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Genome-Wide Association Study of Root System Architecture in Maize

Roots are important plant organs for the absorption of water and nutrients. To date, there have been few genome-wide association studies of maize root system architecture (RSA) in the field. The genetic basis of maize RSA is poorly understood, and the maize RSA-related genes that have been cloned ar...

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Autores principales: Wu, Bin, Ren, Wei, Zhao, Longfei, Li, Qiang, Sun, Jiazheng, Chen, Fanjun, Pan, Qingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872597/
https://www.ncbi.nlm.nih.gov/pubmed/35205226
http://dx.doi.org/10.3390/genes13020181
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author Wu, Bin
Ren, Wei
Zhao, Longfei
Li, Qiang
Sun, Jiazheng
Chen, Fanjun
Pan, Qingchun
author_facet Wu, Bin
Ren, Wei
Zhao, Longfei
Li, Qiang
Sun, Jiazheng
Chen, Fanjun
Pan, Qingchun
author_sort Wu, Bin
collection PubMed
description Roots are important plant organs for the absorption of water and nutrients. To date, there have been few genome-wide association studies of maize root system architecture (RSA) in the field. The genetic basis of maize RSA is poorly understood, and the maize RSA-related genes that have been cloned are very limited. Here, 421 maize inbred lines of an association panel were planted to measure the root systems at the maturity stage, and a genome-wide association study was performed. There was a strong correlation among eight RSA traits, and the RSA traits were highly correlated with the aboveground plant architecture traits (e.g., plant height and ear leaf length, r = 0.13–0.25, p < 0.05). The RSA traits of the stiff stalk subgroup (SS) showed lower values than those of the non-stiff stalk subgroup (NSS) and tropical/subtropical subgroup (TST). Using the RSA traits, the genome-wide association study identified 63 SNPs and 189 candidate genes. Among them, nine candidate genes co-localized between RSA and aboveground architecture traits. A further co-expression analysis identified 88 candidate genes having high confidence levels. Furthermore, we identified four highly reliable RSA candidate genes, GRMZM2G099797, GRMZM2G354338, GRMZM2G085042, and GRMZM5G812926. This research provides theoretical support for the genetic improvement of maize root systems, and it identified candidate genes that may act as genetic resources for breeding.
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spelling pubmed-88725972022-02-25 Genome-Wide Association Study of Root System Architecture in Maize Wu, Bin Ren, Wei Zhao, Longfei Li, Qiang Sun, Jiazheng Chen, Fanjun Pan, Qingchun Genes (Basel) Article Roots are important plant organs for the absorption of water and nutrients. To date, there have been few genome-wide association studies of maize root system architecture (RSA) in the field. The genetic basis of maize RSA is poorly understood, and the maize RSA-related genes that have been cloned are very limited. Here, 421 maize inbred lines of an association panel were planted to measure the root systems at the maturity stage, and a genome-wide association study was performed. There was a strong correlation among eight RSA traits, and the RSA traits were highly correlated with the aboveground plant architecture traits (e.g., plant height and ear leaf length, r = 0.13–0.25, p < 0.05). The RSA traits of the stiff stalk subgroup (SS) showed lower values than those of the non-stiff stalk subgroup (NSS) and tropical/subtropical subgroup (TST). Using the RSA traits, the genome-wide association study identified 63 SNPs and 189 candidate genes. Among them, nine candidate genes co-localized between RSA and aboveground architecture traits. A further co-expression analysis identified 88 candidate genes having high confidence levels. Furthermore, we identified four highly reliable RSA candidate genes, GRMZM2G099797, GRMZM2G354338, GRMZM2G085042, and GRMZM5G812926. This research provides theoretical support for the genetic improvement of maize root systems, and it identified candidate genes that may act as genetic resources for breeding. MDPI 2022-01-28 /pmc/articles/PMC8872597/ /pubmed/35205226 http://dx.doi.org/10.3390/genes13020181 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Bin
Ren, Wei
Zhao, Longfei
Li, Qiang
Sun, Jiazheng
Chen, Fanjun
Pan, Qingchun
Genome-Wide Association Study of Root System Architecture in Maize
title Genome-Wide Association Study of Root System Architecture in Maize
title_full Genome-Wide Association Study of Root System Architecture in Maize
title_fullStr Genome-Wide Association Study of Root System Architecture in Maize
title_full_unstemmed Genome-Wide Association Study of Root System Architecture in Maize
title_short Genome-Wide Association Study of Root System Architecture in Maize
title_sort genome-wide association study of root system architecture in maize
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872597/
https://www.ncbi.nlm.nih.gov/pubmed/35205226
http://dx.doi.org/10.3390/genes13020181
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