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Real-time tracking of bioluminescent influenza A virus infection in mice
Despite the availability of vaccines and antiviral therapies, seasonal influenza infections cause 400,000 human deaths on average per year. Low vaccine coverage and the occurrence of drug-resistant viral strains highlight the need for new and improved countermeasures. While influenza A virus (IAV) e...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8873407/ https://www.ncbi.nlm.nih.gov/pubmed/35210462 http://dx.doi.org/10.1038/s41598-022-06667-w |
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author | Kim, Jin H. Bryant, Hannah Fiedler, Edward Cao, TuAnh Rayner, Jonathan O. |
author_facet | Kim, Jin H. Bryant, Hannah Fiedler, Edward Cao, TuAnh Rayner, Jonathan O. |
author_sort | Kim, Jin H. |
collection | PubMed |
description | Despite the availability of vaccines and antiviral therapies, seasonal influenza infections cause 400,000 human deaths on average per year. Low vaccine coverage and the occurrence of drug-resistant viral strains highlight the need for new and improved countermeasures. While influenza A virus (IAV) engineered to express a reporter gene may serve as a valuable tool for real-time tracking of viral infection, reporter gene insertion into IAV typically attenuates viral pathogenicity, hindering its application to research. Here, we demonstrate that lethal or even sublethal doses of bioluminescent IAV carrying the NanoLuc gene in the C-terminus of PB2 can be tracked in real-time in live mice without compromising pathogenicity. Real-time tracking of this bioluminescent IAV enables spatiotemporal viral replication tracking in animals that will facilitate the development of countermeasures by enhancing the interpretation of clinical signs and prognosis while also allowing less animal usage. |
format | Online Article Text |
id | pubmed-8873407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-88734072022-02-25 Real-time tracking of bioluminescent influenza A virus infection in mice Kim, Jin H. Bryant, Hannah Fiedler, Edward Cao, TuAnh Rayner, Jonathan O. Sci Rep Article Despite the availability of vaccines and antiviral therapies, seasonal influenza infections cause 400,000 human deaths on average per year. Low vaccine coverage and the occurrence of drug-resistant viral strains highlight the need for new and improved countermeasures. While influenza A virus (IAV) engineered to express a reporter gene may serve as a valuable tool for real-time tracking of viral infection, reporter gene insertion into IAV typically attenuates viral pathogenicity, hindering its application to research. Here, we demonstrate that lethal or even sublethal doses of bioluminescent IAV carrying the NanoLuc gene in the C-terminus of PB2 can be tracked in real-time in live mice without compromising pathogenicity. Real-time tracking of this bioluminescent IAV enables spatiotemporal viral replication tracking in animals that will facilitate the development of countermeasures by enhancing the interpretation of clinical signs and prognosis while also allowing less animal usage. Nature Publishing Group UK 2022-02-24 /pmc/articles/PMC8873407/ /pubmed/35210462 http://dx.doi.org/10.1038/s41598-022-06667-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kim, Jin H. Bryant, Hannah Fiedler, Edward Cao, TuAnh Rayner, Jonathan O. Real-time tracking of bioluminescent influenza A virus infection in mice |
title | Real-time tracking of bioluminescent influenza A virus infection in mice |
title_full | Real-time tracking of bioluminescent influenza A virus infection in mice |
title_fullStr | Real-time tracking of bioluminescent influenza A virus infection in mice |
title_full_unstemmed | Real-time tracking of bioluminescent influenza A virus infection in mice |
title_short | Real-time tracking of bioluminescent influenza A virus infection in mice |
title_sort | real-time tracking of bioluminescent influenza a virus infection in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8873407/ https://www.ncbi.nlm.nih.gov/pubmed/35210462 http://dx.doi.org/10.1038/s41598-022-06667-w |
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