
collection PubMed
description MicroRNAs (miRNAs), which represent short (20 to 22 nt) non-coding RNAs, were found to play a direct role in the development of autism in children. Herein, a highly sensitive “silicon-on-insulator”-based nanosensor (SOI-NS) has been developed for the revelation of autism-associated miRNAs. This SOI-NS comprises an array of nanowire sensor structures fabricated by complementary metal–oxide–semiconductor (CMOS)-compatible technology, gas-phase etching, and nanolithography. In our experiments described herein, we demonstrate the revelation of ASD-associated miRNAs in human plasma with the SOI-NS, whose sensor elements were sensitized with oligonucleotide probes. In order to determine the concentration sensitivity of the SOI-NS, experiments on the detection of synthetic DNA analogues of autism-associated miRNAs in purified buffer were performed. The lower limit of miRNA detection attained in our experiments amounted to 10(−17) M.
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spelling pubmed-88722182022-02-25 “Silicon-On-Insulator”-Based Nanosensor for the Revelation of MicroRNA Markers of Autism Ivanov, Yuri D. Malsagova, Kristina A. Goldaeva, Kristina V. Pleshakova, Tatyana O. Shumov, Ivan D. Galiullin, Rafael A. Kapustina, Svetlana I. Iourov, Ivan Y. Vorsanova, Svetlana G. Ryabtsev, Stepan V. Popov, Vladimir P. Archakov, Alexander I. Genes (Basel) Article MicroRNAs (miRNAs), which represent short (20 to 22 nt) non-coding RNAs, were found to play a direct role in the development of autism in children. Herein, a highly sensitive “silicon-on-insulator”-based nanosensor (SOI-NS) has been developed for the revelation of autism-associated miRNAs. This SOI-NS comprises an array of nanowire sensor structures fabricated by complementary metal–oxide–semiconductor (CMOS)-compatible technology, gas-phase etching, and nanolithography. In our experiments described herein, we demonstrate the revelation of ASD-associated miRNAs in human plasma with the SOI-NS, whose sensor elements were sensitized with oligonucleotide probes. In order to determine the concentration sensitivity of the SOI-NS, experiments on the detection of synthetic DNA analogues of autism-associated miRNAs in purified buffer were performed. The lower limit of miRNA detection attained in our experiments amounted to 10(−17) M. MDPI 2022-01-22 /pmc/articles/PMC8872218/ /pubmed/35205244 http://dx.doi.org/10.3390/genes13020199 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ivanov, Yuri D.
Malsagova, Kristina A.
Goldaeva, Kristina V.
Pleshakova, Tatyana O.
Shumov, Ivan D.
Galiullin, Rafael A.
Kapustina, Svetlana I.
Iourov, Ivan Y.
Vorsanova, Svetlana G.
Ryabtsev, Stepan V.
Popov, Vladimir P.
Archakov, Alexander I.
“Silicon-On-Insulator”-Based Nanosensor for the Revelation of MicroRNA Markers of Autism
title “Silicon-On-Insulator”-Based Nanosensor for the Revelation of MicroRNA Markers of Autism
title_full “Silicon-On-Insulator”-Based Nanosensor for the Revelation of MicroRNA Markers of Autism
title_fullStr “Silicon-On-Insulator”-Based Nanosensor for the Revelation of MicroRNA Markers of Autism
title_full_unstemmed “Silicon-On-Insulator”-Based Nanosensor for the Revelation of MicroRNA Markers of Autism
title_short “Silicon-On-Insulator”-Based Nanosensor for the Revelation of MicroRNA Markers of Autism
title_sort “silicon-on-insulator”-based nanosensor for the revelation of microrna markers of autism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872218/
https://www.ncbi.nlm.nih.gov/pubmed/35205244
http://dx.doi.org/10.3390/genes13020199
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