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Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells

FAM49B/Fam49b is a member of the Fam49 (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its important function of regulating RAC1-d...

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Autores principales: Chen, Yijian, Jiang, Yuyan, Lao, Jihui, Zhou, Yankuan, Su, Lida, Huang, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872254/
https://www.ncbi.nlm.nih.gov/pubmed/35205432
http://dx.doi.org/10.3390/genes13020388
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author Chen, Yijian
Jiang, Yuyan
Lao, Jihui
Zhou, Yankuan
Su, Lida
Huang, Xiao
author_facet Chen, Yijian
Jiang, Yuyan
Lao, Jihui
Zhou, Yankuan
Su, Lida
Huang, Xiao
author_sort Chen, Yijian
collection PubMed
description FAM49B/Fam49b is a member of the Fam49 (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its important function of regulating RAC1-driven cytoskeleton remolding. In this study, to further investigate its functions and mechanisms affecting cell behaviors, HEK293T cells (where FAM49B is highly expressed) were used to establish a FAM49B knockout cell line by CRISPR/Cas9 genome editing technology. Our data have clearly revealed that there are triple alleles of FAM49B in the genome of HEK293T cells. Meanwhile, the proliferation deficiency of the FAM49B KO HEK293T cell line and the significantly changed cell proliferation related gene expression profiles, such as CCND1, have been uncovered. At the same time, the existence of isoform 3 has been confirmed in HEK293T cells. Our studies have suggested that FAM49B may also affect cell proliferation via Cyclins, besides its influence on the cytoskeleton.
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spelling pubmed-88722542022-02-25 Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells Chen, Yijian Jiang, Yuyan Lao, Jihui Zhou, Yankuan Su, Lida Huang, Xiao Genes (Basel) Article FAM49B/Fam49b is a member of the Fam49 (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its important function of regulating RAC1-driven cytoskeleton remolding. In this study, to further investigate its functions and mechanisms affecting cell behaviors, HEK293T cells (where FAM49B is highly expressed) were used to establish a FAM49B knockout cell line by CRISPR/Cas9 genome editing technology. Our data have clearly revealed that there are triple alleles of FAM49B in the genome of HEK293T cells. Meanwhile, the proliferation deficiency of the FAM49B KO HEK293T cell line and the significantly changed cell proliferation related gene expression profiles, such as CCND1, have been uncovered. At the same time, the existence of isoform 3 has been confirmed in HEK293T cells. Our studies have suggested that FAM49B may also affect cell proliferation via Cyclins, besides its influence on the cytoskeleton. MDPI 2022-02-21 /pmc/articles/PMC8872254/ /pubmed/35205432 http://dx.doi.org/10.3390/genes13020388 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yijian
Jiang, Yuyan
Lao, Jihui
Zhou, Yankuan
Su, Lida
Huang, Xiao
Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells
title Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells
title_full Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells
title_fullStr Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells
title_full_unstemmed Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells
title_short Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells
title_sort characterization and functional study of fam49b reveals its effect on cell proliferation in hek293t cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872254/
https://www.ncbi.nlm.nih.gov/pubmed/35205432
http://dx.doi.org/10.3390/genes13020388
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