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The influence of a biofilm‐dispersing wound gel on the wound healing process

Topical antimicrobials that reduce the bacterial bioburden within a chronically‐infected wound may have helpful or harmful effects on the healing process. We used murine models of full‐thickness skin wounds to determine the effects of the novel biofilm‐dispersing wound gel (BDWG) and its gel base on...

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Autores principales: Bounds, Kayla, Colmer‐Hamood, Jane A., Myntti, Matthew, Jeter, Randall M., Hamood, Abdul N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874046/
https://www.ncbi.nlm.nih.gov/pubmed/34263993
http://dx.doi.org/10.1111/iwj.13653
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author Bounds, Kayla
Colmer‐Hamood, Jane A.
Myntti, Matthew
Jeter, Randall M.
Hamood, Abdul N.
author_facet Bounds, Kayla
Colmer‐Hamood, Jane A.
Myntti, Matthew
Jeter, Randall M.
Hamood, Abdul N.
author_sort Bounds, Kayla
collection PubMed
description Topical antimicrobials that reduce the bacterial bioburden within a chronically‐infected wound may have helpful or harmful effects on the healing process. We used murine models of full‐thickness skin wounds to determine the effects of the novel biofilm‐dispersing wound gel (BDWG) and its gel base on the healing of uninfected wounds. The rate of wound closure over 19 days was comparable among the BDWG‐treated (BT) wounds and the controls. Compared with the controls, histology of the BT wounds showed formation of a stable blood clot at day 1, more neovascularisation and reepithelialisation at day 3, and more organised healing at day 7. Fluorescence‐activated cell sorting analysis showed a lower percentage of neutrophils in wounded tissues of the BT group at days 1 and 3, and significantly more M2 macrophages at day 3. Levels of proinflammatory cytokines and chemokines were increased over the uninjured baseline within the wounds of all treatment groups but the levels were significantly lower in the BT group at day 1, modulating the inflammatory response. Our results suggest that BDWG does not interfere with the wound healing process and may enhance it by lowering inflammation and allowing transition to the proliferative stage of wound healing by day 3.
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spelling pubmed-88740462022-02-28 The influence of a biofilm‐dispersing wound gel on the wound healing process Bounds, Kayla Colmer‐Hamood, Jane A. Myntti, Matthew Jeter, Randall M. Hamood, Abdul N. Int Wound J Original Articles Topical antimicrobials that reduce the bacterial bioburden within a chronically‐infected wound may have helpful or harmful effects on the healing process. We used murine models of full‐thickness skin wounds to determine the effects of the novel biofilm‐dispersing wound gel (BDWG) and its gel base on the healing of uninfected wounds. The rate of wound closure over 19 days was comparable among the BDWG‐treated (BT) wounds and the controls. Compared with the controls, histology of the BT wounds showed formation of a stable blood clot at day 1, more neovascularisation and reepithelialisation at day 3, and more organised healing at day 7. Fluorescence‐activated cell sorting analysis showed a lower percentage of neutrophils in wounded tissues of the BT group at days 1 and 3, and significantly more M2 macrophages at day 3. Levels of proinflammatory cytokines and chemokines were increased over the uninjured baseline within the wounds of all treatment groups but the levels were significantly lower in the BT group at day 1, modulating the inflammatory response. Our results suggest that BDWG does not interfere with the wound healing process and may enhance it by lowering inflammation and allowing transition to the proliferative stage of wound healing by day 3. Blackwell Publishing Ltd 2021-07-15 /pmc/articles/PMC8874046/ /pubmed/34263993 http://dx.doi.org/10.1111/iwj.13653 Text en © 2021 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Bounds, Kayla
Colmer‐Hamood, Jane A.
Myntti, Matthew
Jeter, Randall M.
Hamood, Abdul N.
The influence of a biofilm‐dispersing wound gel on the wound healing process
title The influence of a biofilm‐dispersing wound gel on the wound healing process
title_full The influence of a biofilm‐dispersing wound gel on the wound healing process
title_fullStr The influence of a biofilm‐dispersing wound gel on the wound healing process
title_full_unstemmed The influence of a biofilm‐dispersing wound gel on the wound healing process
title_short The influence of a biofilm‐dispersing wound gel on the wound healing process
title_sort influence of a biofilm‐dispersing wound gel on the wound healing process
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874046/
https://www.ncbi.nlm.nih.gov/pubmed/34263993
http://dx.doi.org/10.1111/iwj.13653
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