Cell-Penetrating Botulinum Neurotoxin Type A With Improved Cellular Uptake and Therapeutic Index

Botulinum neurotoxin serotype A (BoNTA) is widely used for treating neuromuscular disorders. Despite of the various marketed products, BoNTA is known to have small therapeutic index ranging from 5 to 15. In the present study, we designed and characterized engineered BoNTA proteins with fusion of cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Wei, Xuan, Li, Lu, Wu, Yiwen, Liu, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874009/
https://www.ncbi.nlm.nih.gov/pubmed/35223792
http://dx.doi.org/10.3389/fbioe.2022.828427
_version_ 1784657588081655808
author Wei, Xuan
Li, Lu
Wu, Yiwen
Liu, Jia
author_facet Wei, Xuan
Li, Lu
Wu, Yiwen
Liu, Jia
author_sort Wei, Xuan
collection PubMed
description Botulinum neurotoxin serotype A (BoNTA) is widely used for treating neuromuscular disorders. Despite of the various marketed products, BoNTA is known to have small therapeutic index ranging from 5 to 15. In the present study, we designed and characterized engineered BoNTA proteins with fusion of cell-penetrating peptides (CPPs). We have shown that CPPs, particularly a recently reported zinc finger protein could improve the cellular uptake and intramuscular therapeutic index of BoNTA. Our study has shed the light on developing next-generation neuromuscular modulators using CPP fusion.
format Online
Article
Text
id pubmed-8874009
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88740092022-02-26 Cell-Penetrating Botulinum Neurotoxin Type A With Improved Cellular Uptake and Therapeutic Index Wei, Xuan Li, Lu Wu, Yiwen Liu, Jia Front Bioeng Biotechnol Bioengineering and Biotechnology Botulinum neurotoxin serotype A (BoNTA) is widely used for treating neuromuscular disorders. Despite of the various marketed products, BoNTA is known to have small therapeutic index ranging from 5 to 15. In the present study, we designed and characterized engineered BoNTA proteins with fusion of cell-penetrating peptides (CPPs). We have shown that CPPs, particularly a recently reported zinc finger protein could improve the cellular uptake and intramuscular therapeutic index of BoNTA. Our study has shed the light on developing next-generation neuromuscular modulators using CPP fusion. Frontiers Media S.A. 2022-02-11 /pmc/articles/PMC8874009/ /pubmed/35223792 http://dx.doi.org/10.3389/fbioe.2022.828427 Text en Copyright © 2022 Wei, Li, Wu and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wei, Xuan
Li, Lu
Wu, Yiwen
Liu, Jia
Cell-Penetrating Botulinum Neurotoxin Type A With Improved Cellular Uptake and Therapeutic Index
title Cell-Penetrating Botulinum Neurotoxin Type A With Improved Cellular Uptake and Therapeutic Index
title_full Cell-Penetrating Botulinum Neurotoxin Type A With Improved Cellular Uptake and Therapeutic Index
title_fullStr Cell-Penetrating Botulinum Neurotoxin Type A With Improved Cellular Uptake and Therapeutic Index
title_full_unstemmed Cell-Penetrating Botulinum Neurotoxin Type A With Improved Cellular Uptake and Therapeutic Index
title_short Cell-Penetrating Botulinum Neurotoxin Type A With Improved Cellular Uptake and Therapeutic Index
title_sort cell-penetrating botulinum neurotoxin type a with improved cellular uptake and therapeutic index
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874009/
https://www.ncbi.nlm.nih.gov/pubmed/35223792
http://dx.doi.org/10.3389/fbioe.2022.828427
work_keys_str_mv AT weixuan cellpenetratingbotulinumneurotoxintypeawithimprovedcellularuptakeandtherapeuticindex
AT lilu cellpenetratingbotulinumneurotoxintypeawithimprovedcellularuptakeandtherapeuticindex
AT wuyiwen cellpenetratingbotulinumneurotoxintypeawithimprovedcellularuptakeandtherapeuticindex
AT liujia cellpenetratingbotulinumneurotoxintypeawithimprovedcellularuptakeandtherapeuticindex

Ejemplares similares