Protocols to monitor TFEB activation following lysosomal damage in cultured cells using microscopy and immunoblotting

Following lysosomal damage, activation and nuclear translocation of transcription factor EB (TFEB) is the key event to maintain lysosomal homeostasis. Here, we describe steps to induce lysosomal damage in HeLa cells. This can be followed by monitoring the changes in TFEB localization using widefield...

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Detalles Bibliográficos
Autores principales: Ogura, Monami, Shima, Takayuki, Yoshimori, Tamotsu, Nakamura, Shuhei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8872846/
https://www.ncbi.nlm.nih.gov/pubmed/35243365
http://dx.doi.org/10.1016/j.xpro.2021.101018

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