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Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines

Among natural macromolecules, the polyphenol extract from Annurca flesh (AFPE) apple could play a potential therapeutic role for a large spectrum of human cancer also by exerting antioxidant properties. Thyroid cancer is a common neoplasia in women, and it is in general responsive to treatments alth...

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Autores principales: Orlandella, Francesca Maria, Mirabelli, Peppino, De Stefano, Anna Elisa, Iervolino, Paola Lucia Chiara, Luciano, Neila, D'Angelo, Stefania, Salvatore, Giuliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872649/
https://www.ncbi.nlm.nih.gov/pubmed/35222800
http://dx.doi.org/10.1155/2022/6268755
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author Orlandella, Francesca Maria
Mirabelli, Peppino
De Stefano, Anna Elisa
Iervolino, Paola Lucia Chiara
Luciano, Neila
D'Angelo, Stefania
Salvatore, Giuliana
author_facet Orlandella, Francesca Maria
Mirabelli, Peppino
De Stefano, Anna Elisa
Iervolino, Paola Lucia Chiara
Luciano, Neila
D'Angelo, Stefania
Salvatore, Giuliana
author_sort Orlandella, Francesca Maria
collection PubMed
description Among natural macromolecules, the polyphenol extract from Annurca flesh (AFPE) apple could play a potential therapeutic role for a large spectrum of human cancer also by exerting antioxidant properties. Thyroid cancer is a common neoplasia in women, and it is in general responsive to treatments although patients may relapse and metastasize or therapy-related side effects could occur. In this study, we explored the effects of AFPE on papillary (TPC-1) and anaplastic (CAL62) thyroid cancer cell line proliferation and viability. We found that AFPE exposure induced a reduction of cell proliferation and cell viability in dose-dependent manner. The effect was associated with the reduction of phosphorylation of Rb protein. To study the mechanisms underlying the biological effects of AFPE treatment in thyroid cancer cells, we investigated the modulation of miRNA (miR) expression. We found that AFPE treatment increased the expression of the miR-141, miR-145, miR-200a-5p, miR-425, and miR-551b-5p. Additionally, since natural polyphenols could exert their beneficial effects through the antioxidant properties, we investigated this aspect, and we found that AFPE treatment reduced the production of reactive oxygen species (ROS) in CAL62 cells. Moreover, AFPE pretreatment protects against hydrogen peroxide-induced oxidative stress in thyroid cancer cell lines. Taken together, our findings suggest that AFPE, by acting at micromolar concentration in thyroid cancer cell lines, may be considered a promising adjuvant natural agent for thyroid cancer treatment approach.
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spelling pubmed-88726492022-02-25 Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines Orlandella, Francesca Maria Mirabelli, Peppino De Stefano, Anna Elisa Iervolino, Paola Lucia Chiara Luciano, Neila D'Angelo, Stefania Salvatore, Giuliana Oxid Med Cell Longev Research Article Among natural macromolecules, the polyphenol extract from Annurca flesh (AFPE) apple could play a potential therapeutic role for a large spectrum of human cancer also by exerting antioxidant properties. Thyroid cancer is a common neoplasia in women, and it is in general responsive to treatments although patients may relapse and metastasize or therapy-related side effects could occur. In this study, we explored the effects of AFPE on papillary (TPC-1) and anaplastic (CAL62) thyroid cancer cell line proliferation and viability. We found that AFPE exposure induced a reduction of cell proliferation and cell viability in dose-dependent manner. The effect was associated with the reduction of phosphorylation of Rb protein. To study the mechanisms underlying the biological effects of AFPE treatment in thyroid cancer cells, we investigated the modulation of miRNA (miR) expression. We found that AFPE treatment increased the expression of the miR-141, miR-145, miR-200a-5p, miR-425, and miR-551b-5p. Additionally, since natural polyphenols could exert their beneficial effects through the antioxidant properties, we investigated this aspect, and we found that AFPE treatment reduced the production of reactive oxygen species (ROS) in CAL62 cells. Moreover, AFPE pretreatment protects against hydrogen peroxide-induced oxidative stress in thyroid cancer cell lines. Taken together, our findings suggest that AFPE, by acting at micromolar concentration in thyroid cancer cell lines, may be considered a promising adjuvant natural agent for thyroid cancer treatment approach. Hindawi 2022-02-17 /pmc/articles/PMC8872649/ /pubmed/35222800 http://dx.doi.org/10.1155/2022/6268755 Text en Copyright © 2022 Francesca Maria Orlandella et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Orlandella, Francesca Maria
Mirabelli, Peppino
De Stefano, Anna Elisa
Iervolino, Paola Lucia Chiara
Luciano, Neila
D'Angelo, Stefania
Salvatore, Giuliana
Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines
title Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines
title_full Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines
title_fullStr Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines
title_full_unstemmed Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines
title_short Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines
title_sort effects of annurca flesh apple polyphenols in human thyroid cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872649/
https://www.ncbi.nlm.nih.gov/pubmed/35222800
http://dx.doi.org/10.1155/2022/6268755
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