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Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity

SARS-CoV-2 spike mRNA vaccines have shown remarkable clinical efficacy in the general population, although the nature of T-cell priming is not fully understood. We performed longitudinal spike-, membrane-, and nucleocapsid-specific T-cell analysis in individuals with past infection and infection-naï...

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Autores principales: Vallejo, Alejandro, Martín-Hondarza, Adrián, Gómez, Sandra, Velasco, Héctor, Vizcarra, Pilar, Haemmerle, Johannes, Casado, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874124/
https://www.ncbi.nlm.nih.gov/pubmed/35222312
http://dx.doi.org/10.3389/fmicb.2021.812729
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author Vallejo, Alejandro
Martín-Hondarza, Adrián
Gómez, Sandra
Velasco, Héctor
Vizcarra, Pilar
Haemmerle, Johannes
Casado, José L.
author_facet Vallejo, Alejandro
Martín-Hondarza, Adrián
Gómez, Sandra
Velasco, Héctor
Vizcarra, Pilar
Haemmerle, Johannes
Casado, José L.
author_sort Vallejo, Alejandro
collection PubMed
description SARS-CoV-2 spike mRNA vaccines have shown remarkable clinical efficacy in the general population, although the nature of T-cell priming is not fully understood. We performed longitudinal spike-, membrane-, and nucleocapsid-specific T-cell analysis in individuals with past infection and infection-naïve individuals with cross-reactivity. We found an additional enhancement of T-cell response to the structural membrane (M) and nucleocapsid (N) SARS-CoV-2 proteins after mRNA vaccine in these individuals. Thus, despite the spike-specific response, we found that the first dose of the vaccine boosted a significant CD8 cell response to M and N proteins, whereas no cellular response to those proteins was found in infection-naïve individuals without pre-existing cross-reactivity who were tested for eventual asymptomatic infection. These findings highlight the additional benefit of mRNA vaccines as broad boosters of cellular responses to different viral epitopes in these individuals and suggest extended protection to other viral variants.
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spelling pubmed-88741242022-02-26 Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity Vallejo, Alejandro Martín-Hondarza, Adrián Gómez, Sandra Velasco, Héctor Vizcarra, Pilar Haemmerle, Johannes Casado, José L. Front Microbiol Microbiology SARS-CoV-2 spike mRNA vaccines have shown remarkable clinical efficacy in the general population, although the nature of T-cell priming is not fully understood. We performed longitudinal spike-, membrane-, and nucleocapsid-specific T-cell analysis in individuals with past infection and infection-naïve individuals with cross-reactivity. We found an additional enhancement of T-cell response to the structural membrane (M) and nucleocapsid (N) SARS-CoV-2 proteins after mRNA vaccine in these individuals. Thus, despite the spike-specific response, we found that the first dose of the vaccine boosted a significant CD8 cell response to M and N proteins, whereas no cellular response to those proteins was found in infection-naïve individuals without pre-existing cross-reactivity who were tested for eventual asymptomatic infection. These findings highlight the additional benefit of mRNA vaccines as broad boosters of cellular responses to different viral epitopes in these individuals and suggest extended protection to other viral variants. Frontiers Media S.A. 2022-02-11 /pmc/articles/PMC8874124/ /pubmed/35222312 http://dx.doi.org/10.3389/fmicb.2021.812729 Text en Copyright © 2022 Vallejo, Martín-Hondarza, Gómez, Velasco, Vizcarra, Haemmerle and Casado. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Vallejo, Alejandro
Martín-Hondarza, Adrián
Gómez, Sandra
Velasco, Héctor
Vizcarra, Pilar
Haemmerle, Johannes
Casado, José L.
Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity
title Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity
title_full Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity
title_fullStr Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity
title_full_unstemmed Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity
title_short Cellular Responses to Membrane and Nucleocapsid Viral Proteins Are Also Boosted After SARS-CoV-2 Spike mRNA Vaccination in Individuals With Either Past Infection or Cross-Reactivity
title_sort cellular responses to membrane and nucleocapsid viral proteins are also boosted after sars-cov-2 spike mrna vaccination in individuals with either past infection or cross-reactivity
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8874124/
https://www.ncbi.nlm.nih.gov/pubmed/35222312
http://dx.doi.org/10.3389/fmicb.2021.812729
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