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Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells

BACKGROUND: Lung cancer in men and women is considered the leading cause for cancer-related mortality worldwide. Anti-cancer peptides represent a potential untapped reservoir of effective cancer therapy. METHODOLOGY: Box-Behnken response surface design was applied for formulating Alendronate sodium...

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Autores principales: Alhakamy, Nabil A., Okbazghi, Solomon Z., A. Alfaleh, Mohamed, H. Abdulaal, Wesam, Bakhaidar, Rana B., Alselami, Mohammed O., Zahrani, Majed AL, Alqarni, Hani M., F. Alghaith, Adel, Alshehri, Sultan, Badr-Eldin, Shaimaa M., Aldawsari, Hibah M., Al-hejaili, Omar D., Aldhabi, Bander M., Mahdi, Wael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872391/
https://www.ncbi.nlm.nih.gov/pubmed/35202419
http://dx.doi.org/10.1371/journal.pone.0264093
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author Alhakamy, Nabil A.
Okbazghi, Solomon Z.
A. Alfaleh, Mohamed
H. Abdulaal, Wesam
Bakhaidar, Rana B.
Alselami, Mohammed O.
Zahrani, Majed AL
Alqarni, Hani M.
F. Alghaith, Adel
Alshehri, Sultan
Badr-Eldin, Shaimaa M.
Aldawsari, Hibah M.
Al-hejaili, Omar D.
Aldhabi, Bander M.
Mahdi, Wael A.
author_facet Alhakamy, Nabil A.
Okbazghi, Solomon Z.
A. Alfaleh, Mohamed
H. Abdulaal, Wesam
Bakhaidar, Rana B.
Alselami, Mohammed O.
Zahrani, Majed AL
Alqarni, Hani M.
F. Alghaith, Adel
Alshehri, Sultan
Badr-Eldin, Shaimaa M.
Aldawsari, Hibah M.
Al-hejaili, Omar D.
Aldhabi, Bander M.
Mahdi, Wael A.
author_sort Alhakamy, Nabil A.
collection PubMed
description BACKGROUND: Lung cancer in men and women is considered the leading cause for cancer-related mortality worldwide. Anti-cancer peptides represent a potential untapped reservoir of effective cancer therapy. METHODOLOGY: Box-Behnken response surface design was applied for formulating Alendronate sodium (ALS)-mastoparan peptide (MP) nanoconjugates using Design-Expert software. The optimization process aimed at minimizing the size of the prepared ALS-MP nanoconjugates. ALS-MP nanoconjugates’ particle size, encapsulation efficiency and the release profile were determined. Cytotoxicity, cell cycle, annexin V staining and caspase 3 analyses on A549 cells were carried out for the optimized formula. RESULTS: The results revealed that the optimized formula was of 134.91±5.1 nm particle size. The novel ALS-MP demonstrated the lowest IC50 (1.3 ± 0.34 μM) in comparison to ALS-Raw (37.6 ± 1.79 μM). Thus, the results indicated that when optimized ALS-MP nanoconjugate was used, the IC50 of ALS was also reduced by half. Cell cycle analysis demonstrated a significantly higher percentage of cells in the G2-M phase following the treatment with optimized ALS-MP nanoconjugates. CONCLUSION: The optimized ALS-MP formula had significantly improved the parameters related to the cytotoxic activity towards A549 cells, compared to control, MP and ALS-Raw.
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spelling pubmed-88723912022-02-25 Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells Alhakamy, Nabil A. Okbazghi, Solomon Z. A. Alfaleh, Mohamed H. Abdulaal, Wesam Bakhaidar, Rana B. Alselami, Mohammed O. Zahrani, Majed AL Alqarni, Hani M. F. Alghaith, Adel Alshehri, Sultan Badr-Eldin, Shaimaa M. Aldawsari, Hibah M. Al-hejaili, Omar D. Aldhabi, Bander M. Mahdi, Wael A. PLoS One Research Article BACKGROUND: Lung cancer in men and women is considered the leading cause for cancer-related mortality worldwide. Anti-cancer peptides represent a potential untapped reservoir of effective cancer therapy. METHODOLOGY: Box-Behnken response surface design was applied for formulating Alendronate sodium (ALS)-mastoparan peptide (MP) nanoconjugates using Design-Expert software. The optimization process aimed at minimizing the size of the prepared ALS-MP nanoconjugates. ALS-MP nanoconjugates’ particle size, encapsulation efficiency and the release profile were determined. Cytotoxicity, cell cycle, annexin V staining and caspase 3 analyses on A549 cells were carried out for the optimized formula. RESULTS: The results revealed that the optimized formula was of 134.91±5.1 nm particle size. The novel ALS-MP demonstrated the lowest IC50 (1.3 ± 0.34 μM) in comparison to ALS-Raw (37.6 ± 1.79 μM). Thus, the results indicated that when optimized ALS-MP nanoconjugate was used, the IC50 of ALS was also reduced by half. Cell cycle analysis demonstrated a significantly higher percentage of cells in the G2-M phase following the treatment with optimized ALS-MP nanoconjugates. CONCLUSION: The optimized ALS-MP formula had significantly improved the parameters related to the cytotoxic activity towards A549 cells, compared to control, MP and ALS-Raw. Public Library of Science 2022-02-24 /pmc/articles/PMC8872391/ /pubmed/35202419 http://dx.doi.org/10.1371/journal.pone.0264093 Text en © 2022 Alhakamy et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Alhakamy, Nabil A.
Okbazghi, Solomon Z.
A. Alfaleh, Mohamed
H. Abdulaal, Wesam
Bakhaidar, Rana B.
Alselami, Mohammed O.
Zahrani, Majed AL
Alqarni, Hani M.
F. Alghaith, Adel
Alshehri, Sultan
Badr-Eldin, Shaimaa M.
Aldawsari, Hibah M.
Al-hejaili, Omar D.
Aldhabi, Bander M.
Mahdi, Wael A.
Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells
title Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells
title_full Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells
title_fullStr Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells
title_full_unstemmed Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells
title_short Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells
title_sort wasp venom peptide improves the proapoptotic activity of alendronate sodium in a549 lung cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872391/
https://www.ncbi.nlm.nih.gov/pubmed/35202419
http://dx.doi.org/10.1371/journal.pone.0264093
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