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Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines
Among natural macromolecules, the polyphenol extract from Annurca flesh (AFPE) apple could play a potential therapeutic role for a large spectrum of human cancer also by exerting antioxidant properties. Thyroid cancer is a common neoplasia in women, and it is in general responsive to treatments alth...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872649/ https://www.ncbi.nlm.nih.gov/pubmed/35222800 http://dx.doi.org/10.1155/2022/6268755 |
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author | Orlandella, Francesca Maria Mirabelli, Peppino De Stefano, Anna Elisa Iervolino, Paola Lucia Chiara Luciano, Neila D'Angelo, Stefania Salvatore, Giuliana |
author_facet | Orlandella, Francesca Maria Mirabelli, Peppino De Stefano, Anna Elisa Iervolino, Paola Lucia Chiara Luciano, Neila D'Angelo, Stefania Salvatore, Giuliana |
author_sort | Orlandella, Francesca Maria |
collection | PubMed |
description | Among natural macromolecules, the polyphenol extract from Annurca flesh (AFPE) apple could play a potential therapeutic role for a large spectrum of human cancer also by exerting antioxidant properties. Thyroid cancer is a common neoplasia in women, and it is in general responsive to treatments although patients may relapse and metastasize or therapy-related side effects could occur. In this study, we explored the effects of AFPE on papillary (TPC-1) and anaplastic (CAL62) thyroid cancer cell line proliferation and viability. We found that AFPE exposure induced a reduction of cell proliferation and cell viability in dose-dependent manner. The effect was associated with the reduction of phosphorylation of Rb protein. To study the mechanisms underlying the biological effects of AFPE treatment in thyroid cancer cells, we investigated the modulation of miRNA (miR) expression. We found that AFPE treatment increased the expression of the miR-141, miR-145, miR-200a-5p, miR-425, and miR-551b-5p. Additionally, since natural polyphenols could exert their beneficial effects through the antioxidant properties, we investigated this aspect, and we found that AFPE treatment reduced the production of reactive oxygen species (ROS) in CAL62 cells. Moreover, AFPE pretreatment protects against hydrogen peroxide-induced oxidative stress in thyroid cancer cell lines. Taken together, our findings suggest that AFPE, by acting at micromolar concentration in thyroid cancer cell lines, may be considered a promising adjuvant natural agent for thyroid cancer treatment approach. |
format | Online Article Text |
id | pubmed-8872649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-88726492022-02-25 Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines Orlandella, Francesca Maria Mirabelli, Peppino De Stefano, Anna Elisa Iervolino, Paola Lucia Chiara Luciano, Neila D'Angelo, Stefania Salvatore, Giuliana Oxid Med Cell Longev Research Article Among natural macromolecules, the polyphenol extract from Annurca flesh (AFPE) apple could play a potential therapeutic role for a large spectrum of human cancer also by exerting antioxidant properties. Thyroid cancer is a common neoplasia in women, and it is in general responsive to treatments although patients may relapse and metastasize or therapy-related side effects could occur. In this study, we explored the effects of AFPE on papillary (TPC-1) and anaplastic (CAL62) thyroid cancer cell line proliferation and viability. We found that AFPE exposure induced a reduction of cell proliferation and cell viability in dose-dependent manner. The effect was associated with the reduction of phosphorylation of Rb protein. To study the mechanisms underlying the biological effects of AFPE treatment in thyroid cancer cells, we investigated the modulation of miRNA (miR) expression. We found that AFPE treatment increased the expression of the miR-141, miR-145, miR-200a-5p, miR-425, and miR-551b-5p. Additionally, since natural polyphenols could exert their beneficial effects through the antioxidant properties, we investigated this aspect, and we found that AFPE treatment reduced the production of reactive oxygen species (ROS) in CAL62 cells. Moreover, AFPE pretreatment protects against hydrogen peroxide-induced oxidative stress in thyroid cancer cell lines. Taken together, our findings suggest that AFPE, by acting at micromolar concentration in thyroid cancer cell lines, may be considered a promising adjuvant natural agent for thyroid cancer treatment approach. Hindawi 2022-02-17 /pmc/articles/PMC8872649/ /pubmed/35222800 http://dx.doi.org/10.1155/2022/6268755 Text en Copyright © 2022 Francesca Maria Orlandella et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Orlandella, Francesca Maria Mirabelli, Peppino De Stefano, Anna Elisa Iervolino, Paola Lucia Chiara Luciano, Neila D'Angelo, Stefania Salvatore, Giuliana Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines |
title | Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines |
title_full | Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines |
title_fullStr | Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines |
title_full_unstemmed | Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines |
title_short | Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines |
title_sort | effects of annurca flesh apple polyphenols in human thyroid cancer cell lines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872649/ https://www.ncbi.nlm.nih.gov/pubmed/35222800 http://dx.doi.org/10.1155/2022/6268755 |
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