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Sharp, localized phase transitions in single neuronal cells
The origin of nonlinear responses in cells has been suggested to be crucial for various cell functions including the propagation of the nervous impulse. In physics, nonlinear behavior often originates from phase transitions. Evidence for such transitions on the single-cell level, however, has so far...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872731/ https://www.ncbi.nlm.nih.gov/pubmed/35165183 http://dx.doi.org/10.1073/pnas.2117521119 |
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author | Fedosejevs, Carina S. Schneider, Matthias F. |
author_facet | Fedosejevs, Carina S. Schneider, Matthias F. |
author_sort | Fedosejevs, Carina S. |
collection | PubMed |
description | The origin of nonlinear responses in cells has been suggested to be crucial for various cell functions including the propagation of the nervous impulse. In physics, nonlinear behavior often originates from phase transitions. Evidence for such transitions on the single-cell level, however, has so far not been provided, leaving the field unattended by the biological community. Here, we demonstrate that single cells of a human neuronal cell line display all optical features of a sharp, highly nonlinear phase transition within their membrane. The transition is reversible and does not originate from protein denaturation. Triggered by temperature and modified by pH here, a thermodynamic approach strongly suggests that similar nonlinear state changes can be induced by other variables such as calcium or mechanical stress. At least in lipid membranes, such state changes are accompanied by significant changes in permeability, enzyme activity, elastic, and electrical properties. |
format | Online Article Text |
id | pubmed-8872731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-88727312022-08-14 Sharp, localized phase transitions in single neuronal cells Fedosejevs, Carina S. Schneider, Matthias F. Proc Natl Acad Sci U S A Physical Sciences The origin of nonlinear responses in cells has been suggested to be crucial for various cell functions including the propagation of the nervous impulse. In physics, nonlinear behavior often originates from phase transitions. Evidence for such transitions on the single-cell level, however, has so far not been provided, leaving the field unattended by the biological community. Here, we demonstrate that single cells of a human neuronal cell line display all optical features of a sharp, highly nonlinear phase transition within their membrane. The transition is reversible and does not originate from protein denaturation. Triggered by temperature and modified by pH here, a thermodynamic approach strongly suggests that similar nonlinear state changes can be induced by other variables such as calcium or mechanical stress. At least in lipid membranes, such state changes are accompanied by significant changes in permeability, enzyme activity, elastic, and electrical properties. National Academy of Sciences 2022-02-14 2022-02-22 /pmc/articles/PMC8872731/ /pubmed/35165183 http://dx.doi.org/10.1073/pnas.2117521119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Physical Sciences Fedosejevs, Carina S. Schneider, Matthias F. Sharp, localized phase transitions in single neuronal cells |
title | Sharp, localized phase transitions in single neuronal cells |
title_full | Sharp, localized phase transitions in single neuronal cells |
title_fullStr | Sharp, localized phase transitions in single neuronal cells |
title_full_unstemmed | Sharp, localized phase transitions in single neuronal cells |
title_short | Sharp, localized phase transitions in single neuronal cells |
title_sort | sharp, localized phase transitions in single neuronal cells |
topic | Physical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872731/ https://www.ncbi.nlm.nih.gov/pubmed/35165183 http://dx.doi.org/10.1073/pnas.2117521119 |
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