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Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D

Neisseria gonorrhoeae is a human-specific pathogen that causes gonorrhea, the second most common sexually transmitted infection worldwide. Disease progression, drug discovery, and basic host-pathogen interactions are studied using different approaches, which rely on models ranging from 2D cell cultu...

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Autores principales: Heydarian, Motaharehsadat, Rühl, Eva, Rawal, Ravisha, Kozjak-Pavlovic, Vera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8873371/
https://www.ncbi.nlm.nih.gov/pubmed/35223556
http://dx.doi.org/10.3389/fcimb.2022.840122
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author Heydarian, Motaharehsadat
Rühl, Eva
Rawal, Ravisha
Kozjak-Pavlovic, Vera
author_facet Heydarian, Motaharehsadat
Rühl, Eva
Rawal, Ravisha
Kozjak-Pavlovic, Vera
author_sort Heydarian, Motaharehsadat
collection PubMed
description Neisseria gonorrhoeae is a human-specific pathogen that causes gonorrhea, the second most common sexually transmitted infection worldwide. Disease progression, drug discovery, and basic host-pathogen interactions are studied using different approaches, which rely on models ranging from 2D cell culture to complex 3D tissues and animals. In this review, we discuss the models used in N. gonorrhoeae research. We address both in vivo (animal) and in vitro cell culture models, discussing the pros and cons of each and outlining the recent advancements in the field of three-dimensional tissue models. From simple 2D monoculture to complex advanced 3D tissue models, we provide an overview of the relevant methodology and its application. Finally, we discuss future directions in the exciting field of 3D tissue models and how they can be applied for studying the interaction of N. gonorrhoeae with host cells under conditions closely resembling those found at the native sites of infection.
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spelling pubmed-88733712022-02-26 Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D Heydarian, Motaharehsadat Rühl, Eva Rawal, Ravisha Kozjak-Pavlovic, Vera Front Cell Infect Microbiol Cellular and Infection Microbiology Neisseria gonorrhoeae is a human-specific pathogen that causes gonorrhea, the second most common sexually transmitted infection worldwide. Disease progression, drug discovery, and basic host-pathogen interactions are studied using different approaches, which rely on models ranging from 2D cell culture to complex 3D tissues and animals. In this review, we discuss the models used in N. gonorrhoeae research. We address both in vivo (animal) and in vitro cell culture models, discussing the pros and cons of each and outlining the recent advancements in the field of three-dimensional tissue models. From simple 2D monoculture to complex advanced 3D tissue models, we provide an overview of the relevant methodology and its application. Finally, we discuss future directions in the exciting field of 3D tissue models and how they can be applied for studying the interaction of N. gonorrhoeae with host cells under conditions closely resembling those found at the native sites of infection. Frontiers Media S.A. 2022-02-11 /pmc/articles/PMC8873371/ /pubmed/35223556 http://dx.doi.org/10.3389/fcimb.2022.840122 Text en Copyright © 2022 Heydarian, Rühl, Rawal and Kozjak-Pavlovic https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Heydarian, Motaharehsadat
Rühl, Eva
Rawal, Ravisha
Kozjak-Pavlovic, Vera
Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D
title Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D
title_full Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D
title_fullStr Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D
title_full_unstemmed Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D
title_short Tissue Models for Neisseria gonorrhoeae Research—From 2D to 3D
title_sort tissue models for neisseria gonorrhoeae research—from 2d to 3d
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8873371/
https://www.ncbi.nlm.nih.gov/pubmed/35223556
http://dx.doi.org/10.3389/fcimb.2022.840122
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