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Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice
Colorectal cancer cells can transfer the oncogene KRAS to distant cells, predisposing them to malignant transformation (Genometastasis Theory). This process could contribute to liver metastasis; besides, hepatic progenitor cells (HPCs) have been found to be involved in liver malignant neoplasms. The...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8873244/ https://www.ncbi.nlm.nih.gov/pubmed/35210455 http://dx.doi.org/10.1038/s41598-022-06427-w |
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author | Olivera-Salazar, Rocío García-Arranz, Mariano Sánchez, Aránzazu Olmedillas-López, Susana Vega-Clemente, Luz Serrano, Luis Javier Herrera, Blanca García-Olmo, Damián |
author_facet | Olivera-Salazar, Rocío García-Arranz, Mariano Sánchez, Aránzazu Olmedillas-López, Susana Vega-Clemente, Luz Serrano, Luis Javier Herrera, Blanca García-Olmo, Damián |
author_sort | Olivera-Salazar, Rocío |
collection | PubMed |
description | Colorectal cancer cells can transfer the oncogene KRAS to distant cells, predisposing them to malignant transformation (Genometastasis Theory). This process could contribute to liver metastasis; besides, hepatic progenitor cells (HPCs) have been found to be involved in liver malignant neoplasms. The objective of this study is to determine if mouse HPCs—Oval cells (OCs)—are susceptible to incorporate Kras GAT (G12D) mutation from mouse colorectal cancer cell line CT26.WT and if OCs with the incorporated mutation behave like malignant cells. To achieve this, three lines of OCs in different conditions were exposed to CT26.WT cells through transwell co-culture for a week. The presence of Kras(G12D) and capacity to form tumors were analyzed in treated samples by droplet digital PCR and colony-forming assays, respectively. The results showed that the Kras(G12D) mutation was detected in hepatic culture conditions of undifferentiated OCs and these cells were capable of forming tumors in vitro. Therefore, OCs are susceptible to malignant transformation by horizontal transfer of DNA with Kras(G12D) mutation in an undifferentiated condition associated with the liver microenvironment. This study contributes to a new step in the understanding of the colorectal metastatic process. |
format | Online Article Text |
id | pubmed-8873244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-88732442022-02-25 Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice Olivera-Salazar, Rocío García-Arranz, Mariano Sánchez, Aránzazu Olmedillas-López, Susana Vega-Clemente, Luz Serrano, Luis Javier Herrera, Blanca García-Olmo, Damián Sci Rep Article Colorectal cancer cells can transfer the oncogene KRAS to distant cells, predisposing them to malignant transformation (Genometastasis Theory). This process could contribute to liver metastasis; besides, hepatic progenitor cells (HPCs) have been found to be involved in liver malignant neoplasms. The objective of this study is to determine if mouse HPCs—Oval cells (OCs)—are susceptible to incorporate Kras GAT (G12D) mutation from mouse colorectal cancer cell line CT26.WT and if OCs with the incorporated mutation behave like malignant cells. To achieve this, three lines of OCs in different conditions were exposed to CT26.WT cells through transwell co-culture for a week. The presence of Kras(G12D) and capacity to form tumors were analyzed in treated samples by droplet digital PCR and colony-forming assays, respectively. The results showed that the Kras(G12D) mutation was detected in hepatic culture conditions of undifferentiated OCs and these cells were capable of forming tumors in vitro. Therefore, OCs are susceptible to malignant transformation by horizontal transfer of DNA with Kras(G12D) mutation in an undifferentiated condition associated with the liver microenvironment. This study contributes to a new step in the understanding of the colorectal metastatic process. Nature Publishing Group UK 2022-02-24 /pmc/articles/PMC8873244/ /pubmed/35210455 http://dx.doi.org/10.1038/s41598-022-06427-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Olivera-Salazar, Rocío García-Arranz, Mariano Sánchez, Aránzazu Olmedillas-López, Susana Vega-Clemente, Luz Serrano, Luis Javier Herrera, Blanca García-Olmo, Damián Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice |
title | Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice |
title_full | Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice |
title_fullStr | Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice |
title_full_unstemmed | Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice |
title_short | Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice |
title_sort | oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8873244/ https://www.ncbi.nlm.nih.gov/pubmed/35210455 http://dx.doi.org/10.1038/s41598-022-06427-w |
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