Cargando…
Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity
SARS-CoV-2 spike mRNA vaccines have shown remarkable clinical efficacy in the general population, although the nature of T-cell priming is not fully understood. We performed longitudinal spike-, membrane-, and nucleocapsid-specific T-cell analysis in individuals with past infection and infection-naï...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874124/ https://www.ncbi.nlm.nih.gov/pubmed/35222312 http://dx.doi.org/10.3389/fmicb.2021.812729 |
_version_ | 1784657613745553408 |
---|---|
author | Vallejo, Alejandro Martín-Hondarza, Adrián Gómez, Sandra Velasco, Héctor Vizcarra, Pilar Haemmerle, Johannes Casado, José L. |
author_facet | Vallejo, Alejandro Martín-Hondarza, Adrián Gómez, Sandra Velasco, Héctor Vizcarra, Pilar Haemmerle, Johannes Casado, José L. |
author_sort | Vallejo, Alejandro |
collection | PubMed |
description | SARS-CoV-2 spike mRNA vaccines have shown remarkable clinical efficacy in the general population, although the nature of T-cell priming is not fully understood. We performed longitudinal spike-, membrane-, and nucleocapsid-specific T-cell analysis in individuals with past infection and infection-naïve individuals with cross-reactivity. We found an additional enhancement of T-cell response to the structural membrane (M) and nucleocapsid (N) SARS-CoV-2 proteins after mRNA vaccine in these individuals. Thus, despite the spike-specific response, we found that the first dose of the vaccine boosted a significant CD8 cell response to M and N proteins, whereas no cellular response to those proteins was found in infection-naïve individuals without pre-existing cross-reactivity who were tested for eventual asymptomatic infection. These findings highlight the additional benefit of mRNA vaccines as broad boosters of cellular responses to different viral epitopes in these individuals and suggest extended protection to other viral variants. |
format | Online Article Text |
id | pubmed-8874124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88741242022-02-26 Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity Vallejo, Alejandro Martín-Hondarza, Adrián Gómez, Sandra Velasco, Héctor Vizcarra, Pilar Haemmerle, Johannes Casado, José L. Front Microbiol Microbiology SARS-CoV-2 spike mRNA vaccines have shown remarkable clinical efficacy in the general population, although the nature of T-cell priming is not fully understood. We performed longitudinal spike-, membrane-, and nucleocapsid-specific T-cell analysis in individuals with past infection and infection-naïve individuals with cross-reactivity. We found an additional enhancement of T-cell response to the structural membrane (M) and nucleocapsid (N) SARS-CoV-2 proteins after mRNA vaccine in these individuals. Thus, despite the spike-specific response, we found that the first dose of the vaccine boosted a significant CD8 cell response to M and N proteins, whereas no cellular response to those proteins was found in infection-naïve individuals without pre-existing cross-reactivity who were tested for eventual asymptomatic infection. These findings highlight the additional benefit of mRNA vaccines as broad boosters of cellular responses to different viral epitopes in these individuals and suggest extended protection to other viral variants. Frontiers Media S.A. 2022-02-11 /pmc/articles/PMC8874124/ /pubmed/35222312 http://dx.doi.org/10.3389/fmicb.2021.812729 Text en Copyright © 2022 Vallejo, Martín-Hondarza, Gómez, Velasco, Vizcarra, Haemmerle and Casado. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Vallejo, Alejandro Martín-Hondarza, Adrián Gómez, Sandra Velasco, Héctor Vizcarra, Pilar Haemmerle, Johannes Casado, José L. Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity |
title | Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity |
title_full | Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity |
title_fullStr | Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity |
title_full_unstemmed | Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity |
title_short | Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity |
title_sort | cellular responses to membrane and nucleocapsid viral proteins are also boosted after sars-cov-2 spike mrna vaccination in individuals with either past infection or cross-reactivity |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874124/ https://www.ncbi.nlm.nih.gov/pubmed/35222312 http://dx.doi.org/10.3389/fmicb.2021.812729 |
work_keys_str_mv | AT vallejoalejandro cellularresponsestomembraneandnucleocapsidviralproteinsarealsoboostedaftersarscov2spikemrnavaccinationinindividualswitheitherpastinfectionorcrossreactivity AT martinhondarzaadrian cellularresponsestomembraneandnucleocapsidviralproteinsarealsoboostedaftersarscov2spikemrnavaccinationinindividualswitheitherpastinfectionorcrossreactivity AT gomezsandra cellularresponsestomembraneandnucleocapsidviralproteinsarealsoboostedaftersarscov2spikemrnavaccinationinindividualswitheitherpastinfectionorcrossreactivity AT velascohector cellularresponsestomembraneandnucleocapsidviralproteinsarealsoboostedaftersarscov2spikemrnavaccinationinindividualswitheitherpastinfectionorcrossreactivity AT vizcarrapilar cellularresponsestomembraneandnucleocapsidviralproteinsarealsoboostedaftersarscov2spikemrnavaccinationinindividualswitheitherpastinfectionorcrossreactivity AT haemmerlejohannes cellularresponsestomembraneandnucleocapsidviralproteinsarealsoboostedaftersarscov2spikemrnavaccinationinindividualswitheitherpastinfectionorcrossreactivity AT casadojosel cellularresponsestomembraneandnucleocapsidviralproteinsarealsoboostedaftersarscov2spikemrnavaccinationinindividualswitheitherpastinfectionorcrossreactivity |